DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ calmettes-2012-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. DIP original experiment: DIP-250831X Nature structural & molecular biology 2012 IMEx The structural basis of transferrin sequestration by transferrin-binding protein B. Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. Only protein-protein interactions imex curation calmettes-2012-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. DIP original experiment: DIP-250832X Nature structural & molecular biology 2012 IMEx The structural basis of transferrin sequestration by transferrin-binding protein B. Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. Only protein-protein interactions imex curation calmettes-2012-3 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. DIP original experiment: DIP-250833X Nature structural & molecular biology 2012 IMEx The structural basis of transferrin sequestration by transferrin-binding protein B. Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. Only protein-protein interactions imex curation trfe_human Serotransferrin Siderophilin Beta-1 metal-binding globulin TF PRO1400 protein protein human Homo sapiens Human MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP tbpb1_neimi Transferrin-binding protein B tbpB tbp2 TbpB receptor Transferrin-binding protein 2 protein protein Neisseria meningitidis serogroup B Neisseria meningitidis serogroup B MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKYQDVSSEKPQAQKDQGGYGFAMRLKRRNWYPGAEESEVKLNESDWEATGLPTKPKELPKRQKSVIEKVETDGDSDIYSSPYLTPSNHQNGSAGNGVNQPKNQATGHENFQYVYSGWFYKHAASEKDFSNKKIKSGDDGYIFYHGEKPSRQLPASGKVIYKGVWHFVTDTKKGQDFREIIQPSKKQGDRYSGFSGDGSEEYSNKNESTLKDDHEGYGFTSNLEVDFGNKKLTGKLIRNNASLNNNTNNDKHTTQYYSLDAQITGNRFNGTATATDKKENETKLHPFVSDSSSLSGGFFGPQGEELGFRFLSDDQKVAVVGSAKTKDKLENGAAASGSTGAAASGGAAGTSSENSKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPKDSESGNTQADKGKNGGTEFTRKFEHTPESDKKDAQAGTQTNGAQTASNTAGDTNGKTKTYEVEVCCSNLNYLKYGMLTRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERTDEKEIPTDQNVVYRGSWYGHIANGTSWSGNASDKEGGNRAEFTVNFADKKITGKLTAENRQAQTFTIEGMIQGNGFEGTAKTAESGFDLDQKNTTRTPKAYITDAKVKGGFYGPKAEELGGWFAYPGDKQTEKATATSSDGNSASSATVVFGAKRQQPVQ tbpb-tf-3 3236807 N/A 3236810 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3236811 unspecified role unspecified role unspecified role unspecified role Region 56-711 Region 56-711 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tbpb-tf-4 3236808 N/A 3236810 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3236811 unspecified role unspecified role unspecified role unspecified role Region 56-711 Region 56-711 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tbpb-tf-5 3236809 N/A 3236810 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3236811 unspecified role unspecified role bait bait Region 56-711 Region 56-711 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false