DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dou-2012-7 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251156X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-8 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251144X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-9 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251152X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-10 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251159X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-11 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251158X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-12 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251145X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-13 in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251148X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-14 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251153X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-15 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251149X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-16 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251146X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-17 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251150X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-18 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251157X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-19 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251155X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-20 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251154X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-21 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251151X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation dou-2012-22 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. DIP original experiment: DIP-251147X Nature structural & molecular biology 2012 IMEx BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer. Dou H., Buetow L., Sibbet GJ., Cameron K., Huang DT. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ub2d2_human Ubiquitin-conjugating enzyme E2 D2 UBC5B UBCH4 UBE2D2 UBC4 UBCH5B Ubiquitin-protein ligase D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-conjugating enzyme E2(17)KB 2 PUBC1 p53-regulated ubiquitin-conjugating enzyme 1 (E3-independent) E2 ubiquitin-conjugating enzyme D2 E2 ubiquitin-conjugating enzyme D2 protein protein human Homo sapiens Human MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM birc3_human Baculoviral IAP repeat-containing protein 3 Inhibitor of apoptosis protein 1 TNFR2-TRAF-signaling complex protein 1 IAP homolog C Apoptosis inhibitor 2 RING finger protein 49 BIRC3 API2 MIHC RNF49 RING-type E3 ubiquitin transferase BIRC3 Cellular inhibitor of apoptosis 2 protein protein human Homo sapiens Human MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS mdm4_human Protein Mdm4 MDM4 MDMX p53-binding protein Mdm4 Mdm2-like p53-binding protein Protein Mdmx Double minute 4 protein protein protein human Homo sapiens Human MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP q96ca5-1 Baculoviral IAP repeat-containing protein 7 BIRC7 KIAP LIVIN MLIAP RNF50 UNQ5800/PRO19607/PRO21344 Livin alpha Kidney inhibitor of apoptosis protein Livin Melanoma inhibitor of apoptosis protein RING finger protein 50 RING-type E3 ubiquitin transferase BIRC7 protein protein human Homo sapiens Human MGPKDSAKCLHRGPQPSHWAAGDGPTQERCGPRSLGSPVLGLDTCRAWDHVDGQILGQLRPLTEEEEEEGAGATLSRGPAFPGMGSEELRLASFYDWPLTAEVPPELLAAAGFFHTGHQDKVRCFFCYGGLQSWKRGDDPWTEHAKWFPSCQFLLRSKGRDFVHSVQETHSQLLGSWDPWEEPEDAAPVAPSVPASGYPELPTPRREVQSESAQEPGGVSPAEAQRAWWVLEPPGARDVEAQLRRLQEERTCKVCLDRAVSIVFVPCGHLVCAECAPGLQLCPICRAPVRSRVRTFLS bmi1_human Polycomb complex protein BMI-1 BMI1 PCGF4 RNF51 Polycomb group RING finger protein 4 RING finger protein 51 protein protein human Homo sapiens Human MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG ring2_human E3 ubiquitin-protein ligase RING2 RING finger protein 2 RING finger protein 1B RING finger protein BAP-1 Protein DinG Huntingtin-interacting protein 2-interacting protein 3 RNF2 BAP1 DING HIPI3 RING1B RING-type E3 ubiquitin transferase RING2 protein protein human Homo sapiens Human MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK birc3-ub-1 3243670 N/A 3243686 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3243687 unspecified role unspecified role prey prey P62837:p.Cys85Ser C85S mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3243688 unspecified role unspecified role bait bait Region 541-604 Region 541-604 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false mdm2-mdm4-10 3243671 N/A 3243689 unspecified role unspecified role prey prey Region 428-490 Region 428-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3243690 unspecified role unspecified role bait bait Region 428-491 Region 428-491 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false birc7-ub-1 3243672 N/A 3243691 unspecified role unspecified role unspecified role unspecified role Region 239-298 Region 239-298 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3243686 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3243687 unspecified role unspecified role unspecified role unspecified role P62837:p.[Ser22Arg;Asn77Ala;Cys85Ser] C85S/S22R/N77A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false birc7-ub-2 3243673 N/A 3243691 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3243686 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3243687 unspecified role unspecified role prey prey P62837:p.Cys85Ser C85S mutation mutation certain certain sequence position certain certain certain sequence position certain false P62837:p.Ile88Ala I88A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P62837:p.Ser108Arg S108R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false birc7-ub-3 3243674 N/A 3243691 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3243686 unspecified role unspecified role prey prey L8D L8D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G35E G35E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false I36A I36A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false I44A I44A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3243687 unspecified role unspecified role prey prey P62837:p.Cys85Ser C85S mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false mdm4-mdm2-3 3243675 N/A 3243689 unspecified role unspecified role bait bait Region 428-490 Region 428-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3243690 unspecified role unspecified role prey prey Region 428-491 Region 428-491 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ub-ube2d2-6 3243676 N/A 3243686 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3243687 unspecified role unspecified role unspecified role unspecified role P62837:p.Cys85Ser C85S mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc7-ub-4 3243677 N/A 3243691 unspecified role unspecified role unspecified role unspecified role Region 239-298 Region 239-298 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3243686 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3243687 unspecified role unspecified role unspecified role unspecified role P62837:p.[Ser22Arg;Asn77Ala;Cys85Ser] C85S/S22R/N77A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2d2-7 3243678 N/A 3243686 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3243687 unspecified role unspecified role unspecified role unspecified role P62837:p.Cys85Ser C85S mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rnf2-bmi1-5 3243679 N/A 3243692 unspecified role unspecified role prey prey Region 1-109 Region 1-109 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3243693 unspecified role unspecified role bait bait Region 1-114 Region 1-114 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ub-ube2d2-8 3243680 N/A 3243686 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3243687 unspecified role unspecified role unspecified role unspecified role P62837:p.[Ser22Arg;Asn77Arg;Cys85Ser] C85S/S22R/N77R mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc7-ub-5 3243681 N/A 3243691 unspecified role unspecified role bait bait Region 239-298 Region 239-298 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q96CA5-1:p.Val263Arg V263R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q96CA5-1:p.Phe296_Ser298del Δ296-298 mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q96CA5-1:p.Val254Ala V254A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96CA5-1:p.Ile284Ala I284A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96CA5-1:p.Arg286Ala R286A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96CA5-1:p.Phe296His F296H mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3243686 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3243687 unspecified role unspecified role prey prey P62837:p.Cys85Ser C85S mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false birc3-ube2d2-1 3243682 N/A 3243687 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3243688 unspecified role unspecified role bait bait Region 541-604 Region 541-604 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc7-1 3243683 N/A 3243691 unspecified role unspecified role unspecified role unspecified role Q96CA5-1:p.Val263Arg V263R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q96CA5-1:p.Phe296_Ser298del Δ296-298 mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2d2-9 3243684 N/A 3243686 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3243687 unspecified role unspecified role unspecified role unspecified role P62837:p.Cys85Ser C85S mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bmi1-rnf2-9 3243685 N/A 3243692 unspecified role unspecified role bait bait Region 1-109 Region 1-109 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3243693 unspecified role unspecified role prey prey Region 1-114 Region 1-114 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false