DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2012-12 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang X., Loram LC., Ramos K., de Jesus AJ., Thomas J., Cheng K., Reddy A., Somogyi AA., Hutchinson MR., Watkins LR., Yin H. DIP original experiment: DIP-251608X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Morphine activates neuroinflammation in a manner parallel to endotoxin. Wang X., Loram LC., Ramos K., de Jesus AJ., Thomas J., Cheng K., Reddy A., Somogyi AA., Hutchinson MR., Watkins LR., Yin H. Only protein-protein interactions imex curation wang-2012-13 in vitro In vitro solid phase assay solid phase assay anti tag western anti tag western blot anti tag western Wang X., Loram LC., Ramos K., de Jesus AJ., Thomas J., Cheng K., Reddy A., Somogyi AA., Hutchinson MR., Watkins LR., Yin H. DIP original experiment: DIP-251609X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Morphine activates neuroinflammation in a manner parallel to endotoxin. Wang X., Loram LC., Ramos K., de Jesus AJ., Thomas J., Cheng K., Reddy A., Somogyi AA., Hutchinson MR., Watkins LR., Yin H. Only protein-protein interactions imex curation o00206-1 Toll-like receptor 4 TLR4 hToll protein protein human Homo sapiens Human MMSASRLAGTLIPAMAFLSCVRPESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI ly96_human Lymphocyte antigen 96 Protein MD-2 ESOP-1 LY96 ESOP1 MD2 protein protein human Homo sapiens Human MLPFLFFSTLFSSIFTEAQKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKRSKGLLHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFSFKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN tlr4-tlr4-1 3238654 N/A 3238656 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse Mus musculus Mouse N/A 3238656 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse Mus musculus Mouse physical association physical association false false false ly96-tlr4-11 3238655 N/A 3238656 unspecified role unspecified role prey prey Fc-tag Fc-tag fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3238657 unspecified role unspecified role bait bait Region 95-111 Region 95-111 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false