DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ martin-2012-2 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252368X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-3 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252371X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-4 ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252373X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-5 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252374X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-6 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252369X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252376X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-8 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252372X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-9 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252370X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-10 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252377X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation martin-2012-11 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. DIP original experiment: DIP-252375X Structure (London, England : 1993) 2012 IMEx The survival motor neuron protein forms soluble glycine zipper oligomers. Martin R., Gupta K., Ninan NS., Perry K., Van Duyne GD. Only protein-protein interactions imex curation gemi2_human Gem-associated protein 2 GEMIN2 SIP1 Component of gems 2 Survival of motor neuron protein-interacting protein 1 protein protein human Homo sapiens Human MRRAELAGLKTMAWVPAESAVEELMPRLLPVEPCDLTEGFDPSVPPRTPQEYLRRVQIEAAQCPDVVVAQIDPKKLKRKQSVNISLSGCQPAPEGYSPTLQWQQQQVAQFSTVRQNVNKHRSHWKSQQLDSNVTMPKSEDEEGWKKFCLGEKLCADGAVGPATNESPGIDYVQIGFPPLLSIVSRMNQATVTSVLEYLSNWFGERDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVDSKDDERVPALNLLICLVSRYFDQRDLADEPS q16637-2 Survival motor neuron protein SMN1 SMN SMNT SMN2 SMNC Iso5-SMN Component of gems 1 Gemin-1 protein protein human Homo sapiens Human MAMSSGGSGGGVPEQEDSVLFRRGTGQSDDSDIWDDTALIKAYDKAVASFKHALKNGDICETSGKPKTTPKRKPAKKNKSQKKNTAASLQQWKVGDKCSAIWSEDGCIYPATIASIDFKRETCVVVYTGYGNREEQNLSDLLSPICEVANNIEQNAQENENESQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKIIPPPPPICPDSLDDADALGSMLISWYMSGYHTGYYMGFRQNQKEGRCSHSLN smn_human Survival motor neuron protein SMN1 SMN SMNT SMN2 SMNC Component of gems 1 Gemin-1 protein protein human Homo sapiens Human MAMSSGGSGGGVPEQEDSVLFRRGTGQSDDSDIWDDTALIKAYDKAVASFKHALKNGDICETSGKPKTTPKRKPAKKNKSQKKNTAASLQQWKVGDKCSAIWSEDGCIYPATIASIDFKRETCVVVYTGYGNREEQNLSDLLSPICEVANNIEQNAQENENESQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDADALGSMLISWYMSGYHTGYYMGFRQNQKEGRCSHSLN gemin2-smn1-5 3245276 N/A 3245286 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3245287 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gemin2-smn1-6 3245277 N/A 3245286 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3245287 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false smn1-1 3245278 N/A 3245288 unspecified role unspecified role unspecified role unspecified role Region 263-294 Region 263-294 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false smn1-3 3245279 N/A 3245288 unspecified role unspecified role unspecified role unspecified role Region 263-294 Region 263-294 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false gemin2-smn1-7 3245280 N/A 3245286 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3245287 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false smn1-4 3245281 N/A 3245288 unspecified role unspecified role unspecified role unspecified role MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 263-294 Region 263-294 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smn1-5 3245282 N/A 3245288 unspecified role unspecified role unspecified role unspecified role Region 263-294 Region 263-294 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q16637:p.Gly279Val G279V mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Gly275Ser G275S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr272Cys Y272C mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Ser266Pro S266P mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met263Arg M263R/T mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Leu260Ser L260S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Thr274Ala T274A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Gly271Ala G271A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Gly275Ala G275A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr268Ala Y268A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Trp267Ala W267A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met263Ala M263A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Leu264Ala L264A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr272Ala Y272A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Thr274Ile T274I mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr276Ala Y276A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr277Ala Y277A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met269Ala M269A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met263Thr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false gemin2-smn1-8 3245283 N/A 3245286 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3245287 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false smn1-6 3245284 N/A 3245288 unspecified role unspecified role unspecified role unspecified role Region 263-294 Region 263-294 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q16637:p.Gly279Val G279V mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Gly275Ser G275S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr272Cys Y272C mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Ser266Pro S266P mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met263Arg M263R/T mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Leu260Ser L260S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Thr274Ala T274A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Gly271Ala G271A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Gly275Ala G275A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr268Ala Y268A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Trp267Ala W267A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met263Ala M263A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Leu264Ala L264A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr272Ala Y272A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Thr274Ile T274I mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr276Ala Y276A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Tyr277Ala Y277A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met269Ala M269A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q16637:p.Met263Thr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smn1-7 3245285 N/A 3245288 unspecified role unspecified role unspecified role unspecified role Region 263-294 Region 263-294 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false