DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhang-2012-49 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. DIP original experiment: DIP-251547X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. Only protein-protein interactions imex curation zhang-2012-50 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. DIP original experiment: DIP-251548X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. Only protein-protein interactions imex curation c6ktb8_plaf7 PF3D7_0628200 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MCNFIKKGIRFNGDKYIFLFDIFIKKYLLNVFVANILWEEENNICFLNRLKNKRKKSILFLKEEYLRYLMILNKEEKNKKKRLKKIYECIKVFSIKEFRWLINKLEIIYFYFFCHLLLLCIFQNIFLLTYMSKEYFLKNIHDYMINILSNDIKFNTCIEFTHNDKYEQKCITMAYYDFLLNKKGKLKKRNKYYDIKNIEPLTGKDKNLYSYYNFSPFFPMSSSDIINVNTNDKISNILWNDKYIDTLNHVNMKKKDIPLNIHSNNILMNNYAYRKYVRSYMIKKRINDLILYNLKNIFEKINNIEKLTNINNFMNFKKEYEKVSKEVCNNNNNNYDPSDYMLILPNSSKDHALYNNINNDEYMYKKFCNFISQAREIKKQREKEKCHRDEKCDRGENYDRGEKYDRDEKYDKEEKGLKEDNYRDVNEWNSNKSERCNKIYPSDYKFFLSEDKKYDTPYYSNERVDFHNSDIYMNDMDEELYYSSYHNNHHNNDITYNSNVYKRMLNEVIHPSVESNDVKKGPLNNDNIKNKESNVYEINDIIYQLNGEKKMNTNMCEKRGNKIFDSNNFHNINERKKKILDENDMITIDNNIDKKENILFPYFHMEILKDNEMNITKYYKDKQYYGQYKYDENIYIYDLIVLDTSGYIYKVSTDGSYHWKRKIVDHIQDYSNIEEKDDERKVLKKRNKLNEKDMYESNNKYESNMYDMNTYESNKYDINTYDKNISDSTKAHQNIYVLKKNVYDMNYDSKRALKISPYHNYFNTSIINSINRDKLKHSKPLYKNNIKTNDDNKKLRENKTKNKRLVSNYLGNLFYINENNEVIPLNINIKDVVNNSPFKSPLFPNMVFIGSRESTIINLDYDTGHVLRKYDEASNELLDKPPKKKSRKNKSIKNVKDINDNMKLYKEGSQNVEELSFTEEENKKKSILEGEKIEVASLNNEENMNKGFVCEKDDKINNYDNDEDDLFYEQYEDNNDNDNNKNDNNKNDNNKNDNNKNDNNNNNNNNNNNSYYYYNINGNASDDILVHSKNELLNNTYINKTNIKDENSNNNESFLNKIDGMNNFKLLPKRNMKRNRKKNLLKRLMVSTNKLSMLLNRYHLINRSLERMRKDIKRGKNKRILKKRQLQISLIKWVIKAVDENTLKKKWITTWVDVGSIFMTDVHRKDTSFINSLIEIVGNKLILRPIEKDKMKSTTYQILKIMNNDTDEIQMYRNEDMKYEVINNVDDINNVDNINNANNMNNVNNINNMNNMNNMNNMNNMNNMNNINNMNNINNMNNINNINNINNMNNILNDRLKNRINNKDNSNIKSKIFIFSESISSVFAVKYKNLSNIFTLDIIAKPNIKLYSDYDNLNNFTYNPVHVKKERTLFLPFSKDISDLDGDKFSCSFEDNIIYGKRLIHRLNSISVNISSIEKDMKYLLSNIIYVYDKNKRIPISYIYDMKNLIYEYQKVKQEFLYHLPWDEGDQKYLSRTDDVLNNSIIDNIGKKGPIFICEYINKFMDLYFEENDICYDYCSMLNIWDKIFNNTVSDGDSLLLSNLYRVVHNAFKNNNKYNNNNIYFDNNINININSSSSSSSRSRRNIYNFDNYYNNRRDYNSFWEDRHNILMNRENFLINTSTDKVFSGGKNNELKEFTSIRYKRRRWYWRVFYTIMFIIFFPVLFIYRRIIKRRKGSSKGNKIGTSSNNKLIKKNRTFKDYEDDENNIMSEDEEDDLDMNYDLIFDDDRLKVKKIKRMRKNYNNNNNNNNNKNNNNISNNNSNSNSKSNRFLSKLSNIDLANIDLNLIKKSHGKKMDNFEQPTLVDILARHARDSTHNDGVSYYPFNENETYNMLSLNYAWGGNHKHMNVERTSEYNMGNISHQLNYNNIRNLGDNKISAYELDIYEKELFHLYRRRAASQDVLNKKSFVMKKRIRSSYKVGSSNKYHKKNYTDNEKDKKKYRSYKEKHINEKMFDKKEFLNFLTNFNKKFMKKNSLVDHLIKMNDKAEDNYDGYNSSGSRYNNINDDGVELCGTKRYTNNKNNSDYDNYNNNNNMKNKRYSNKKHNNDNIIINNNNNKYTDERKYRNKSIKEDVDYTNDYYNIQLNNNKINNNQTKNKIDTIRNISHEKLGNNKSSSARNLSLIQTSHIPYDAPLADFLENGRFLRTFENISLIGQGGFGSVYKVSHRLEPGSPTYAVKFIYLKVSSLDNVSSRRYFREIAANRDIYSKHVVRYYTWWCEEPQFLPMHLMPKEIQNLVKKNKDTFKKRLTKNKKYSNNCISDSSNNNNSSCYSASSYNSSINSNYRNMKLWIKKKEQSPDMKRYKEVLRKNNAPNLVFYSDNDGLTSKNKENPEKNHNPFLSDKNFSDSIYKKKKSHDYNSSSHKLKKRKNKKKKSKKKRKSKSKIKTNAQGIYEESENDEGRDHFQYKKGKEQFSKFIGKHNSMGFTQSFQEYDPFDNGYLSEEDRDLIVFADNEESNGNDQQMIRHDNMNNENVIIKHRNEDDKNGLDGDKNGLDGDKNGLDGDKNGLDGDKNGLDGDKNELDGDKNGLDGDKNGLDGDKNGLDGDKNELDDNTKKLDDNTKKLDDLLMKQKINSLTRNDIVNIENENPAPHATNNIKNKKVDLNGELTYYDYVGKNEVIPNSRTETNVESINTNGMFNNKFSVMKDEGGEYKKKENMTWGDTKRDGLYENGKHEKDGLGVNKCITNKYIENDDDDDDDDDNNNNNNNIDERKKDLKKKQKNAITKGNEDLLATNGTNNKEKRKKDDDINKNMEKIKSYKKKTPVPEFSIVLLLQMELCKGYTLRKWLDRSTRSDKPLHFTYSDKKMNHPLEFDLFKQLIKGLKDIHATCFIHRDLKPENIFVDPDTYTLKIGDLGLVRFIEEKKREKDFNNIDCYKDNIYTDINQNAITSQISIKGQIIGTPGYTAPEGGALCDEKADIYSAALILLELLCPRFTTIMERYKRLNDFRNYYTVPDYVKIHLNPWYILMLQMSKPNPADRPSAADVYSKIKVLLDPHLTDFAFSFNDIHNEHMNKPPQGTNNFERITDNKDKFVIQSVVDMKNKVENEEIPIEKGLNSNVENIKNENNGADK q8ibh7_plaf7 PF3D7_0728000 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MTEMRVKADLGDCRFYKKKFPEVDDLIMVKVNRIEDMGAYVSILEYNDMEGMILMSELSKRRFRSVNKLIRVGRHEVVLVLRVDSQKGYIDLSKRRVSPKDIIKCEEKFSKSKKVHQTVRHVAKEHGITVEELNEKAIWPLYERYGHALDALKEATMNPENVFKGLDISEEIKNSLLKDIKLRLTPQALKLRGRIDVWCFGYEGIDAVKEALKKGKEISNDKVSINIKLIAPPQYVIVTSCHDKDLGMAKIQEAMKVISDKIKEYKGGDFKQQGEILVIGGDEEKRLEELLDKHDGISSDDDDYNTSDEDDENSSEEDENTSEDEEEED c6ktb8_plaf7-1 3237296 N/A 3237298 putative self putative self unspecified role unspecified role Region 2109-2236/2758-3072 Region 2109-2236/2758-3072 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false c6ktb8_plaf7-q8ibh7_plaf7-1 3237297 N/A 3237299 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3237298 enzyme enzyme unspecified role unspecified role Region 2109-2236/2758-3072 Region 2109-2236/2758-3072 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false