DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2012-20 in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251057X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation lee-2012-21 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251053X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation lee-2012-22 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251055X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation lee-2012-23 in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251060X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation lee-2012-24 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251058X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation lee-2012-25 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251054X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation lee-2012-26 ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251056X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation lee-2012-27 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight by comassie molecular weight estimation by coomasie staining weight by comassie Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. DIP original experiment: DIP-251059X Nature structural & molecular biology 2012 IMEx Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Lee CH., Kim MS., Chung BM., Leahy DJ., Coulombe PA. Only protein-protein interactions imex curation k1c14_human Keratin, type I cytoskeletal 14 Cytokeratin-14 KRT14 Keratin-14 protein protein human Homo sapiens Human MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGACGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN k2c5_human Keratin, type II cytoskeletal 5 Cytokeratin-5 58 kDa cytokeratin KRT5 Keratin-5 Type-II keratin Kb5 protein protein human Homo sapiens Human MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS krt14-krt5-2 3241323 N/A 3241331 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3241332 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false krt14-krt5-3 3241324 N/A 3241331 unspecified role unspecified role bait bait Region 332-421 Region 332-421 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3241332 unspecified role unspecified role prey prey Region 382-476 Region 382-476 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false krt14-krt5-4 3241325 N/A 3241331 unspecified role unspecified role unspecified role unspecified role Region 332-421 Region 332-421 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3241332 unspecified role unspecified role unspecified role unspecified role Region 382-476 Region 382-476 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false krt14-1 3241326 N/A 3241331 unspecified role unspecified role unspecified role unspecified role Region 332-472 Region 332-472 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false krt14-krt5-5 3241327 N/A 3241331 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3241332 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false krt14-krt5-6 3241328 N/A 3241331 unspecified role unspecified role unspecified role unspecified role Region 332-421 Region 332-421 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3241332 unspecified role unspecified role unspecified role unspecified role Region 382-476 Region 382-476 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false krt14-krt5-7 3241329 N/A 3241331 unspecified role unspecified role unspecified role unspecified role Region 332-421 Region 332-421 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3241332 unspecified role unspecified role unspecified role unspecified role Region 382-476 Region 382-476 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli disulfide bond disulfide bond disulfide bridge SS-bond false false false krt14-krt5-8 3241330 N/A 3241331 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3241332 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false