DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lu-2012-10 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254589X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-11 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254584X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-12 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254594X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-13 mouse Mus musculus Mouse molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254585X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-14 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254595X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-15 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254590X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-16 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254586X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-17 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254591X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-18 mouse Mus musculus Mouse molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254596X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-19 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254587X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-20 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254592X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-21 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254593X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation lu-2012-22 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. DIP original experiment: DIP-254588X Nature 2012 IMEx Novel role of PKR in inflammasome activation and HMGB1 release. Lu B., Nakamura T., Inouye K., Li J., Tang Y., Lundbäck P., Valdes-Ferrer SI., Olofsson PS., Kalb T., Roth J., Zou Y., Erlandsson-Harris H., Yang H., Ting JP., Wang H., Andersson U., Antoine DJ., Chavan SS., Hotamisligil GS., Tracey KJ. Only protein-protein interactions imex curation aim2_human Interferon-inducible protein AIM2 AIM2 Absent in melanoma 2 protein protein human Homo sapiens Human MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKT e2ak2_human Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 Protein kinase RNA-activated p68 kinase P1/eIF-2A protein kinase EIF2AK2 PKR PRKR Tyrosine-protein kinase EIF2AK2 protein protein human Homo sapiens Human MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC nlrp3_mouse NACHT, LRR and PYD domains-containing protein 3 Nlrp3 Cias1 Mmig1 Nalp3 Pypaf1 Cold autoinflammatory syndrome 1 protein homolog Cryopyrin Mast cell maturation-associated-inducible protein 1 PYRIN-containing APAF1-like protein 1 protein protein mouse Mus musculus Mouse MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW e2ak2_mouse Interferon-induced, double-stranded RNA-activated protein kinase Eif2ak2 Pkr Prkr Tik Interferon-inducible RNA-dependent protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 Protein kinase RNA-activated p68 kinase P1/eIF-2A protein kinase Serine/threonine-protein kinase TIK Tyrosine-protein kinase EIF2AK2 protein protein mouse Mus musculus Mouse MASDTPGFYMDKLNKYRQMHGVAITYKELSTSGPPHDRRFTFQVLIDEKEFPEAKGRSKQEARNAAAKLAVDILDNENKVDCHTSASEQGLFVGNYIGLVNSFAQKKKLSVNYEQCEPNSELPQRFICKCKIGQTMYGTGSGVTKQEAKQLAAKEAYQKLLKSPPKTAGTSSSVVTSTFSGFSSSSSMTSNGVSQSAPGSFSSENVFTNGLGENKRKSGVKVSPDDVQRNKYTLDARFNSDFEDIEEIGLGGFGQVFKAKHRIDGKRYAIKRVKYNTEKAEHEVQALAELNHVNIVQYHSCWEGVDYDPEHSMSDTSRYKTRCLFIQMEFCDKGTLEQWMRNRNQSKVDKALILDLYEQIVTGVEYIHSKGLIHRDLKPGNIFLVDERHIKIGDFGLATALENDGKSRTRRTGTLQYMSPEQLFLKHYGKEVDIFALGLILAELLHTCFTESEKIKFFESLRKGDFSNDIFDNKEKSLLKKLLSEKPKDRPETSEILKTLAEWRNISEKKKRNTC nlrp3_human NACHT, LRR and PYD domains-containing protein 3 NLRP3 C1orf7 CIAS1 NALP3 PYPAF1 Angiotensin/vasopressin receptor AII/AVP-like Caterpiller protein 1.1 Cryopyrin PYRIN-containing APAF1-like protein 1 Cold-induced autoinflammatory syndrome 1 protein protein protein human Homo sapiens Human MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW casp1_mouse Caspase-1 Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 Casp1 Il1bc protein protein mouse Mus musculus Mouse MADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKLANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATEDSKGGHPSSSETKEEQNKEDGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRTRLALIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAACPEHKTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIIIQACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFYLFPGH asc_mouse Apoptosis-associated speck-like protein containing a CARD Pycard Asc PYD and CARD domain-containing protein protein protein mouse Mus musculus Mouse MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS nlrp1_human NACHT, LRR and PYD domains-containing protein 1 NLRP1 CARD7 DEFCAP KIAA0926 NAC NALP1 Death effector filament-forming ced-4-like apoptosis protein Nucleotide-binding domain and caspase recruitment domain Caspase recruitment domain-containing protein 7 protein protein human Homo sapiens Human MAGGAWGRLACYLEFLKKEELKEFQLLLANKAHSRSSSGETPAQPEKTSGMEVASYLVAQYGEQRAWDLALHTWEQMGLRSLCAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTAVLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWREISASLLYQALPSSPDHESPSQESPNAPTSTAVLGSWGSPPQPSLAPREQEAPGTQWPLDETSGIYYTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQPHHHPWEPSVRESLCSTWPWKNEDFNQKFTQLLLLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIRDLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKEAWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAELIGKDGTATPAPIRQILSRPERLLFILDGVDEPGWVLQEPSSELCLHWSQPQPADALLGSLLGKTILPEASFLITARTTALQNLIPSLEQARWVEVLGFSESSRKEYFYRYFTDERQAIRAFRLVKSNKELWALCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTTLCLHYLAQALQAQPLGPQLRDLCSLAAEGIWQKKTLFSPDDLRKHGLDGAIISTFLKMGILQEHPIPLSYSFIHLCFQEFFAAMSYVLEDEKGRGKHSNCIIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEGEREMENIFHCRLSQGRNLMQWVPSLQLLLQPHSLESLHCLYETRNKTFLTQVMAHFEEMGMCVETDMELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPTMVVLFRWVPVTDAYWQILFSVLKVTRNLKELDLSGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGLTAEDCKDLAFGLRANQTLTELDLSFNVLTDAGAKHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDLASVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHPACKLIRLGLDQTTLSDEMRQELRALEQEKPQLLIFSRRKPSVMTPTEGLDTGEMSNSTSSLKRQRLGSERAASHVAQANLKLLDVSKIFPIAEIAEESSPEVVPVELLCVPSPASQGDLHTKPLGTDDDFWGPTGPVATEVVDKEKNLYRVHFPVAGSYRWPNTGLCFVMREAVTVEIEFCVWDQFLGEINPQHSWMVAGPLLDIKAEPGAVEAVHLPHFVALQGGHVDTSLFQMAHFKEEGMLLEKPARVELHHIVLENPSFSPLGVLLKMIHNALRFIPVTSVVLLYHRVHPEEVTFHLYLIPSDCSIRKAIDDLEMKFQFVRIHKPPPLTPLYMGCRYTVSGSGSGMLEILPKELELCYRSPGEDQLFSEFYVGHLGSGIRLQVKDKKDETLVWEALVKPGDLMPATTLIPPARIAVPSPLDAPQLLHFVDQYREQLIARVTSVEVVLDKLHGQVLSQEQYERVLAENTRPSQMRKLFSLSQSWDRKCKDGLYQALKETHPHLIMELWEKGSKKGLLPLSS q9npp4-1 NLR family CARD domain-containing protein 4 NLRC4 CARD12 CLAN CLAN1 IPAF CARD, LRR, and NACHT-containing protein Caspase recruitment domain-containing protein 12 Ice protease-activating factor UNQ6189/PRO20215 CLANA protein protein human Homo sapiens Human MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILKKGSESCNLFLKSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPLGEDIDIIFNLKSTFTEPVLWRKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQRIAMLWGSGKCKALTKFKFVFFLRLSRAQGGLFETLCDQLLDIPGTIRKQTFMAMLLKLRQRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRQFGALTAEVGDMTEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMGESEFHSHTQTTLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDVLLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSISDITSTYSSLLRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNTTEQEILKAININSFVECGIHLYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFEHLPNCASALDFIKLDFYGGAMASWEKAAEDTGGIHMEEAPETYIPSRAVSLFFNWKQEFRTLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGVAGSLSLVLSTCKNIYSLMVEASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIMDNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLLKHLEEVPQLVKLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPDPALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFKLVTA aim2-eif2ak2-1 3242518 N/A 3242531 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3242532 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false eif2ak2-nlrp3-1 3242519 N/A 3242533 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3242534 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false nlrp3-eif2ak2-1 3242520 N/A 3242535 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3242532 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false eif2ak2-nlrp3-2 3242521 N/A 3242533 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse N/A 3242534 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse physical association physical association false false false eif2ak2-nlrp3-3 3242522 N/A 3242535 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3242532 unspecified role unspecified role bait bait P19525:p.Lys296Arg K296R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false nlrp3-eif2ak2-2 3242523 N/A 3242533 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3242534 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false eif2ak2-nlrp3-4 3242524 N/A 3242535 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3242532 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse direct interaction direct interaction false false false eif2ak2-nlrp3-5 3242525 N/A 3242535 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3242532 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false casp1-eif2ak2-1 3242526 N/A 3242536 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse N/A 3242537 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse N/A 3242533 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse N/A 3242534 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse physical association physical association false false false nlrp1-eif2ak2-1 3242527 N/A 3242538 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3242532 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false nlrp3-eif2ak2-3 3242528 N/A 3242535 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3242532 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false nlrp3-eif2ak2-4 3242529 N/A 3242535 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false PYD domain Region 1-217 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false NACHT domain Region 218-532 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false LRR domain Region 533-1036 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3242532 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false nlrc4-eif2ak2-1 3242530 N/A 3242539 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3242532 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false