DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yakubovskaya-2012-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yakubovskaya E., Guja KE., Mejia E., Castano S., Hambardjieva E., Choi WS., Garcia-Diaz M. DIP original experiment: DIP-251191X Structure (London, England : 1993) 2012 IMEx Structure of the essential MTERF4:NSUN4 protein complex reveals how an MTERF protein collaborates to facilitate rRNA modification. Yakubovskaya E., Guja KE., Mejia E., Castano S., Hambardjieva E., Choi WS., Garcia-Diaz M. Only protein-protein interactions imex curation yakubovskaya-2012-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yakubovskaya E., Guja KE., Mejia E., Castano S., Hambardjieva E., Choi WS., Garcia-Diaz M. DIP original experiment: DIP-251189X Structure (London, England : 1993) 2012 IMEx Structure of the essential MTERF4:NSUN4 protein complex reveals how an MTERF protein collaborates to facilitate rRNA modification. Yakubovskaya E., Guja KE., Mejia E., Castano S., Hambardjieva E., Choi WS., Garcia-Diaz M. Only protein-protein interactions imex curation yakubovskaya-2012-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Yakubovskaya E., Guja KE., Mejia E., Castano S., Hambardjieva E., Choi WS., Garcia-Diaz M. DIP original experiment: DIP-251190X Structure (London, England : 1993) 2012 IMEx Structure of the essential MTERF4:NSUN4 protein complex reveals how an MTERF protein collaborates to facilitate rRNA modification. Yakubovskaya E., Guja KE., Mejia E., Castano S., Hambardjieva E., Choi WS., Garcia-Diaz M. Only protein-protein interactions imex curation q96cb9-1 5-methylcytosine rRNA methyltransferase NSUN4 NSUN4 NOL1/NOP2/Sun domain family member 4 5-methylcytosine tRNA methyltransferase NSUN4 protein protein human Homo sapiens Human MAALTLRGVRELLKRVDLATVPRRHRYKKKWAATEPKFPAVRLALQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQILPVLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYSIQVQVEDLTHFRRVFMDTFCFFSSCQVGELVIPNLMANFGPMYFCKMRRLT mtef4_human Transcription termination factor 4, mitochondrial Mitochondrial transcription termination factor 4 mTERF domain-containing protein 2 MTERF4 MTERFD2 HSPC096 protein protein human Homo sapiens Human MAAFGRQVLDWHRLIPLTWACMARQTPHLGEQRRTTASLLRKLTTASNGGVIEELSCVRSNNYVQEPECRRNLVQCLLEKQGTPVVQGSLELERVMSSLLDMGFSNAHINELLSVRRGASLQQLLDIISEFILLGLNPEPVCVVLKKSPQLLKLPIMQMRKRSSYLQKLGLGEGKLKRVLYCCPEIFTMRQQDINDTVRLLKEKCLFTVQQVTKILHSCPSVLREDLGQLEYKFQYAYFRMGIKHPDIVKSEYLQYSLTKIKQRHIYLERLGRYQTPDKKGQTQIPNPLLKDILRVSEAEFLARTACTSVEEFQVFKKLLAREEEESESSTSDDKRASLDEDEDDDDEEDNDEDDNDEDDDDEDDDEAEDNDEDEDDDEEE mterf4-nsun4-5 3245363 N/A 3245366 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3245367 unspecified role unspecified role unspecified role unspecified role Region 66-332 Region 66-332 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mterf4-nsun4-6 3245364 N/A 3245366 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3245367 unspecified role unspecified role unspecified role unspecified role Region 66-332 Region 66-332 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mterf4-nsun4-7 3245365 N/A 3245366 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3245367 unspecified role unspecified role unspecified role unspecified role Region 66-332 Region 66-332 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false