DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ guo-2012-5 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. DIP original experiment: DIP-251649X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase. Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. Only protein-protein interactions imex curation guo-2012-6 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. DIP original experiment: DIP-251652X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase. Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. Only protein-protein interactions imex curation guo-2012-7 ecolx Escherichia coli reverse phase chrom reverse phase chromatography reverse phase chrom predetermined predetermined participant predetermined Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. DIP original experiment: DIP-251650X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase. Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. Only protein-protein interactions imex curation guo-2012-8 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. DIP original experiment: DIP-251653X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase. Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. Only protein-protein interactions imex curation guo-2012-9 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. DIP original experiment: DIP-251651X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase. Guo W., West JM., Dutton AS., Tsuruta H., Kantrowitz ER. Only protein-protein interactions imex curation pyri_ecoli Aspartate carbamoyltransferase regulatory chain pyrI b4244 JW4203 protein protein ecoli Escherichia coli (strain K12) MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITIGLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPERIDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN pyrb_ecoli Aspartate carbamoyltransferase catalytic subunit pyrB b4245 Aspartate transcarbamylase JW4204 protein protein ecoli Escherichia coli (strain K12) MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL pyrb-pyri-4 3239908 N/A 3239913 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3239914 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pyrb-pyri-14 3239909 N/A 3239913 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3239914 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pyrb-pyri-15 3239910 N/A 3239913 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3239914 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pyrb-pyri-16 3239911 N/A 3239913 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3239914 unspecified role unspecified role unspecified role unspecified role P0A786:p.[Lys165Glu;Glu240Lys] K165E/E240K mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pyrb-pyri-17 3239912 N/A 3239913 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3239914 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false