DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2013-15 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252341X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions imex curation wang-2013-16 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252345X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions imex curation wang-2013-17 in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252347X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions imex curation wang-2013-18 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252343X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions imex curation wang-2013-19 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252346X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions imex curation wang-2013-20 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252342X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions imex curation wang-2013-21 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252344X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions imex curation u2af2_human Splicing factor U2AF 65 kDa subunit U2AF2 U2AF65 U2 snRNP auxiliary factor large subunit U2 auxiliary factor 65 kDa subunit protein protein human Homo sapiens Human MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW sf01_human Splicing factor 1 Zinc finger protein 162 Transcription factor ZFM1 Zinc finger gene in MEN1 locus Mammalian branch point-binding protein SF1 ZFM1 ZNF162 protein protein human Homo sapiens Human MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTANTMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCTKCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPANNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGPPPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPPPPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQMQGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPPAPPPPPPQN sf1-u2af2-9 3249058 N/A 3249065 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3249066 unspecified role unspecified role bait bait Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q15637:p.Trp22Ala W22A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q15637:p.[Ser80Ala;Ser82Ala] S80A/S82A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q15637:p.[Arg93Glu;Arg97Glu;Arg100Glu] R93E/R97E/R100E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q15637:p.[Ile40Arg;Ile53Arg] I40R/I53R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q15637:p.Arg46Asp R46D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false sf1-u2af2-10 3249059 N/A 3249065 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3249066 unspecified role unspecified role unspecified role unspecified role Region 14-132 Region 14-132 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sf1-u2af2-11 3249060 N/A 3249065 unspecified role unspecified role unspecified role unspecified role Region 148-475 Region 148-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3249066 unspecified role unspecified role unspecified role unspecified role Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sf1-u2af2-12 3249061 N/A 3249065 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3249066 unspecified role unspecified role unspecified role unspecified role Region 14-132 Region 14-132 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sf1-u2af2-13 3249062 N/A 3249065 unspecified role unspecified role unspecified role unspecified role Region 148-475 Region 148-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3249066 unspecified role unspecified role unspecified role unspecified role Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sf1-u2af2-14 3249063 N/A 3249065 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3249066 unspecified role unspecified role unspecified role unspecified role Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sf1-u2af2-15 3249064 N/A 3249065 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3249066 unspecified role unspecified role unspecified role unspecified role Region 14-132 Region 14-132 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false