DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yang-2012-5 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yang X., Jin H., Cai X., Li S., Shen Y. DIP original experiment: DIP-251582X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural and mechanistic insights into the activation of Stromal interaction molecule 1 (STIM1). Yang X., Jin H., Cai X., Li S., Shen Y. Only protein-protein interactions imex curation yang-2012-6 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yang X., Jin H., Cai X., Li S., Shen Y. DIP original experiment: DIP-251585X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural and mechanistic insights into the activation of Stromal interaction molecule 1 (STIM1). Yang X., Jin H., Cai X., Li S., Shen Y. Only protein-protein interactions imex curation yang-2012-7 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Yang X., Jin H., Cai X., Li S., Shen Y. DIP original experiment: DIP-251586X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural and mechanistic insights into the activation of Stromal interaction molecule 1 (STIM1). Yang X., Jin H., Cai X., Li S., Shen Y. Only protein-protein interactions imex curation yang-2012-8 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Yang X., Jin H., Cai X., Li S., Shen Y. DIP original experiment: DIP-251583X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural and mechanistic insights into the activation of Stromal interaction molecule 1 (STIM1). Yang X., Jin H., Cai X., Li S., Shen Y. Only protein-protein interactions imex curation yang-2012-9 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Yang X., Jin H., Cai X., Li S., Shen Y. DIP original experiment: DIP-251584X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural and mechanistic insights into the activation of Stromal interaction molecule 1 (STIM1). Yang X., Jin H., Cai X., Li S., Shen Y. Only protein-protein interactions imex curation stim1_human Stromal interaction molecule 1 STIM1 GOK protein protein human Homo sapiens Human MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK stim1_caeel Stromal interaction molecule 1 stim-1 Y55B1BM.1 protein protein caeel Caenorhabditis elegans MGRVSWIIALYLTINVVIVVNGDRVTRNVEVTAEEEKIRDKLGYEAIRDIHRDMDDDHSGSIDRNESTGFMKEDMQMRGSERTRRENKFHGDDDAITVDDLWEAWFESIERTWTNERLVEWLINDVNLPSIVEAVKAKKIDGKILPRFASPNSDFLNKELGIKSSVYRQKLRLNSLDVVLFGYKDNNNRTKDILLAFLALLLTSLIFLYVRQKQKAQQKVNELSNKLTELKCMETEFEDVQKMLNDERSKRSISDGVVNHTEMENLRVQLEEAERRLEANSNGSQAPLALQPLLRRTCENEMAFLEKQRQDCFKEMKEAIEMVDRLQKKQGSVLSSLKLATGAASTSDQVDSKIFALKSRMEKIHTLTRETQERWLQIESLCGFPLLYLNETEHINRSIASSHFYNKSHEGSSSSGSISNAHSNPNAVNSNFVKKVSPPIPPSQQTANLRFVPTEQSDSIHSEDTSPIVEDVAISRSLTQDLAEADMQSIVSGSTNGSGSVAALKKRKGIFPKLFRRNTSKSSSLGGTSN crcm1_human Calcium release-activated calcium channel protein 1 ORAI1 CRACM1 TMEM142A Protein orai-1 Transmembrane protein 142A protein protein human Homo sapiens Human MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA stim1-4 3238256 N/A 3238261 unspecified role unspecified role unspecified role unspecified role Region 344-444 Region 344-444 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false stim_1-1 3238257 N/A 3238262 unspecified role unspecified role unspecified role unspecified role Region 256-391 Region 256-391 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false orai1-stim1-15 3238258 N/A 3238263 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3238261 unspecified role unspecified role prey prey Region 344-444 Region 344-444 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false EGFP-tag EGFP-tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13586:p.[Leu347Ala;Trp350Ala;Leu351Ala] L347A/W350A/L351A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13586:p.[Trp430Ala;Ile433Ala;Leu436Ala] W430A/I433A/L436A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13586:p.[Lys384Ala;Lys385Ala;Lys386Ala] K384A/K385A/K386A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13586:p.[Lys382Ala;Lys384Ala;Lys385Ala;Lys386Ala;Arg387Ala] K382A/K384A/K385A/K386A/R387A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false stim1-7 3238259 N/A 3238261 unspecified role unspecified role unspecified role unspecified role Region 344-444 Region 344-444 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false stim_1-2 3238260 N/A 3238262 unspecified role unspecified role unspecified role unspecified role Region 256-391 Region 256-391 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false