DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kim-2012-14 in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250821X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-15 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250825X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-16 human Homo sapiens Human 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250809X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-17 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250817X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-18 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250814X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-19 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250829X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-20 human Homo sapiens Human 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250810X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-21 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250828X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-22 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250822X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-23 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250815X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-24 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250811X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-25 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250818X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-26 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250823X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-27 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250827X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-28 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250819X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-29 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250812X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-30 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250824X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-31 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250826X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-32 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250816X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-33 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250813X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation kim-2012-34 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. DIP original experiment: DIP-250820X Nature structural & molecular biology 2012 IMEx ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4. Kim MO., Kim SH., Cho YY., Nadas J., Jeong CH., Yao K., Kim DJ., Yu DH., Keum YS., Lee KY., Huang Z., Bode AM., Dong Z. Only protein-protein interactions imex curation klf4_mouse Krueppel-like factor 4 Klf4 Ezf Gklf Zie Epithelial zinc finger protein EZF Gut-enriched krueppel-like factor protein protein mouse Mus musculus Mouse MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAASNTGGGLLYSRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF mk03_human Mitogen-activated protein kinase 3 ERT2 MAP kinase isoform p44 Microtubule-associated protein 2 kinase Extracellular signal-regulated kinase 1 Insulin-stimulated MAP2 kinase p44-ERK1 MAPK3 ERK1 PRKM3 protein protein human Homo sapiens Human MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP fbw1b_human F-box/WD repeat-containing protein 11 KIAA0696 FBXW11 BTRCP2 FBW1B FBXW1B F-box/WD repeat-containing protein 1B F-box and WD repeats protein beta-TrCP2 Homologous to Slimb protein protein protein human Homo sapiens Human MEPDSVIEDKTIELMCSVPRSLWLGCANLVESMCALSCLQSMPSVRCLQISNGTSSVIVSRKRPSEGNYQKEKDLCIKYFDQWSESDQVEFVEHLISRMCHYQHGHINSYLKPMLQRDFITALPEQGLDHIAENILSYLDARSLCAAELVCKEWQRVISEGMLWKKLIERMVRTDPLWKGLSERRGWDQYLFKNRPTDGPPNSFYRSLYPKIIQDIETIESNWRCGRHNLQRIQCRSENSKGVYCLQYDDEKIISGLRDNSIKIWDKTSLECLKVLTGHTGSVLCLQYDERVIVTGSSDSTVRVWDVNTGEVLNTLIHHNEAVLHLRFSNGLMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWSTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLQAALDPRAPASTLCLRTLVEHSGRVFRLQFDEFQIISSSHDDTILIWDFLNVPPSAQNETRSPSRTYTYISR mk03_mouse Mitogen-activated protein kinase 3 Mapk3 Extracellular signal-regulated kinase 1 Insulin-stimulated MAP2 kinase p44-ERK1 ERT2 MAP kinase isoform p44 Microtubule-associated protein 2 kinase MNK1 Erk1 Prkm3 protein protein mouse Mus musculus Mouse MAAAAAAPGGGGGEPRGTAGVVPVVPGEVEVVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRAPTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFDMELDDLPKERLKELIFQETARFQPGAPEGP mk01_human Mitogen-activated protein kinase 1 MAPK1 ERK2 PRKM1 Extracellular signal-regulated kinase 2 Mitogen-activated protein kinase 2 MAP kinase isoform p42 ERT1 PRKM2 protein protein human Homo sapiens Human MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG mk01_mouse Mitogen-activated protein kinase 1 Mapk1 Erk2 Mapk Prkm1 Extracellular signal-regulated kinase 2 Mitogen-activated protein kinase 2 MAP kinase isoform p42 ERT1 protein protein mouse Mus musculus Mouse MAAAAAAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNLNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS fbxw7_human F-box/WD repeat-containing protein 7 FBXW7 FBW7 FBX30 SEL10 F-box and WD-40 domain-containing protein 7 F-box protein FBX30 Archipelago homolog SEL-10 hCdc4 protein protein human Homo sapiens Human MNQELLSVGSKRRRTGGSLRGNPSSSQVDEEQMNRVVEEEQQQQLRQQEEEHTARNGEVVGVEPRPGGQNDSQQGQLEENNNRFISVDEDSSGNQEEQEEDEEHAGEQDEEDEEEEEMDQESDDFDQSDDSSREDEHTHTNSVTNSSSIVDLPVHQLSSPFYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK klf5_mouse Krueppel-like factor 5 Intestinal-enriched krueppel-like factor Transcription factor BTEB2 Basic transcription element-binding protein 2 Klf5 Bteb2 Iklf protein protein mouse Mus musculus Mouse MPTRVLTMSARLGPLPQPPAAQDEPVFAQLKPVLGAANPARDAALFSGDDLKHAHHHPPAPPPAAGPRLPSEELVQTRCEMEKYLTPQLPPVPIISEHKKYRRDSASVVDQFFTDTEGIPYSINMNVFLPDITHLRTGLYKSQRPCVTQIKTEPVTIFSHQSESTAPPPPPAPTQALPEFTSIFSSHQTTAPPQEVNNIFIKQELPIPDLHLSVPSQQGHLYQLLNTPDLDMPSSTNQTAVMDTLNVSMAGLNPHPSAVPQTSMKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPATLPVNSPTLPPVRYNRRSNPDLEKRRIHFCDYNGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRHYRKHTGAKPFQCMVCQRSFSRSDHLALHMKRHQN fbw1b_mouse F-box/WD repeat-containing protein 11 Fbxw11 Btrcp2 F-box and WD repeats protein beta-TrCP2 F-box/WD repeat-containing protein 1B Fbw1b Fbxw1b Homologous to Slimb protein protein protein mouse Mus musculus Mouse MEPDSVIEDKTIELMCSVPRSLWLGCANLVESMCALSCLQSMPSVRCLQISNGTSSVIVSRKRPSEGNYQKEKDLCIKYFDQWSESDQVEFVEHLISRMCHYQHGHINSYLKPMLQRDFITALPEQGLDHIAENILSYLDARSLCAAELVCKEWQRVISEGMLWKKLIERMVRTDPLWKGLSERRGWDQYLFKNRPTDGPPNSFYRSLYPKIIQDIETIESNWRCGRHNLQRIQCRSENSKGVYCLQYDDDKIISGLRDNSIKIWDKSSLECLKVLTGHTGSVLCLQYDERVIVTGSSDSTVRVWDVNTGEVLNTLIHHNEAVLHLRFSNGLMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWSTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLQAALDPRAPASTLCLRTLVEHSGRVFRLQFDEFQIISSSHDDTILIWDFLNVPPSAQNETRSPSRTYTYISR fbw1a_human F-box/WD repeat-containing protein 1A BTRC BTRCP FBW1A FBXW1A F-box and WD repeats protein beta-TrCP E3RSIkappaB pIkappaBalpha-E3 receptor subunit Epididymis tissue protein Li 2a protein protein human Homo sapiens Human MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR klf4-mapk3-1 3235933 N/A 3235954 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q60793:p.Ser132Ala S132A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false pS132 pS132 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235955 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false klf4-fbxw11-1 3235934 N/A 3235956 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false klf4-mapk3-2 3235935 N/A 3235957 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3235954 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false klf4-fbxw11-2 3235936 N/A 3235956 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false klf4-mapk1-1 3235937 N/A 3235958 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3235954 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 10-149 Region 10-149 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false klf4-ub-1 3235938 N/A 3235959 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false mouse Mus musculus Mouse N/A 3235954 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q60793:p.Ser132Ala S132A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse physical association physical association false false false klf4-mapk1-2 3235939 N/A 3235960 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3235954 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false ub-klf4-1 3235940 N/A 3235959 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false mouse Mus musculus Mouse N/A 3235954 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q60793:p.Ser132Ala S132A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse physical association physical association false false false klf5-fbxw7-1 3235941 N/A 3235961 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235962 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false mapk3-klf4-1 3235942 N/A 3235957 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role prey prey Region 10-149 Region 10-149 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false mapk3-klf4-2 3235943 N/A 3235957 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role prey prey Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q60793:p.[Leu89Ala;Arg91Ala;Arg92Ala] L89A/R91A/R92A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q60793:p.[Leu101Ala;Leu103Ala] L101A/L103A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q60793:p.[Leu89Ala;Arg91Ala;Arg92Ala;Leu101Ala;Leu103Ala] L89A/R91A/R92A/L101A/L103A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false klf4-fbxw11-3 3235944 N/A 3235963 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3235954 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false fbxw7-klf5-1 3235945 N/A 3235961 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235962 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false fbxw11-klf4-1 3235946 N/A 3235956 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false ub-klf4-2 3235947 N/A 3235959 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3235954 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q60793:p.Ser132Ala S132A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false mapk3-2 3235948 N/A 3235955 putative self putative self unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false klf4-btrc-1 3235949 N/A 3235964 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false btrc-klf4-1 3235950 N/A 3235964 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false klf4-btrc-2 3235951 N/A 3235964 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 10-483 Region 10-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false klf4-mapk3-3 3235952 N/A 3235954 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 10-149 Region 10-149 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q60793:p.Ser132Ala S132A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q60793:p.[Leu89Ala;Arg91Ala;Arg92Ala] L89A/R91A/R92A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235955 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false fbxw11-klf4-2 3235953 N/A 3235956 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235954 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q60793:p.Ser132Ala S132A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q60793:p.Ser136Ala S136A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q60793:p.Ser140Ala S140A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 10-149 Region 10-149 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false