DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ feldman_salit-2012-1 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249263X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions imex curation feldman_salit-2012-2 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249260X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions imex curation feldman_salit-2012-3 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249261X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions imex curation feldman_salit-2012-4 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249262X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions imex curation sat3_arath Serine acetyltransferase 3, mitochondrial SAT3 SAT1 SATA MJG19.6 At3g13110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLPVTSRRHFTMSLYMLRSSSPHINHHSFLLPSFVSSKFKHHTLSPPPSPPPPPPMAACIDTCRTGKPQISPRDSSKHHDDESGFRYMNYFRYPDRSSFNGTQTKTLHTRPLLEDLDRDAEVDDVWAKIREEAKSDIAKEPIVSAYYHASIVSQRSLEAALANTLSVKLSNLNLPSNTLFDLFSGVLQGNPDIVESVKLDLLAVKERDPACISYVHCFLHFKGFLACQAHRIAHELWTQDRKILALLIQNRVSEAFAVDFHPGAKIGTGILLDHATAIVIGETAVVGNNVSILHNVTLGGTGKQCGDRHPKIGDGVLIGAGTCILGNITIGEGAKIGAGSVVLKDVPPRTTAVGNPARLLGGKDNPKTHDKIPGLTMDQTSHISEWSDYVI cyskm_arath Cysteine synthase, mitochondrial Beta-substituted Ala synthase 2;2 OASC ACS 1 F24G16.30 At3g59760 O-acetylserine sulfhydrylase CSase C O-acetylserine (thiol)-lyase OAS-TL C protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVAMIMASRFNREAKLASQILSTLLGNRSCYTSMAATSSSALLLNPLTSSSSSSTLRRFRCSPEISSLSFSSASDFSLAMKRQSRSFADGSERDPSVVCEAVKRETGPDGLNIADNVSQLIGKTPMVYLNSIAKGCVANIAAKLEIMEPCCSVKDRIGYSMVTDAEQKGFISPGKSVLVEPTSGNTGIGLAFIAASRGYRLILTMPASMSMERRVLLKAFGAELVLTDPAKGMTGAVQKAEEILKNTPDAYMLQQFDNPANPKIHYETTGPEIWDDTKGKVDIFVAGIGTGGTITGVGRFIKEKNPKTQVIGVEPTESDILSGGKPGPHKIQGIGAGFIPKNLDQKIMDEVIAISSEEAIETAKQLALKEGLMVGISSGAAAAAAIKVAKRPENAGKLIAVVFPSFGERYLSTPLFQSIREEVEKMQPEV oasc-sat3-1 3236417 N/A 3236421 unspecified role unspecified role unspecified role unspecified role Region 355-391 Region 355-391 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3236422 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false oasc-1 3236418 N/A 3236422 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false oasc-2 3236419 N/A 3236422 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sat3-oasc-3 3236420 N/A 3236421 unspecified role unspecified role bait bait Region 387-391 Region 387-391 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3236422 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false