DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ straub-2012-1 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251214X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions imex curation straub-2012-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251219X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions imex curation straub-2012-3 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251217X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions imex curation straub-2012-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251215X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions imex curation straub-2012-5 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251218X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions imex curation straub-2012-6 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251216X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions imex curation nos3_mouse Nitric oxide synthase, endothelial Nos3 Ecnos Endothelial NOS EC-NOS NOS type III Constitutive NOS protein protein mouse Mus musculus Mouse MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSQAPAPPSPTRPAPDHSPPLTRPPDGPRFPRVKNWEVGSITYDTLSAQAQQDGPCTSRRCLGSLVFPRKLQSRPTQGPSPTEQLLGQARDFINQYYNSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRTAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLIRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEPPELFTLPPEMVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSSEIGMRDLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGAGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQAESLQLLPGLTHVHRRKMFQATILSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPSTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTYFLDITSPPSPRLLRLLSTLAEESSEQQELEALSQDPRRYEEWKWFSCPTLLEVLEQFPSVALPAPLILTQLPLLQPRYYSVSSAPSAHPGEIHLTIAVLAYRTQDGLGPLHYGVCSTWMSQLKAGDPVPCFIRGAPSFRLPPDPNLPCILVGPGTGIAPFRGFWQDRLHDIEIKGLQPAPMTLVFGCRCSQLDHLYRDEVLDAQQRGVFGQVLTAFSRDPGSPKTYVQDLLRTELAAEVHRVLCLEQGHMFVCGDVTMATSVLQTVQRILATEGGMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWSFDPPGPEIPGS hba_mouse Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin Hba Hba-a1 protein protein mouse Mus musculus Mouse MVLSGEDKSNIKAAWGKIGGHGAEYGAEALERMFASFPTTKTYFPHFDVSHGSAQVKGHGKKVADALASAAGHLDDLPGALSALSDLHAHKLRVDPVNFKLLSHCLLVTLASHHPADFTPAVHASLDKFLASVSTVLTSKYR nb5r3_human NADH-cytochrome b5 reductase 3 Diaphorase-1 CYB5R3 DIA1 protein protein human Homo sapiens Human MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF hba_human Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin HBA1 HBA2 protein protein human Homo sapiens Human MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR nb5r3_mouse NADH-cytochrome b5 reductase 3 Cyb5r3 Dia1 Diaphorase-1 protein protein mouse Mus musculus Mouse MGAQLSTLSHVVLSPVWFIYSLFMKLFQRSTPAITLENPDIKYPLRLIDKEVISPDTRRFRFALPSPQHILGLPIGQHIYLSTRIDGNLVIRPYTPVSSDDDKGFVDLVVKVYFKDTHPKFPAGGKMSQYLENMKIGDTIEFRGPNGLLVYQGKGKFAIRADKKSNPVVRTVKSVGMIAGGTGITPMLQVIRAVLKDPNDHTVCYLLFANQSEKDILLRPELEELRNEHSARFKLWYTVDKAPDAWDYSQGFVNEEMIRDHLPTPGEEPLILMCGPPPMIQFACLPNLERVGHPKERCFTF nos3_human Nitric oxide synthase, endothelial Endothelial NOS EC-NOS NOS type III Constitutive NOS NOS3 protein protein human Homo sapiens Human MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP hba-nos3-1 3246929 N/A 3246935 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3246936 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false hba1-cyb5r3-1 3246930 N/A 3246937 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3246938 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false hba-cyb5r3-1 3246931 N/A 3246939 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3246936 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false hba1-nos3-1 3246932 N/A 3246940 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3246938 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false cyb5r3-hba1-1 3246933 N/A 3246937 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3246938 unspecified role unspecified role prey prey human Homo sapiens Human direct interaction direct interaction false false false nos3-hba1-1 3246934 N/A 3246940 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3246938 unspecified role unspecified role prey prey human Homo sapiens Human direct interaction direct interaction false false false