DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bao-2012-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Bao X., Nickels BE., Fan H. DIP original experiment: DIP-251803X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Chlamydia trachomatis protein GrgA activates transcription by contacting the nonconserved region of σ66. Bao X., Nickels BE., Fan H. Only protein-protein interactions imex curation bao-2012-2 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Bao X., Nickels BE., Fan H. DIP original experiment: DIP-251805X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Chlamydia trachomatis protein GrgA activates transcription by contacting the nonconserved region of σ66. Bao X., Nickels BE., Fan H. Only protein-protein interactions imex curation bao-2012-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Bao X., Nickels BE., Fan H. DIP original experiment: DIP-251804X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Chlamydia trachomatis protein GrgA activates transcription by contacting the nonconserved region of σ66. Bao X., Nickels BE., Fan H. Only protein-protein interactions imex curation bao-2012-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Bao X., Nickels BE., Fan H. DIP original experiment: DIP-251806X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Chlamydia trachomatis protein GrgA activates transcription by contacting the nonconserved region of σ66. Bao X., Nickels BE., Fan H. Only protein-protein interactions imex curation siga_chltr RNA polymerase sigma factor SigA sigA rpoD CT_615 Sigma-66 Sigma-70 Sigma-A protein protein chltr chltr MRMDTLDSQAAEAAQEEEIQRKLEELVTLAKDQGFITYEEINEILPPSFDTPEQIDQVLIFLAGMDVQVLNQADVERQKERKKEAKELEGLAKRSEGTPDDPVRMYLKEMGTVPLLTREEEVEISKRIEKAQVQIERIILRFRYSTKEAVSIAQYLINGKERFDKIVSEKEVEDKTHFLNLLPKLISLLKEEDAYLEERLLALKDPALSKPDQARLNDELEKCRIRTQAYLRCFHCRHNVTEDFGEVVFKAYDSFLQLEQQINDLKARAERNKFAAAKLAAARRKLHKREVAAGRTLEEFKKDVRMLQRWMDKSQEAKKEMVESNLRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAVTRAIADQARTIRIPVHMIETINKVLRGAKKLMMETGKEPTPEELGEELGFTPDRVREIYKIAQHPISLQAEVGDGGESSFGDFLEDTAVESPAEATGYSMLKDKMKEVLKTLTDRERFVLIHRFGLLDGRPKTLEEVGSAFNVTRERIRQIEAKALRKMRHPIRSKQLRAFLDLLEEEKIGSGKIKSYKN a0a0h3mhh0_chlt2 CTL0766 protein protein chlt2 Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) MYFTRDPVIETVITSREGYKLSIRNSKHLSQDPFVVEAIEVVRLGGTSFFRNCDHSKPFLLPASDYEVMEIRDAKINLKAVGLDRGVKIVGSREALLKMPKVAPIVSVSEDNTIVSEEEVVADSTVAAPASTPVAPMSKKERRKEFKNEKWKDKKKQGRRRNSKEIADAVGSSQEMIDTVAEECLQESSSEEGDFSERRFSLIPPPTRLISDGPEEPEEESQPVTSVDLNESLNALVSESCNVIESILADEDTVVFTKEKDQTAEESQEQPSLSLEETPVHDSISSEE siga-grga-1 3294604 N/A 3294608 unspecified role unspecified role bait bait Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3294609 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false grga-siga-1 3294605 N/A 3294609 unspecified role unspecified role bait bait Strep-tag Strep-tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3294608 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 121-322 Region 121-322 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false grga-siga-2 3294606 N/A 3294609 unspecified role unspecified role bait bait Strep-tag Strep-tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3294608 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 270-316 Region 270-316 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false siga-grga-2 3294607 N/A 3294609 unspecified role unspecified role prey prey Region 1-64 Region 1-64 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3294608 unspecified role unspecified role bait bait Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false