DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lyons-2013-2 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Lyons NA., Fonslow BR., Diedrich JK., Yates JR., Morgan DO. DIP original experiment: DIP-251986X Nature structural & molecular biology 2013 IMEx Sequential primed kinases create a damage-responsive phosphodegron on Eco1. Lyons NA., Fonslow BR., Diedrich JK., Yates JR., Morgan DO. Only protein-protein interactions imex curation eco1_yeast N-acetyltransferase ECO1 ECO1 CTF7 YFR027W Establishment of cohesion protein 1 Chromosome transmission fidelity protein 7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKARKSQRKAGSKPNLIQSKLQVNNGSKSNKIVKCDKCEMSYSSTSIEDRAIHEKYHTLQLHGRKWSPNWGSIVYTERNHSRTVHLSRSTGTITPLNSSPLKKSSPSITHQEEKIVYVRPDKSNGEVRAMTEIMTLVNNELNAPHDENVIWNSTTEEKGKAFVYIRNDRAVGIIIIENLYGGNGKTSSRGRWMVYDSRRLVQNVYPDFKIGISRIWVCRTARKLGIATKLIDVARENIVYGEVIPRYQVAWSQPTDSGGKLASKYNGIMHKSGKLLLPVYI 0AF1B59BE9E71818 mck1_yeast Protein kinase MCK1 YPK1 YNL307C N0392 MCK1 Meiosis and centromere regulatory kinase protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTEEQNGVPLQRGSEFIADDVTSNKSNNTRRMLVKEYRKIGRGAFGTVVQAYLTQDKKNWLGPFAIKKVPAHTEYKSRELQILRIADHPNIVKLQYFFTHLSPQDNKVYQHLAMECLPETLQIEINRYVTNKLEMPLKHIRLYTYQIARGMLYLHGLGVCHRDIKPSNVLVDPETGVLKICDFGSAKKLEHNQPSISYICSRFYRAPELIIGCTQYTTQIDIWGLGCVMGEMLIGKAIFQGQEPLLQLREIAKLLGPPDKRFIFFSNPAYDGPLFSKPLFSGSSQQRFEKYFGHSGPDGIDLLMKILVYEPQQRLSPRRILAHQFFNELRNDDTFLPRGFTEPIKLPNLFDFNDFELQILGEFADKIKPTKVAE BCF0C776B6E3841B eco1-mck1-1 3249540 N/A 3249541 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 91-103 Region 91-103 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pS98 pS98 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false P43605:p.Thr94Ala T94A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3249542 enzyme enzyme unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast phosphorylation phosphorylation reaction phosphorylation false false false