DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ fodor-2011-1 ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. DIP original experiment: DIP-249390X PLoS biology 2011 IMEx Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. Only protein-protein interactions imex curation fodor-2011-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. DIP original experiment: DIP-249385X PLoS biology 2011 IMEx Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. Only protein-protein interactions imex curation fodor-2011-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. DIP original experiment: DIP-249388X PLoS biology 2011 IMEx Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. Only protein-protein interactions imex curation fodor-2011-4 in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. DIP original experiment: DIP-249386X PLoS biology 2011 IMEx Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. Only protein-protein interactions imex curation fodor-2011-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. DIP original experiment: DIP-249387X PLoS biology 2011 IMEx Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. Only protein-protein interactions imex curation fodor-2011-6 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. DIP original experiment: DIP-249389X PLoS biology 2011 IMEx Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Fodor K., Wolf J., Erdmann R., Schliebs W., Wilmanns M. Only protein-protein interactions imex curation spya_human Serine--pyruvate aminotransferase Alanine--glyoxylate aminotransferase AGXT AGT1 SPAT protein protein human Homo sapiens Human MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL p50542-1 Peroxisomal targeting signal 1 receptor PEX5 PXR1 PTS1-BP Peroxin-5 Peroxisomal C-terminal targeting signal import receptor Peroxisome receptor 1 protein protein human Homo sapiens Human MAMRELVEAECGGANPLMKLAGHFTQDKALRQEGLRPGPWPPGAPASEAASKPLGVASEDELVAEFLQDQNAPLVSRAPQTFKMDDLLAEMQQIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTATDRWYDEYHPEEDLQHTASDFVAKVDDPKLANSEFLKFVRQIGEGQVSLESGAGSGRAQAEQWAAEFIQQQGTSDAWVDQFTRPVNTSALDMEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSATYDKGYQFEEENPLRDHPQPFEEGLRRLQEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNQTALMALAVSFTNESLQRQACETLRDWLRYTPAYAHLVTPAEEGAGGAGLGPSKRILGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLSTLLTMFGLPQ agxt-1 3239802 N/A 3239808 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false agxt-pex5-1 3239803 N/A 3239809 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 315-639 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3239808 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false agxt-pex5-2 3239804 N/A 3239809 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 315-639 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3239808 unspecified role unspecified role unspecified role unspecified role P21549:p.Tyr330Ala Y330A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false agxt-pex5-3 3239805 N/A 3239809 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 315-639 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3239808 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false agxt-pex5-4 3239806 N/A 3239809 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 315-639 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3239808 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false agxt-2 3239807 N/A 3239808 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false