DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ du-2012-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249253X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions imex curation du-2012-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249251X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions imex curation du-2012-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249256X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions imex curation du-2012-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249254X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions imex curation du-2012-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249252X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions imex curation du-2012-6 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249257X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions imex curation du-2012-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249255X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions imex curation smacn Diablo peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) AVPIAQKSEPHSLSS birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD h3k9-2 Histone H3 N-terminal Peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLA birc5-smacn-1 3235230 N/A 3235237 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3235238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false birc5-h3(121)-1 3235231 N/A 3235239 unspecified role unspecified role unspecified role unspecified role Region 1-10 Region 1-10 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3235238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false birc5-smacn-2 3235232 N/A 3235237 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3235238 unspecified role unspecified role unspecified role unspecified role O15392:p.[Lys62Tyr;His80Trp] K62Y/H80W mutation increasing mutation increasing interaction mutation increasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc5-h3(121)-2 3235233 N/A 3235239 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false pT3 pT3 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3235238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false birc5-h3(121)-3 3235234 N/A 3235239 unspecified role unspecified role unspecified role unspecified role pT3 pT3 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3235238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false birc5-h3(121)-4 3235235 N/A 3235239 unspecified role unspecified role unspecified role unspecified role pT3 pT3 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3235238 unspecified role unspecified role unspecified role unspecified role O15392:p.Glu63Ala E63A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O15392:p.Leu64Ala L64A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O15392:p.Trp67Ala W67A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O15392:p.His80Ala H80A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O15392:p.[Lys62Tyr;His80Trp] K62Y/H80W mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O15392:p.Glu76Ala E76A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O15392:p.Asp71Ala D71A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O15392:p.Glu65Ala E65A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3(121)-birc5-1 3235236 N/A 3235239 unspecified role unspecified role bait bait Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3235238 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false