UniProt UniProt http://www.uniprot.org humphryes-2012-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Humphryes N., Leung WK., Argunhan B., Terentyev Y., Dvorackova M., Tsubouchi H. h.tsubouchi@sussex.ac.uk PLoS genetics 2012 imex curation Only protein-protein interactions imex curation humphryes-2012-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Humphryes N., Leung WK., Argunhan B., Terentyev Y., Dvorackova M., Tsubouchi H. h.tsubouchi@sussex.ac.uk PLoS genetics 2012 imex curation Only protein-protein interactions imex curation humphryes-2012-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Humphryes N., Leung WK., Argunhan B., Terentyev Y., Dvorackova M., Tsubouchi H. h.tsubouchi@sussex.ac.uk PLoS genetics 2012 imex curation Only protein-protein interactions imex curation ecm11_yeast Protein ECM11 ECM11 YDR446W D9461.31 Extracellular mutant protein 11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTVIKTEPTTEVTLYSPPSKESLSKDDAHRKKQNNKPPSSINSRSGPNKHKLAAKAPEKKINNTDKQDLSAFLLNPSLIVKPSESKKKENIVAYNDTPGIKTEHTAFQPLTPISKKRALKEKAASEKCDSFDLSRDEKPYIQKKSKTLSSVTEINSSEYKLSLNGENTSSPAKEKSQEPIENPGSYQKTRNYLFEKPDPLDTCLQDYSSMLPSNVAEEDQEYFISVADSTLEEWTNKGQEIIDQQFQLYQEIIKKRIELSYKFKGIISVINDRADALEEQGQQLEGKIKKVKTLANEILNII CB6890F4EE069232 gmc2_yeast Grand meiotic recombination cluster protein 2 GMC2 YLR445W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDTTEVPRQSSENDQDNNLERTNSLKSPDVTNNIPSLFKLAAEWQINNPQETFQNHILENDVLKKINEITHLIRESYKDLSSQDGMMSKQQQEKMDWDLFCTVPVNIIEQYTKDMDEIYEKMERLAKQQRLWCESAFQIDVERCGDSILNAETWMKKKERHLEYKNIEMERSANEIKETIQRLTDDR ecm11-gmc2-5 3321498 N/A 3321501 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3321502 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4E ecm11-gmc2-3 3321498 N/A 3321501 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3321502 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4E ecm11-gmc2-1 3321499 N/A 3321501 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell N/A 3321502 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association false false false Fig. 4D ecm11-gmc2-4 3321500 N/A 3321501 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3321502 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figs. 4A, 5B Colocalization observed in ZIP1-/- cells