DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pellegrino-2012-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251328X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions imex curation pellegrino-2012-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251331X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions imex curation pellegrino-2012-3 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251329X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions imex curation pellegrino-2012-4 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251332X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions imex curation pellegrino-2012-5 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251330X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions imex curation pellegrino-2012-6 ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251333X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions imex curation recn_deira DNA repair protein RecN recN Recombination protein N DR_1477 protein protein strain atcc 13939 /-9 Deinococcus radiodurans MTRKARTPKAAPVPEAVAVVEPPPPDAAPTGPRLSRLEIRNLATITQLELELGGGFCAFTGETGAGKSIIVDALGLLLGGRANHDLIRSGEKELLVTGFWGDGDESEADSASRRLSSAGRGAARLSGEVVSVRELQEWAQGRLTIHWQHSAVSLLSPANQRGLLDRRVTKEAQAYAAAHAAWREAVSRLERLQASQRERARQIDLLAFQVQEISEVSPDPGEEEGLNTELSRLSNLHTIAQAAAGGVELLSDGDLNAAGLIGEAVRALNAGAKYDETVMQLQNELRAALESVQAIAGELRDVAEGSAADPEALDRVEARLSALSKLKNKYGPTLEDVVEFGAQAAEELAGLEEDERDAGSLQADVDALHAELLKVGQALDAAREREAEPLVDSLLAVIRELGMPHARMEFALSALAEPAAYGLSDVLLRFSANPGEELGPLSDVASGGELSRVMLAVSTVLGADTPSVVFDEVDAGIGGAAAIAVAEQLSRLADTRQVLVVTHLAQIAARAHHHYKVEKQVEDGRTVSHVRLLTGDERLEEIARMLSGNTSEAALEHARELLAG recn-1 3246124 N/A 3246130 unspecified role unspecified role unspecified role unspecified role Coiled-coil domain Region 196-365 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 196-365 Region 196-365 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 237-291 Region 237-291 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false recn-2 3246125 N/A 3246130 unspecified role unspecified role unspecified role unspecified role Region 196-365 Region 196-365 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false recn-3 3246126 N/A 3246130 unspecified role unspecified role unspecified role unspecified role Coiled-coil domain Region 196-365 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 196-365 Region 196-365 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 237-291 Region 237-291 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false recn-4 3246127 N/A 3246130 unspecified role unspecified role unspecified role unspecified role Region 1-195/366-564 Region 1-195/366-564 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9WXF2:p.[Lys67Ala;Glu472Gln] K67A/E472Q mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false recn-5 3246128 N/A 3246130 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false recn-6 3246129 N/A 3246130 unspecified role unspecified role unspecified role unspecified role Region 1-195/366-564 Region 1-195/366-564 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9WXF2:p.[Lys67Ala;Glu472Gln] K67A/E472Q mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9WXF2:p.[Lys67Ala;Glu472Ala] K67A/E472A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9WXF2:p.Lys67Ala K67A mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9WXF2:p.Glu472Gln E472Q mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9WXF2:p.Glu472Ala E472A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false