DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wiener-2012-1 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249451X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249453X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-3 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249460X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-4 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249455X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249454X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-6 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249456X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-7 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249458X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-8 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249452X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-9 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249457X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation wiener-2012-10 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249459X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions imex curation ube2n_human Ubiquitin-conjugating enzyme E2 N Ubiquitin-protein ligase N Ubiquitin carrier protein N Ubc13 Bendless-like ubiquitin-conjugating enzyme UBE2N BLU UbcH13 E2 ubiquitin-conjugating enzyme N protein protein human Homo sapiens Human MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI q96fw1-1 Ubiquitin thioesterase OTUB1 OTUB1 OTB1 OTU1 Deubiquitinating enzyme OTUB1 OTU domain-containing ubiquitin aldehyde-binding protein 1 Otubain-1 Ubiquitin-specific-processing protease OTUB1 HSPC263 Otubain-1 protein protein human Homo sapiens Human MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK q13404-2 Ubiquitin-conjugating enzyme E2 variant 1 UBE2V1 CROC1 UBE2V UEV1 P/OKcl.19 CROC-1A UEV-1A CROC-1 TRAF6-regulated IKK activator 1 beta Uev1A protein protein human Homo sapiens Human MPGEVQASYLKSQSKLSDEGRLEPRKFHCKGSKSPSQFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG otubl_caeel Ubiquitin thioesterase otubain-like C25D7.8 Deubiquitinating enzyme otubain-like otub-1 Ubiquitin-specific-processing protease otubain-like protein protein caeel Caenorhabditis elegans MANEPQKSDDNGQAAEAVVTDDEIVLQDQQLKTIEDEQKSVPLVATLAPFSILCAEYDNETSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKFIASSRDWTRTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHTEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGWHYDIPSDDQQIAPEITLLYRPGHYDVIYKKDSTEASEIEN otub1-ube2n-2 3237365 N/A 3237375 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3237376 unspecified role unspecified role unspecified role unspecified role Q96FW1-1:p.[Phe138Ala;Tyr170Ala] Y170A/F138A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ube2n-ube2v1-5 3237366 N/A 3237375 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3237377 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false otub1-ube2n-3 3237367 N/A 3237377 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237375 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237376 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli association association false false false ub-10 3237368 N/A 3237378 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false otub1-ube2n-4 3237369 N/A 3237376 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3237375 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3237377 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false otub1-ube2n-5 3237370 N/A 3237375 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237376 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false otub_1-ube2n-1 3237371 N/A 3237379 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3237375 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false otub_1-ube2n-2 3237372 N/A 3237379 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3237375 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false otub1-ub-1 3237373 N/A 3237378 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237375 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237376 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli association association false false false otub1-ub-2 3237374 N/A 3237377 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237378 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237375 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3237376 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli association association false false false