DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ buller-2012-1 in vitro In vitro protease assay protease assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Buller AR., Freeman MF., Wright NT., Schildbach JF., Townsend CA. DIP original experiment: DIP-251519X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Insights into cis-autoproteolysis reveal a reactive state formed through conformational rearrangement. Buller AR., Freeman MF., Wright NT., Schildbach JF., Townsend CA. Only protein-protein interactions imex curation buller-2012-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Buller AR., Freeman MF., Wright NT., Schildbach JF., Townsend CA. DIP original experiment: DIP-251520X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Insights into cis-autoproteolysis reveal a reactive state formed through conformational rearrangement. Buller AR., Freeman MF., Wright NT., Schildbach JF., Townsend CA. Only protein-protein interactions imex curation q83xn4_strct protein protein strct Streptomyces cattleya MDPEAGSRARGLKGVHRVSANSGGIGGVPGPHNGLTDVPGVRVGHAGRTGDGWLTGVTVVLAPPGGAVAAVDVRGGGPGTRETDALDPRNLVQTIDAVVLTGGSAFGLDAAGGVAAWLEEQGRGFPVGADPSQVVPVVPAAALFDLGRGGTWRARPDAALGRAAVEAAAARPEGDPVEQGGVGAGTGAVVGGLKGGIGTASVVLDSGATVAALAAVNAAGSAVDPATGVLYGARTGLPGEFAGYGVPDAIGADTHARARARLAEAAEETARRRAGGAATLNTTLAVVATDATLTRAQAQKLAGTAHDGLARAVRPVHLLSDGDTVFALSTGRRPLLPDRPDATAARAFGVHLEAGALNEVLAAGADVLTRAVVHAVLAATGVDTPGGVHPSYRELYARP q83xn4_strct-1 3236201 N/A 3236203 putative self putative self unspecified role unspecified role Q83XN4:p.Thr282Ser T282S mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q83XN4:p.Thr282Cys T282C mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q83XN4:p.Asn217Asp N217D mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q83XN4:p.Asp321Asn D321N mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q83XN4:p.Asp323Asn D323N mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli protein cleavage protein cleavage false false false q83xn4_strct-2 3236202 N/A 3236203 unspecified role unspecified role unspecified role unspecified role Q83XN4:p.Thr282Cys T282C mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false