DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yu-2012-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yu AC., Worrall LJ., Strynadka NC. DIP original experiment: DIP-249323X Structure (London, England : 1993) 2012 IMEx Structural insight into the bacterial mucinase StcE essential to adhesion and immune evasion during enterohemorrhagic E. coli infection. Yu AC., Worrall LJ., Strynadka NC. Only protein-protein interactions imex curation yu-2012-7 in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Yu AC., Worrall LJ., Strynadka NC. DIP original experiment: DIP-249324X Structure (London, England : 1993) 2012 IMEx Structural insight into the bacterial mucinase StcE essential to adhesion and immune evasion during enterohemorrhagic E. coli infection. Yu AC., Worrall LJ., Strynadka NC. Only protein-protein interactions imex curation yu-2012-8 in vitro In vitro protease assay protease assay weight by staining molecular weight estimation by staining weight by staining Yu AC., Worrall LJ., Strynadka NC. DIP original experiment: DIP-249325X Structure (London, England : 1993) 2012 IMEx Structural insight into the bacterial mucinase StcE essential to adhesion and immune evasion during enterohemorrhagic E. coli infection. Yu AC., Worrall LJ., Strynadka NC. Only protein-protein interactions imex curation ic1_human Plasma protease C1 inhibitor SERPING1 C1IN C1NH C1 esterase inhibitor C1-inhibiting factor Serpin G1 protein protein human Homo sapiens Human MASRLTLLTLLLLLLAGDRASSNPNATSSSSQDPESLQDRGEGKVATTVISKMLFVEPILEVSSLPTTNSTTNSATKITANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGSFCPGPVTLCSDLESHSTEAVLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPRA stce_eco57 Metalloprotease StcE stcE tagA L7031 ECO57PM83 Mucinase Neutral zinc metalloprotease StcE Secreted protease of C1 esterase inhibitor from EHEC protein protein eco57 Escherichia coli O157:H7 MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYFNTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTPVQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVNKLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTVFYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLVIKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSRMIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLNSTAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGHNYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPFDGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADTQEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASADNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPEQFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQLRFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDNLSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK serping1-stce-1 3239103 N/A 3239106 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3239107 unspecified role unspecified role unspecified role unspecified role Mature protein Region 36-898 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false serping1-stce-2 3239104 N/A 3239106 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3239107 unspecified role unspecified role unspecified role unspecified role Mature protein Region 36-898 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false serping1-stce-3 3239105 N/A 3239106 enzyme target enzyme target substrate substrate unspecified role unspecified role human Homo sapiens Human N/A 3239107 enzyme enzyme unspecified role unspecified role Mature protein Region 36-898 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli protein cleavage protein cleavage false false false Deletion of region 140-243 decreased the interaction rate/strength.