DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gudesblat-2012-1 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gudesblat GE., Schneider-Pizoń J., Betti C., Mayerhofer J., Vanhoutte I., van Dongen W., Boeren S., Zhiponova M., de Vries S., Jonak C., Russinova E. DIP original experiment: DIP-268061X Nature cell biology 2012 IMEx SPEECHLESS integrates brassinosteroid and stomata signalling pathways. Gudesblat GE., Schneider-Pizoń J., Betti C., Mayerhofer J., Vanhoutte I., van Dongen W., Boeren S., Zhiponova M., de Vries S., Jonak C., Russinova E. Only protein-protein interactions imex curation gudesblat-2012-2 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gudesblat GE., Schneider-Pizoń J., Betti C., Mayerhofer J., Vanhoutte I., van Dongen W., Boeren S., Zhiponova M., de Vries S., Jonak C., Russinova E. DIP original experiment: DIP-268062X Nature cell biology 2012 IMEx SPEECHLESS integrates brassinosteroid and stomata signalling pathways. Gudesblat GE., Schneider-Pizoń J., Betti C., Mayerhofer J., Vanhoutte I., van Dongen W., Boeren S., Zhiponova M., de Vries S., Jonak C., Russinova E. Only protein-protein interactions imex curation ksg7_arath Shaggy-related protein kinase eta ASK7 BIN2 DWF12 UCU1 ASK-eta Protein BRASSINOSTEROID INSENSITIVE 2 Protein ULTRACURVATA 1 F28A21.120 At4g18710 SK21 Shaggy-related protein kinase 21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADDKEMPAAVVDGHDQVTGHIISTTIGGKNGEPKQTISYMAERVVGTGSFGIVFQAKCLETGETVAIKKVLQDRRYKNRELQLMRVMDHPNVVCLKHCFFSTTSKDELFLNLVMEYVPESLYRVLKHYSSANQRMPLVYVKLYMYQIFRGLAYIHNVAGVCHRDLKPQNLLVDPLTHQVKICDFGSAKQLVKGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKVLGTPTREEIRCMNPHYTDFRFPQIKAHPWHKIFHKRMPPEAIDFASRLLQYSPSLRCTALEACAHPFFDELREPNARLPNGRPFPPLFNFKQEVAGSSPELVNKLIPDHIKRQLGLSFLNQSGT spch_arath Transcription factor SPEECHLESS SPCH BHLH98 EN19 Basic helix-loop-helix protein 98 Transcription factor EN 19 bHLH transcription factor bHLH098 MFH8.15 At5g53210 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQEIIPDFLEECEFVDTSLAGDDLFAILESLEGAGEISPTAASTPKDGTTSSKELVKDQDYENSSPKRKKQRLETRKEEDEEEEDGDGEAEEDNKQDGQQKMSHVTVERNRRKQMNEHLTVLRSLMPCFYVKRGDQASIIGGVVEYISELQQVLQSLEAKKQRKTYAEVLSPRVVPSPRPSPPVLSPRKPPLSPRINHHQIHHHLLLPPISPRTPQPTSPYRAIPPQLPLIPQPPLRSYSSLASCSSLGDPPPYSPASSSSSPSVSSNHESSVINELVANSKSALADVEVKFSGANVLLKTVSHKIPGQVMKIIAALEDLALEILQVNINTVDETMLNSFTIKIGIECQLSAEELAQQIQQTFC ask7-3 3238368 N/A 3238370 putative self putative self unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false ask7-spch-1 3238369 N/A 3238371 enzyme target enzyme target substrate substrate unspecified role unspecified role pS/T pS/T phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q700C7:p.Ser65Ala S65A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q700C7:p.[Ser38Ala;Thr44Ala] S38A/T44A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q700C7:p.[Ser38Ala;Thr44Ala;Ser65Ala] S38A/T44A/S65A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3238370 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false