DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhang-2012-76 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250449X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-77 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250442X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-78 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250447X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-79 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250445X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-80 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250451X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-81 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250443X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-82 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250450X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-83 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250448X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-84 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250446X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation zhang-2012-85 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. DIP original experiment: DIP-250444X Structure (London, England : 1993) 2012 IMEx Structural basis for calmodulin as a dynamic calcium sensor. Zhang M., Abrams C., Wang L., Gizzi A., He L., Lin R., Chen Y., Loll PJ., Pascal JM., Zhang JF. Only protein-protein interactions imex curation h6vq94_rat Kcnn2 protein protein rat Rattus norvegicus (Rat) Rat MSSCRYNGGVMRPLSNLSSSRRNLHEMDSEAQPLQPPASVVGGGGGASSPSAAAAASSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQARKLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIETQMENYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK kcnn2_rat Small conductance calcium-activated potassium channel protein 2 Kcnn2 KCa2.2 protein protein rat Rattus norvegicus (Rat) Rat MSSCRYNGGVMRPLSNLSSSRRNLHEMDSEAQPLQPPASVVGGGGGASSPSAAAAASSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIETQMENYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS calm1-kcnn2-3 3240425 N/A 3240435 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 412-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role AEDANS-label AEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-4 3240426 N/A 3240435 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 412-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-5 3240427 N/A 3240437 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 413-489 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-6 3240428 N/A 3240435 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 412-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-7 3240429 N/A 3240435 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 412-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role AEDANS-label AEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-8 3240430 N/A 3240435 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 412-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-9 3240431 N/A 3240437 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 413-489 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role AEDANS-label AEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-10 3240432 N/A 3240437 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 413-489 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role AEDANS-label AEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-11 3240433 N/A 3240437 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 413-489 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false calm1-kcnn2-12 3240434 N/A 3240435 unspecified role unspecified role unspecified role unspecified role Calmodulin binding domain Region 412-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3240436 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false