DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ plechanovov-2012-1 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Plechanovová A., Jaffray EG., Tatham MH., Naismith JH., Hay RT. DIP original experiment: DIP-250698X Nature 2012 IMEx Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis. Plechanovová A., Jaffray EG., Tatham MH., Naismith JH., Hay RT. Only protein-protein interactions imex curation plechanovov-2012-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Plechanovová A., Jaffray EG., Tatham MH., Naismith JH., Hay RT. DIP original experiment: DIP-250699X Nature 2012 IMEx Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis. Plechanovová A., Jaffray EG., Tatham MH., Naismith JH., Hay RT. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ub2d1_human Ubiquitin-conjugating enzyme E2 D1 Ubiquitin-protein ligase D1 Ubiquitin carrier protein D1 UbcH5 Ubiquitin-conjugating enzyme E2-17 kDa 1 Ubiquitin-conjugating enzyme E2(17)KB 1 UBC4/5 homolog Stimulator of Fe transport UBE2D1 UBCH5 UBCH5A SFT UBC5A (E3-independent) E2 ubiquitin-conjugating enzyme D1 E2 ubiquitin-conjugating enzyme D1 protein protein human Homo sapiens Human MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM ub-ube2d1-1 3243092 N/A 3243094 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3243095 unspecified role unspecified role unspecified role unspecified role P51668:p.Arg90Ala R90A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2d1-2 3243093 N/A 3243094 unspecified role unspecified role unspecified role unspecified role arg72ala R72A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3243095 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false