DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sphr-2012-1 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251789X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions imex curation sphr-2012-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251793X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions imex curation sphr-2012-3 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251791X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions imex curation sphr-2012-4 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251790X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions imex curation sphr-2012-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251792X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions imex curation q96cb9-1 5-methylcytosine rRNA methyltransferase NSUN4 NSUN4 NOL1/NOP2/Sun domain family member 4 5-methylcytosine tRNA methyltransferase NSUN4 protein protein human Homo sapiens Human MAALTLRGVRELLKRVDLATVPRRHRYKKKWAATEPKFPAVRLALQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQILPVLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYSIQVQVEDLTHFRRVFMDTFCFFSSCQVGELVIPNLMANFGPMYFCKMRRLT mtef4_human Transcription termination factor 4, mitochondrial Mitochondrial transcription termination factor 4 mTERF domain-containing protein 2 MTERF4 MTERFD2 HSPC096 protein protein human Homo sapiens Human MAAFGRQVLDWHRLIPLTWACMARQTPHLGEQRRTTASLLRKLTTASNGGVIEELSCVRSNNYVQEPECRRNLVQCLLEKQGTPVVQGSLELERVMSSLLDMGFSNAHINELLSVRRGASLQQLLDIISEFILLGLNPEPVCVVLKKSPQLLKLPIMQMRKRSSYLQKLGLGEGKLKRVLYCCPEIFTMRQQDINDTVRLLKEKCLFTVQQVTKILHSCPSVLREDLGQLEYKFQYAYFRMGIKHPDIVKSEYLQYSLTKIKQRHIYLERLGRYQTPDKKGQTQIPNPLLKDILRVSEAEFLARTACTSVEEFQVFKKLLAREEEESESSTSDDKRASLDEDEDDDDEEDNDEDDNDEDDDDEDDDEAEDNDEDEDDDEEE mterf4-nsun4-1 3244849 N/A 3244854 unspecified role unspecified role prey prey Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244855 unspecified role unspecified role bait bait Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false nsun4-1 3244850 N/A 3244854 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q96CB9-1:p.[Val65Arg;Arg136Ala;Ile139Arg;Arg141Ala] V65R/I139R/R136A/R141A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mterf4-nsun4-2 3244851 N/A 3244854 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244855 unspecified role unspecified role unspecified role unspecified role Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mterf4-nsun4-3 3244852 N/A 3244854 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244855 unspecified role unspecified role unspecified role unspecified role Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mterf4-nsun4-4 3244853 N/A 3244854 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q96CB9-1:p.[Val65Arg;Arg136Ala;Ile139Arg;Arg141Ala] V65R/I139R/R136A/R141A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244855 unspecified role unspecified role unspecified role unspecified role Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false