DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ huang-2013-20 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264958X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions imex curation huang-2013-21 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264954X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions imex curation huang-2013-22 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264957X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions imex curation huang-2013-23 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264955X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions imex curation huang-2013-24 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264959X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions imex curation huang-2013-25 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264956X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions imex curation p05771-1 Protein kinase C beta type PKCB PRKCB1 PRKCB1 PRKCB protein protein human Homo sapiens Human MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV kpcd_human Protein kinase C delta type nPKC-delta PRKCD Tyrosine-protein kinase PRKCD PKCD protein protein human Homo sapiens Human MAPFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED ripk4_human Receptor-interacting serine/threonine-protein kinase 4 RIPK4 ANKRD3 DIK Ankyrin repeat domain-containing protein 3 PKC-delta-interacting protein kinase protein protein human Homo sapiens Human MEGDGGTPWALALLRTFDAGEFTGWEKVGSGGFGQVYKVRHVHWKTWLAIKCSPSLHVDDRERMELLEEAKKMEMAKFRYILPVYGICREPVGLVMEYMETGSLEKLLASEPLPWDLRFRIIHETAVGMNFLHCMAPPLLHLDLKPANILLDAHYHVKISDFGLAKCNGLSHSHDLSMDGLFGTIAYLPPERIREKSRLFDTKHDVYSFAIVIWGVLTQKKPFADEKNILHIMVKVVKGHRPELPPVCRARPRACSHLIRLMQRCWQGDPRVRPTFQGNGLNGELIRQVLAALLPVTGRWRSPGEGFRLESEVIIRVTCPLSSPQEITSETEDLCEKPDDEVKETAHDLDVKSPPEPRSEVVPARLKRASAPTFDNDYSLSELLSQLDSGVSQAVEGPEELSRSSSESKLPSSGSGKRLSGVSSVDSAFSSRGSLSLSFEREPSTSDLGTTDVQKKKLVDAIVSGDTSKLMKILQPQDVDLALDSGASLLHLAVEAGQEECAKWLLLNNANPNLSNRRGSTPLHMAVERRVRGVVELLLARKISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNASVNEVDFEGRTPMHVACQHGQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAMTSDGYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALHLAAAHGHSEVVEELVSADVIDLFDEQGLSALHLAAQGRHAQTVETLLRHGAHINLQSLKFQGGHGPAATLLRRSKT dvl2_human Segment polarity protein dishevelled homolog DVL-2 DSH homolog 2 DVL2 protein protein human Homo sapiens Human MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESEPSSRGGSLRRGGEASGTSDGGPPPSRGSTGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM lrp6_human Low-density lipoprotein receptor-related protein 6 LRP6 protein protein human Homo sapiens Human MGAVLRSLLACSFCVLLRAAPLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVFSHGLIYWSDVSEEAIKRTEFNKTESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIEVSNLDGSLRKVLFWQELDQPRAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFIIINSEIYWPNGLTLDYEEQKLYWADAKLNFIHKSNLDGTNRQAVVKGSLPHPFALTLFEDILYWTDWSTHSILACNKYTGEGLREIHSDIFSPMDIHAFSQQRQPNATNPCGIDNGGCSHLCLMSPVKPFYQCACPTGVKLLENGKTCKDGATELLLLARRTDLRRISLDTPDFTDIVLQLEDIRHAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSDRVVLVNTSLGWPNGLALDYDEGKIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTLLGDYVYWTDWQRRSIERVHKRSAEREVIIDQLPDLMGLKATNVHRVIGSNPCAEENGGCSHLCLYRPQGLRCACPIGFELISDMKTCIVPEAFLLFSRRADIRRISLETNNNNVAIPLTGVKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVVEFGLDYPEGMAVDWLGKNLYWADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGKPKIDRAAMDGSERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSNMLGLNREVIADDLPHPFGLTQYQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQSGWNECASSNGHCSHLCLAVPVGGFVCGCPAHYSLNADNRTCSAPTTFLLFSQKSAINRMVIDEQQSPDIILPIHSLRNVRAIDYDPLDKQLYWIDSRQNMIRKAQEDGSQGFTVVVSSVPSQNLEIQPYDLSIDIYSRYIYWTCEATNVINVTRLDGRSVGVVLKGEQDRPRAVVVNPEKGYMYFTNLQERSPKIERAALDGTEREVLFFSGLSKPIALALDSRLGKLFWADSDLRRIESSDLSGANRIVLEDSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSDIHAVKELNLQEYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGEPPTCSPQQFTCFTGEIDCIPVAWRCDGFTECEDHSDELNCPVCSESQFQCASGQCIDGALRCNGDANCQDKSDEKNCEVLCLIDQFRCANGQCIGKHKKCDHNVDCSDKSDELDCYPTEEPAPQATNTVGSVIGVIVTIFVSGTVYFICQRMLCPRMKGDGETMTNDYVVHGPASVPLGYVPHPSSLSGSLPGMSRGKSMISSLSIMGGSSGPPYDRAHVTGASSSSSSSTKGTYFPAILNPPPSPATERSHYTMEFGYSSNSPSTHRSYSYRPYSYRHFAPPTTPCSTDVCDSDYAPSRRMTSVATAKGYTSDLNYDSEPVPPPPTPRSQYLSAEENYESCPPSPYTERSYSHHLYPPPPSPCTDSS ripk4-prkcb-1 3250314 N/A 3250320 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3250321 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3250322 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human association association false false false dvl2-ripk4-1 3250315 N/A 3250322 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3250323 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false dvl2-ripk4-2 3250316 N/A 3250322 unspecified role unspecified role unspecified role unspecified role Region 2-300 Region 2-300 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3250323 unspecified role unspecified role unspecified role unspecified role Region 267-353 Region 267-353 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 436-505 Region 436-505 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O14641:p.Ser298Ala S298A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O14641:p.Ser480Ala S480A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false lrp6-ripk4-1 3250317 N/A 3250324 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3250322 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false dvl2-ripk4-3 3250318 N/A 3250322 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false in vitro In vitro N/A 3250323 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false in vitro In vitro physical association physical association false false false dvl2-ripk4-4 3250319 N/A 3250322 unspecified role unspecified role unspecified role unspecified role Region 2-300 Region 2-300 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P57078:p.Lys51Arg K51R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3250323 unspecified role unspecified role unspecified role unspecified role Region 267-353 Region 267-353 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 436-505 Region 436-505 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false