DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ daboussi-2012-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Daboussi L., Costaguta G., Payne GS. DIP original experiment: DIP-268053X Nature cell biology 2012 IMEx Phosphoinositide-mediated clathrin adaptor progression at the trans-Golgi network. Daboussi L., Costaguta G., Payne GS. Only protein-protein interactions imex curation daboussi-2012-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Daboussi L., Costaguta G., Payne GS. DIP original experiment: DIP-268055X Nature cell biology 2012 IMEx Phosphoinositide-mediated clathrin adaptor progression at the trans-Golgi network. Daboussi L., Costaguta G., Payne GS. Only protein-protein interactions imex curation daboussi-2012-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Daboussi L., Costaguta G., Payne GS. DIP original experiment: DIP-268054X Nature cell biology 2012 IMEx Phosphoinositide-mediated clathrin adaptor progression at the trans-Golgi network. Daboussi L., Costaguta G., Payne GS. Only protein-protein interactions imex curation ncs1_yeast Calcium-binding protein NCS-1 FRQ1 NCS1 YDR373W D9481.6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGAKTSKLSKDDLTCLKQSTYFDRREIQQWHKGFLRDCPSGQLAREDFVKIYKQFFPFGSPEDFANHLFTVFDKDNNGFIHFEEFITVLSTTSRGTLEEKLSWAFELYDLNHDGYITFDEMLTIVASVYKMMGSMVTLNEDEATPEMRVKKIFKLMDKNEDGYITLDEFREGSKVDPSIIGALNLYDGLI 5E9D7F48F8AADA21 pik1_yeast Phosphatidylinositol 4-kinase PIK1 N0795 YNL267W PIK1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MHKASSSKKSFDDTIELKKNEQLLKLINSSEFTLHNCVELLCKHSENIGIHYYLCQKLATFPHSELQFYIPQLVQVLVTMETESMALEDLLLRLRAENPHFALLTFWQLQALLTDLSTDPASYGFQVARRVLNNLQTNLFNTSSGSDKNVKIHENVAPALVLSSMIMSAIAFPQLSEVTKPLVESQGRRQKAFVFKLARSAMKDFTKNMTLKNTLLNKKTSRSKRVSSNRSSTPTSPIDLIDPIKTKEDASFRKSRHSEVKLDFDIVDDIGNQVFEERISSSIKLPKRKPKYLDNSYVHRTYDGKNINRDGSISNTAKALDGNKGDYISPKGRNDENNEIGNNEDETGGETEEDADALNSDHFTSSMPDLHNIQPRTSSASSASLEGTPKLNRTNSQPLSRQAFKNSKKANSSLSQEIDLSQLSTTSKIKMLKANYFRCETQFAIALETISQRLARVPTEARLSALRAELFLLNRDLPAEVDIPTLLPPNKKGKLHKLVTITANEAQVLNSAEKVPYLLLIEYLRDEFDFDPTSETNERLLKKISGNQGGLIFDLNYMNRKENNENRNESTLTSNNTRSSVYDSNSFNNGASRNEGLSSTSRSDSASTAHVRTEVNKEEDLGDMSMVKVRNRTDDEAYRNALVIQSAANVPILPDDSQDRSPELNFGSNLDEVLIENGINSKNIHSQTDALADQMRVSAVMLAQLDKSPQQLSESTKQIRAQIISSMKEVQDKFGYHDLEALHGMAGERKLENDLMTGGIDTSYLGEDWATKKERIRKTSEYGHFENWDLCSVIAKTGDDLRQEAFAYQMIQAMANIWVKEKVDVWVKRMKILITSANTGLVETITNAMSVHSIKKALTKKMIEDAELDDKGGIASLNDHFLRAFGNPNGFKYRRAQDNFASSLAAYSVICYLLQVKDRHNGNIMIDNEGHVSHIDFGFMLSNSPGSVGFEAAPFKLTYEYIELLGGVEGEAFKKFVELTKSSFKALRKYADQIVSMCEIMQKDNMQPCFDAGEQTSVQLRQRFQLDLSEKEVDDFVENFLIGKSLGSIYTRIYDQFQLITQGIYS 7666979CA14B1CB5 gga2_yeast ADP-ribosylation factor-binding protein GGA2 YHR108W GGA2 Golgi-localized, gamma ear-containing, ARF-binding protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHPHSHSIYLSELPVRKPQALGNPLLRKIQRACRMSLAEPDLALNLDIADYINEKQGAAPRDAAIALAKLINNRESHVAIFALSLLDVLVKNCGYPFHLQISRKEFLNELVKRFPGHPPLRYSKIQRLILTAIEEWYQTICKHSSYKNDMGYIRDMHRLLKYKGYAFPKISESDLAVLKPSNQLKTASEIQKEQEIAQAAKLEELIRRGKPEDLREANKLMKIMAGFKEDNAVQAKQAISSELNKLKRKADLLNEMLESPDSQNWDNETTQELHSALKVAQPKFQKIIEEEQEDDALVQDLLKFNDTVNQLLEKFNLLKNGDSNAASQIHPSHVSAPLQQSSGALTNEINLIDFNDLDEAPSQGNNNTNGTGTPAAAETSVNDLLGDLTDLSISNPSTANQASFGLGGDIVLGSSQPAPPVTTTNNSNNTLDLLGLSTPQSPTNSQAVNSSGFDLLMGFNPTTGTTTAPARTLVNQSPNLKIEFEISRESNSVIRIKSFFTNLSSSPISNLVFLLAVPKSMSLKLQPQSSNFMIGNAKDGISQEGTIENAPANPSKALKVKWKVNYSVNSTQAEETAVFTLPNV 7AC3A317AEE302E2 gga2-frq1-1 3237200 N/A 3237203 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3237204 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3237205 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false gga2-pik1-1 3237201 N/A 3237204 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 80-760 Region 80-760 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3237205 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-169 Region 1-169 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false gga2-pik1-2 3237202 N/A 3237204 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3237205 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-169 Region 1-169 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false