DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ames-2012-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Ames BD., Nguyen C., Bruegger J., Smith P., Xu W., Ma S., Wong E., Wong S., Xie X., Li JW., Vederas JC., Tang Y., Tsai SC. DIP original experiment: DIP-251676X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Crystal structure and biochemical studies of the trans-acting polyketide enoyl reductase LovC from lovastatin biosynthesis. Ames BD., Nguyen C., Bruegger J., Smith P., Xu W., Ma S., Wong E., Wong S., Xie X., Li JW., Vederas JC., Tang Y., Tsai SC. Only protein-protein interactions imex curation lovc_aspte Lovastatin nonaketide synthase, enoyl reductase component lovC lovC Lovastatin biosynthesis cluster protein C Trans-enoyl reductase lovC protein protein aspte Aspergillus terreus MGDQPFIPPPQQTALTVNDHDEVTVWNAAPCPMLPRDQVYVRVEAVAINPSDTKMRGQFATPWAFLGTDYAGTVVAVGSDVTHIQVGDRVYGAQNEMCPRTPDQGAFSQYTVTRGRVWAKIPKGLSFEQAAALPAGISTAGLAMKLLGLPLPSPSADQPPTHSKPVYVLVYGGSTATATVTMQMLRLSGYIPIATCSPHNFDLAKSRGAEEVFDYRAPNLAQTIRTYTKNNLRYALDCITNVESTTFCFAAIGRAGGHYVSLNPFPEHAATRKMVTTDWTLGPTIFGEGSTWPAPYGRPGSEEERQFGEDLWRIAGQLVEDGRLVHHPLRVVQGGFDHIKQGMELVRKGELSGEKLVVRLEGP lovb_aspte Lovastatin nonaketide synthase, polyketide synthase component lovB Lovastatin biosynthesis cluster protein B protein protein aspte Aspergillus terreus MAQSMYPNEPIVVVGSGCRFPGDANTPSKLWELLQHPRDVQSRIPKERFDVDTFYHPDGKHHGRTNAPYAYVLQDDLGAFDAAFFNIQAGEAESMDPQHRLLLETVYEAVTNAGMRIQDLQGTSTAVYVGVMTHDYETVSTRDLESIPTYSATGVAVSVASNRISYFFDWHGPSMTIDTACSSSLVAVHLAVQQLRTGQSSMAIAAGANLILGPMTFVLESKLSMLSPSGRSRMWDAGADGYARGEAVCSVVLKTLSQALRDGDTIECVIRETGVNQDGRTTGITMPNHSAQEALIKATYAQAGLDITKAEDRCQFFEAHGTGTPAGDPQEAEAIATAFFGHEQVARSDGNERAPLFVGSAKTVVGHTEGTAGLAGLMKASFAVRHGVIPPNLLFDKISPRVAPFYKNLRIPTEATQWPALPPGQPRRASVNSFGFGGTNAHAIIEEYMEPEQNQLRVSNNEDCPPMTGVLSLPLVLSAKSQRSLKIMMEEMLQFLQSHPEIHLHDLTWSLLRKRSVLPFRRAIVGHSHETIRRALEDAIEDGIVSSDFTTEVRGQPSVLGIFTGQGAQWPGMLKNLIEASPYVRNIVRELDDSLQSLPEKYRPSWTLLDQFMLEGEASNVQYATFSQPLCCAVQIVLVRLLEAARIRFTAVVGHSSGEIACAFAAGLISASLAIRIAYLRGVVSAGGARGTPGAMLAAGMSFEEAQEICELDAFEGRICVAASNSPDSVTFSGDANAIDHLKGMLEDESTFARLLKVDTAYHSHHMLPCADPYMQALEECGCAVADAGSPAGSVPWYSSVDAENRQMAARDVTAKYWKDNLVSPVLFSHAVQRAVVTHKALDIGIEVGCHPALKSPCVATIKDVLSGVDLAYTGCLERGKNDLDSFSRALAYLWERFGASSFDADEFMRAVAPDRPCMSVSKLLPAYPWDRSRRYWVESRATRHHLRGPKPHLLLGKLSEYSTPLSFQWLNFVRPRDIEWLDGHALQGQTVFPAAGYIVMAMEAALMIAGTHAKQVKLLEILDMSIDKAVIFDDEDSLVELNLTADVSRNAGEAGSMTISFKIDSCLSKEGNLSLSAKGQLALTIEDVNPRTTSASDQHHLPPPEEEHPHMNRVNINAFYHELGLMGYNYSKDFRRLHNMQRADLRASGTLDFIPLMDEGNGCPLLLHPASLDVAFQTVIGAYSSPGDRRLRCLYVPTHVDRITLVPSLCLATAESGCEKVAFNTINTYDKGDYLSGDIVVFDAEQTTLFQVENITFKPFSPPDASTDHAMFARWSWGPLTPDSLLDNPEYWATAQDKEAIPIIERIVYFYIRSFLSQLTLEERQQAAFHLQKQIEWLEQVLASAKEGRHLWYDPGWENDTEAQIEHLCTANSYHPHVRLVQRVGQHLLPTVRSNGNPFDLLDHDGLLTEFYTNTLSFGPALHYARELVAQIAHRYQSMDILEIGAGTGGATKYVLATPQLGFNSYTYTDISTGFFEQAREQFAPFEDRMVFEPLDIRRSPAEQGFEPHAYDLIIASNVLHATPDLEKTMAHARSLLKPGGQMVILEITHKEHTRLGFIFGLFADWWAGVDDGRCTEPFVSFDRWDAILKRVGFSGVDSRTTDRDANLFPTSVFSTHAIDATVEYLDAPLASSGTVKDSYPPLVVVGGQTPQSQRLLNDIKAIMPPRPLQTYKRLVDLLDAEELPMKSTFVMLTELDEELFAGLTEETFEATKLLLTYASNTVWLTENAWVQHPHQASTIGMLRSIRREHPDLGVHVLDVDAVETFDATFLVEQVLRLEEHTDELASSTTWTQEPEVSWCKGRPWIPRLKRDLARNNRMNSSRRPIYEMIDSSRAPVALQTARDSSSYFLESAETWFVPESVQQMETKTIYVHFSCPHALRVGQLGFFYLVQGHVQEGNREVPVVALAERNASIVHVRPDYIYTEADNNLSEGGGSLMVTVLAAAVLAETVISTAKCLGVTDSILVLNPPSICGQMLLHAGEEIGLQVHLATTSGNRSSVSAGDAKSWLTLHARDTDWHLRRVLPRGVQALVDLSADQSCEGLTQRMMKVLMPGCAHYRAADLFTDTVSTELHSGSRHQASLPAAYWEHVVSLARQGLPSVSEGWEVMPCTQFAAHADKTRPDLSTVISWPRESDEATLPTRVRSIDAETLFAADKTYLLVGLTGDLGRSLGRWMVQHGACHIVLTSRNPQVNPKWLAHVEELGGRVTVLSMDVTSQNSVEAGLAKLKDLHLPPVGGIAFGPLVLQDVMLNNMELPMMEMVLNPKVEGVRILHEKFSDPTSSNPLDFFVMFSSIVAVMGNPGQANYSAANCYLQALAQQRVASGLAASTIDIGAVYGVGFVTRAELEEDFNAIRFMFDSVEEHELHTLFAEAVVAGRRAVHQQEQQRKFATVLDMADLELTTGIPPLDPALKDRITFFDDPRIGNLKIPEYRGAKAGEGAAGSKGSVKEQLLQATNLDQVRQIVIDGLSAKLQVTLQIPDGESVHPTIPLIDQGVDSLGAVTVGTWFSKQLYLDLPLLKVLGGASITDLANEAAARLPPSSIPLVAATDGGAESTDNTSENEVSGREDTDLSAAATITEPSSADEDDTEPGDEDVPRSHHPLSLGQEYSWRIQQGAEDPTVFNNTIGMFMKGSIDLKRLYKALRAVLRRHEIFRTGFANVDENGMAQLVFGQTKNKVQTIQVSDRAGAEEGYRQLVQTRYNPAAGDTLRLVDFFWGQDDHLLVVAYHRLVGDGSTTENIFVEAGQLYDGTSLSPHVPQFADLAARQRAMLEDGRMEEDLAYWKKMHYRPSSIPVLPLMRPLVGNSSRSDTPNFQHCGPWQQHEAVARLDPMVAFRIKERSRKHKATPMQFYLAAYQVLLARLTDSTDLTVGLADTNRATVDEMAAMGFFANLLPLRFRDFRPHITFGEHLIATRDLVREALQHARVPYGVLLDQLGLEVPVPTSNQPAPLFQAVFDYKQGQAESGTIGGAKITEVIATRERTPYDVVLEMSDDPTKDPLLTAKLQSSRYEAHHPQAFLESYMSLLSMFSMNPALKLA lovb-lovc-1 3241779 N/A 3241780 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3241781 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false