DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ janssen-2012-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. DIP original experiment: DIP-251373X Nature structural & molecular biology 2012 IMEx Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex. Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. Only protein-protein interactions imex curation janssen-2012-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. DIP original experiment: DIP-251375X Nature structural & molecular biology 2012 IMEx Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex. Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. Only protein-protein interactions imex curation janssen-2012-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. DIP original experiment: DIP-251377X Nature structural & molecular biology 2012 IMEx Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex. Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. Only protein-protein interactions imex curation janssen-2012-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. DIP original experiment: DIP-251374X Nature structural & molecular biology 2012 IMEx Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex. Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. Only protein-protein interactions imex curation janssen-2012-5 human Homo sapiens Human x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. DIP original experiment: DIP-251376X Nature structural & molecular biology 2012 IMEx Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex. Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. Only protein-protein interactions imex curation janssen-2012-6 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. DIP original experiment: DIP-251378X Nature structural & molecular biology 2012 IMEx Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex. Janssen BJ., Malinauskas T., Weir GA., Cader MZ., Siebold C., Jones EY. Only protein-protein interactions imex curation sem3a_mouse Semaphorin-3A Sema3a Semad SemD Semaphorin III Semaphorin-D protein protein mouse Mus musculus Mouse MGWFTGIACLFWGVLLTARANYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLEAYNQTHLYACGTGAFHPICTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRPIMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEILLEEMTVFREPTTISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRNGDPLTHCSDLQHHDNHHGPSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRMGDHIIRTEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKIKEMSSSMTPSQKVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPRSV mint Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally nrp1_mouse Neuropilin-1 A5 protein Nrp1 Nrp protein protein mouse Mus musculus Mouse MERGLPLLCATLALALALAGAFRSDKCGGTIKIENPGYLTSPGYPHSYHPSEKCEWLIQAPEPYQRIMINFNPHFDLEDRDCKYDYVEVIDGENEGGRLWGKFCGKIAPSPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTAPTGVIKSPGFPEKYPNSLECTYIIFAPKMSEIILEFESFDLEQDSNPPGGMFCRYDRLEIWDGFPEVGPHIGRYCGQKTPGRIRSSSGVLSMVFYTDSAIAKEGFSANYSVLQSSISEDFKCMEALGMESGEIHSDQITASSQYGTNWSVERSRLNYPENGWTPGEDSYKEWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYRVDISSNGEDWISLKEGNKAIIFQGNTNPTDVVLGVFSKPLITRFVRIKPVSWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITASNQADRNWMPENIRLVTSRTGWALPPSPHPYTNEWLQVDLGDEKIVRGVIIQGGKHRENKVFMRKFKIAYSNNGSDWKTIMDDSKRKAKSFEGNNNYDTPELRTFSPLSTRFIRIYPERATHSGLGLRMELLGCEVEAPTAGPTTPNGNPVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTIIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDSHAQLRWSVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQSSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMVVGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPTDLDKKNTEIKIDETGSTPGYEGEGEGDKNISRKPGNVLKTLDPILITIIAMSALGVLLGAVCGVVLYCACWHNGMSERNLSALENYNFELVDGVKLKKDKLNPQSNYSEA plxa2_mouse Plexin-A2 Plxna2 Kiaa0463 protein protein mouse Mus musculus Mouse MEQRRFYLRAMQADNLSVVLLSVAWLLLARGTTGMPQYSTFHSENRDWTFNHLTVHRRTGAVYVGAINRVYKLTGNLTIQVAHKTGPEEDNKACYPPLIVQPCSEVLTLTNNVNKLLIIDYSENRLLACGSLYQGVCKLLRLDDLFILVEPSHKKEHYLSSVNKTGTMYGVIVRSEGEDGKLFIGTAVDGKQDYFPTLSSRKLPRDPESSAMLDYELHSDFVSSLIKIPSDTLALVSHFDIFYIYGFASGGFVYFLTVQPETPDGMAINSAGDLFYTSRIVRLCKDDPKFHSYVSLPFGCTRAGVEYRLLQAAYLAKPGEALAQAFNISSDEDVLFAIFSKGQKQYHHPPDDSALCAFPIRAINLQIKERLQSCYHGEGNLELNWLLGKDVQCTKAPVPIDDNFCGLDINQPLGGSTPVEGLTLYTTSRDRLTSVASYVYNGYSVVFVGTKSGKLKKIRADGPPHGGVQYEMVSVFKDGSPILRDMAFSINQLYLYVMSERQVTRVPVESCEQYTTCGECLSSGDPHCGWCALHNMCSRRDKCQRAWEANRFAASISQCMSLEVHPNSISVSDHSRLLSLVVNDAPNLSEGIACAFGNLTEVEGQVSGSQVICISPGPKDVPVIPLDQDWFGLELQLRSKETGKIFVSTEFKFYNCSAHQLCLSCVNSAFRCHWCKYRNLCTHDPTTCSFQEGRINVSEDCPQLVPTEEILIPVGEVKPITLKARNLPQPQSGQRGYECVLSIQGAVHRVPALRFNSSSVQCQNSSYQYDGMDISNLAVDFAVVWNGNFIIDNPQDLKVHLYKCAAQRESCGLCLKADHKFECGWCSGERRCTLHQHCPSTSSPWLDWSSHNVKCSNPQITEILTVSGPPEGGTRVTIHGVNLGLDFSEIAHHVQVAGVPCTPIPGEYIIAEQIVCEMGHAVIGTTSGPVRLCIGECKPEFMTKSHQQYTFVNPSVLSLSPIRGPESGGTMVTITGHYLGAGSSVAVYLGNQTCEFYGRSMNEIVCVSPPSSNGLGPVPVSVSVDRARVDSSLQFEYIDDPRVQRIEPEWSITSGHTPLTITGFNLDVIQEPRVRVKFNGKESVNVCTVVNTTTLTCLAPSLTSDYRPGLDTVERPDEFGFLFNNVQSLLIYNDTKFIYYPNPTFELLSPTGILDQKPGSPIILKGKNLCPPASGGAKLNYTVMIGETPCTVTVSETQLLCEPPNLTGQHKVMVHVGGMVFSPGSVSVISDSLLTLPAIISIAAGGSLLLIIVIIVLIAYKRKSRENDLTLKRLQMQMDNLESRVALECKEAFAELQTDINELTSDLDRSGIPYLDYRTYAMRVLFPGIEDHPVLRELEVQGNGQQHVEKALKLFAQLINNKVFLLTFIRTLELQRSFSMRDRGNVASLIMTGLQGRLEYATDVLKQLLSDLIDKNLENKNHPKLLLRRTESVAEKMLTNWFAFLLHKFLKECAGEPLFMLYCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIEYKTLILNCVNPDNENSPEIPVKVLNCDTITQVKEKILDAVYKNVPYSQRPRAVDMDLEWRQGRIARVVLQDEDITTKIEGDWKRLNTLMHYQVSDRSVVALVPKQTSSYNIPASASISRTSISRYDSSFRYTGSPDSLRSRVPMITPDLESGVKVWHLVKNHDHGDQKEGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETLFSTVHRGSALPLAIKYMFDFLDEQADRHSIHDTDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKGSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPSYKNWVERYYADIAKLPAISDQDMNAYLAEQSRLHATEFNMLSALNEIYSYVSKYSEELIGALEQDEQARRQRLAYKVEHLINAMSIES nrp1-plxna2-2 3246338 N/A 3246344 unspecified role unspecified role unspecified role unspecified role Region 21-555 Region 21-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3246345 unspecified role unspecified role unspecified role unspecified role Region 22-586 Region 22-586 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3246346 unspecified role unspecified role unspecified role unspecified role Region 35-703 Region 35-703 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false plxna2-nrp1-1 3246339 N/A 3246345 unspecified role unspecified role prey prey Region 22-586 Region 22-586 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3246346 unspecified role unspecified role bait bait Region 35-703 Region 35-703 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P70207:p.Phe275Asn F275N mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false plxna2-nrp1-2 3246340 N/A 3246345 unspecified role unspecified role prey prey Region 22-586 Region 22-586 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P97333:p.[Pro73Ala;His74Ser] P73A/H74S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3246346 unspecified role unspecified role bait bait Region 35-703 Region 35-703 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false nrp1-sema3a-1 3246341 N/A 3246344 unspecified role unspecified role prey prey Region 21-568 Region 21-568 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3246345 unspecified role unspecified role bait bait Region 22-586 Region 22-586 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P97333:p.[Pro73Ala;His74Ser] P73A/H74S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false sema3a-1 3246342 N/A 3246344 unspecified role unspecified role unspecified role unspecified role Region 21-675 Region 21-675 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O08665:p.[Arg551Ala;Arg555Ala] R551A/R555A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false nrp1-sema3a-2 3246343 N/A 3246344 unspecified role unspecified role prey prey Region 21-568 Region 21-568 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O08665:p.[Leu353Asn;Pro355Ser] L353N/P355S mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3246345 unspecified role unspecified role bait bait Region 22-586 Region 22-586 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false