DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ohto-2012-1 drome Drosophila melanogaster (Fruit fly) Fruit fly x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251646X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions imex curation ohto-2012-2 drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251644X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions imex curation ohto-2012-3 drome Drosophila melanogaster (Fruit fly) Fruit fly saxs x ray scattering saxs predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251648X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions imex curation ohto-2012-4 drome Drosophila melanogaster (Fruit fly) Fruit fly saxs x ray scattering saxs predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251647X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions imex curation ohto-2012-5 drome Drosophila melanogaster (Fruit fly) Fruit fly comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251645X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions imex curation ly96_mouse Lymphocyte antigen 96 Protein MD-2 ESOP-1 Ly96 Esop1 Md2 protein protein mouse Mus musculus Mouse MLPFILFSTLLSPILTESEKQQWFCNSSDAIISYSYCDHLKFPISISSEPCIRLRGTNGFVHVEFIPRGNLKYLYFNLFISVNSIELPKRKEVLCHGHDDDYSFCRALKGETVNTSIPFSFEGILFPKGHYRCVAEAIAGDTEEKLFCLNFTIIHRRDVN tlr4_mouse Toll-like receptor 4 Tlr4 Lps protein protein mouse Mus musculus Mouse MMPPWLLARTLIMALFFSCLTPGSLNPCIEVVPNITYQCMDQKLSKVPDDIPSSTKNIDLSFNPLKILKSYSFSNFSELQWLDLSRCEIETIEDKAWHGLHHLSNLILTGNPIQSFSPGSFSGLTSLENLVAVETKLASLESFPIGQLITLKKLNVAHNFIHSCKLPAYFSNLTNLVHVDLSYNYIQTITVNDLQFLRENPQVNLSLDMSLNPIDFIQDQAFQGIKLHELTLRGNFNSSNIMKTCLQNLAGLHVHRLILGEFKDERNLEIFEPSIMEGLCDVTIDEFRLTYTNDFSDDIVKFHCLANVSAMSLAGVSIKYLEDVPKHFKWQSLSIIRCQLKQFPTLDLPFLKSLTLTMNKGSISFKKVALPSLSYLDLSRNALSFSGCCSYSDLGTNSLRHLDLSFNGAIIMSANFMGLEELQHLDFQHSTLKRVTEFSAFLSLEKLLYLDISYTNTKIDFDGIFLGLTSLNTLKMAGNSFKDNTLSNVFANTTNLTFLDLSKCQLEQISWGVFDTLHRLQLLNMSHNNLLFLDSSHYNQLYSLSTLDCSFNRIETSKGILQHFPKSLAFFNLTNNSVACICEHQKFLQWVKEQKQFLVNVEQMTCATPVEMNTSLVLDFNNSTCYMYKTIISVSVVSVIVVSTVAFLIYHFYFHLILIAGCKKYSRGESIYDAFVIYSSQNEDWVRNELVKNLEEGVPRFHLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSRHFIQSRWCIFEYEIAQTWQFLSSRSGIIFIVLEKVEKSLLRQQVELYRLLSRNTYLEWEDNPLGRHIFWRRLKNALLDGKASNPEQTAEEEQETATWT ly96_human Lymphocyte antigen 96 Protein MD-2 ESOP-1 LY96 ESOP1 MD2 protein protein human Homo sapiens Human MLPFLFFSTLFSSIFTEAQKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKRSKGLLHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFSFKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN o00206-1 Toll-like receptor 4 TLR4 hToll protein protein human Homo sapiens Human MMSASRLAGTLIPAMAFLSCVRPESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI ly96-tlr4-12 3239840 N/A 3239845 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3239846 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false ly96-tlr4-13 3239841 N/A 3239845 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3239846 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false ly96-tlr4-14 3239842 N/A 3239847 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3239848 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 23-629 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false ly96-tlr4-15 3239843 N/A 3239845 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3239846 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false ly96-tlr4-16 3239844 N/A 3239845 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3239846 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false