DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ o_connell-2012-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251016X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251014X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251023X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-4 in vitro In vitro pull down pull down predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251018X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-5 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251021X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251017X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-7 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251015X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-8 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251019X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-9 in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251024X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-10 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251020X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation o_connell-2012-11 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. DIP original experiment: DIP-251022X Structure (London, England : 1993) 2012 IMEx The molecular basis for substrate specificity of the nuclear NIPP1:PP1 holoenzyme. O'Connell N., Nichols SR., Heroes E., Beullens M., Bollen M., Peti W., Page R. Only protein-protein interactions imex curation q12972-1 Nuclear inhibitor of protein phosphatase 1 NIPP1 Protein phosphatase 1 regulatory inhibitor subunit 8 PPP1R8 ARD1 protein protein human Homo sapiens Human MAAAANSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI pp1a_human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit PPP1CA PPP1A protein protein human Homo sapiens Human MSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKNKGKYGQFSGLNPGGRPITPPRNSAKAKK cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL ccna2_bovin Cyclin-A2 CCNA2 protein protein bovin Bos taurus (Bovine) Bovine MLGSSAHGPAAREAGSAVTLQQTAFQEDQENVNPEKAAPAQQPRTRAGLAVLRAGNSRGPAPQRPKTRRVAPLKDLPINDEYVPVPPWKANNKQPAFTIHVDEAEEEIQKRPTESKKSESEDVLAFNSAVTLPGPRKPLAPLDYPMDGSFESPHTMEMSVVLEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLAFDLAAPTINQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAAAAFHLALYTVTGQSWPESLVQKTGYTLETLKPCLLDLHQTYLRAPQHAQQSIREKYKNSKYHGVSLLNPPETLNV o75533-1 Splicing factor 3B subunit 1 SF3B1 SAP155 Pre-mRNA-splicing factor SF3b 155 kDa subunit Spliceosome-associated protein 155 protein protein human Homo sapiens Human MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL cdc5l_human Cell division cycle 5-like protein CDC5L Pombe cdc5-related protein KIAA0432 PCDC5RP protein protein human Homo sapiens Human MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF pygm_rabit Glycogen phosphorylase, muscle form Myophosphorylase PYGM protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MSRPLSDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRVLVDLERLDWDKAWEVTVKTCAYTNHTVLPEALERWPVHLLETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEIIAERIGEEYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHVITLYNRIKKEPNKFVVPRTVMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPVVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDRLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYVKCQERVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKIP ppp1ca-ppp1r8-5 3244686 N/A 3244697 unspecified role unspecified role unspecified role unspecified role Region 144-225 Region 144-225 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 unspecified role unspecified role unspecified role unspecified role Region 7-330 Region 7-330 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ppp1ca-ppp1r8-6 3244687 N/A 3244697 unspecified role unspecified role unspecified role unspecified role Region 158-216 Region 158-216 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 unspecified role unspecified role unspecified role unspecified role Region 7-330 Region 7-330 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ppp1ca-ppp1r8-7 3244688 N/A 3244697 unspecified role unspecified role prey prey Region 158-216 Region 158-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 unspecified role unspecified role bait bait Region 7-307 Region 7-307 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ccna2-cdk2-15 3244689 N/A 3244699 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3244700 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ppp1ca-sf3b1-1 3244690 N/A 3244701 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-491 Region 1-491 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 enzyme enzyme unspecified role unspecified role Region 7-330 Region 7-330 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false ppp1ca-ppp1r8-8 3244691 N/A 3244697 unspecified role unspecified role unspecified role unspecified role Region 158-216 Region 158-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 unspecified role unspecified role unspecified role unspecified role Region 7-307 Region 7-307 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ppp1ca-ppp1r8-9 3244692 N/A 3244697 unspecified role unspecified role unspecified role unspecified role Region 158-216 Region 158-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 unspecified role unspecified role unspecified role unspecified role Region 7-307 Region 7-307 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ccna2-cdk2-16 3244693 N/A 3244699 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3244700 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ppp1ca-ppp1r8-10 3244694 N/A 3244697 unspecified role unspecified role unspecified role unspecified role Region 158-216 Region 158-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 unspecified role unspecified role unspecified role unspecified role Region 7-307 Region 7-307 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdc5l-ppp1ca-1 3244695 N/A 3244702 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 260-606 Region 260-606 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3244698 enzyme enzyme unspecified role unspecified role Region 7-330 Region 7-330 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false ppp1ca-pygm-1 3244696 N/A 3244703 enzyme target enzyme target substrate substrate unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3244698 enzyme enzyme unspecified role unspecified role Region 7-330 Region 7-330 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false