DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ biter-2012-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Biter AB., Lee S., Sung N., Tsai FT. DIP original experiment: DIP-251732X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis for intersubunit signaling in a protein disaggregating machine. Biter AB., Lee S., Sung N., Tsai FT. Only protein-protein interactions imex curation biter-2012-2 ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Biter AB., Lee S., Sung N., Tsai FT. DIP original experiment: DIP-251736X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis for intersubunit signaling in a protein disaggregating machine. Biter AB., Lee S., Sung N., Tsai FT. Only protein-protein interactions imex curation biter-2012-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Biter AB., Lee S., Sung N., Tsai FT. DIP original experiment: DIP-251733X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis for intersubunit signaling in a protein disaggregating machine. Biter AB., Lee S., Sung N., Tsai FT. Only protein-protein interactions imex curation biter-2012-4 ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Biter AB., Lee S., Sung N., Tsai FT. DIP original experiment: DIP-251734X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis for intersubunit signaling in a protein disaggregating machine. Biter AB., Lee S., Sung N., Tsai FT. Only protein-protein interactions imex curation biter-2012-5 ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Biter AB., Lee S., Sung N., Tsai FT. DIP original experiment: DIP-251735X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis for intersubunit signaling in a protein disaggregating machine. Biter AB., Lee S., Sung N., Tsai FT. Only protein-protein interactions imex curation clpb_thet8 Chaperone protein ClpB clpB TTHA1487 protein protein thet8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) MNLERWTQAAREALAQAQVLAQRMKHQAIDLPHLWAVLLKDERSLAWRLLEKAGADPKALKELQERELARLPKVEGAEVGQYLTSRLSGALNRAEALMEELKDRYVAVDTLVLALAEATPGLPGLEALKGALKELRGGRTVQTEHAESTYNALEQYGIDLTRLAAEGKLDPVIGRDEEIRRVIQILLRRTKNNPVLIGEPGVGKTAIVEGLAQRIVKGDVPEGLKGKRIVSLQMGSLLAGAKYRGEFEERLKAVIQEVVQSQGEVILFIDELHTVVGAGKAEGAVDAGNMLKPALARGELRLIGATTLDEYREIEKDPALERRFQPVYVDEPTVEETISILRGLKEKYEVHHGVRISDSAIIAAATLSHRYITERRLPDKAIDLIDEAAARLRMALESAPEEIDALERKKLQLEIEREALKKEKDPDSQERLKAIEAEIAKLTEEIAKLRAEWEREREILRKLREAQHRLDEVRREIELAERQYDLNRAAELRYGELPKLEAEVEALSEKLRGARFVRLEVTEEDIAEIVSRWTGIPVSKLLEGEREKLLRLEEELHKRVVGQDEAIRAVADAIRRARAGLKDPNRPIGSFLFLGPTGVGKTELAKTLAATLFDTEEAMIRIDMTEYMEKHAVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVILFDEIEKAHPDVFNILLQILDDGRLTDSHGRTVDFRNTVIILTSNLGSPLILEGLQKGWPYERIRDEVFKVLQQHFRPEFLNRLDEIVVFRPLTKEQIRQIVEIQLSYLRARLAEKRISLELTEAAKDFLAERGYDPVFGARPLRRVIQRELETPLAQKILAGEVKEGDRVQVDVGPAGLVFAVPARVEA mint Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with dnaK, dnaJ and grpE. Acts before dnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of clpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by dnaK clpb-6 3242730 N/A 3242735 unspecified role unspecified role unspecified role unspecified role Region 542-854 Region 542-854 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clpb-7 3242731 N/A 3242735 unspecified role unspecified role unspecified role unspecified role Q9RA63:p.[Asn746Cys;Arg810Cys] N746C/R810C mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clpb-8 3242732 N/A 3242735 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false clpb-9 3242733 N/A 3242735 unspecified role unspecified role unspecified role unspecified role Q9RA63:p.[Arg576Cys;Ala821Cys] R576C/A821C mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clpb-10 3242734 N/A 3242735 unspecified role unspecified role unspecified role unspecified role Q9RA63:p.[Leu581Cys;Arg776Cys] L581C/R776C mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false