DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ xie-2012-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249250X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions imex curation xie-2012-5 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249244X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions imex curation xie-2012-6 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249248X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions imex curation xie-2012-7 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249247X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions imex curation xie-2012-8 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249246X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions imex curation xie-2012-9 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249245X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions imex curation xie-2012-10 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249249X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions imex curation phf12_mouse PHD finger protein 12 Phf12 Kiaa1523 PHD factor 1 protein protein mouse Mus musculus Mouse MWEKMETKTIVYDLDTSGGLMEQIQALLAPPKTDEAEKRSRKPEKESRRSGRATNHDSCDSCKEGGDLLCCDHCPAAFHLQCCNPPLSEEMLPPGEWMCHRCTVRRKKREQKKELGHVNGLVDKSSKRTTSPSSDTDLLDRPASKTELKAIAHARILERRASRPGTPTSNASTETPTSEHNDVDEDIVDVDEEPVAAEPDYVQPQLRRPFELLIAAAMERNPTQFQLPNELTCTTALPGSSKRRRKEETTGKNVKRTQHELDHNGLVPLPVKVCFTCNRSCRVAPLIQCDYCPLLFHMDCLEPPLTAMPLGRWMCPNHIEHVVLNQKNLTLSNRCQVFDRFQDTISQHVVKVDFLNRIHKKHPPNRRVLQSVKRRSLKVPDAIKSQYQFPPPLIAPAAIRDGELICSGVPEESQTHLLNSEHLATQAEQQEWLCSVVALQCSILKHLSAKQMPSPWDSEQTEKADIKPVIVTDSSITTSLQTADKAPLPSHYPLSCPSAVSTQNSLGCSPPHQPPTLEDISCSSCVEKSKKAPCGTANGPVNTEIKANGPHLYSSPTDSTDPRRLPGANTPLPGLTHRQGWPRPLTPPSAGGLQNHVGIIVKTENATGPSSCPQRSLVPVPSLPPSIPSSCASIENTSTLHRKTVQSQIGPSSTESRPLGSPPNATRVLTPPQAAGDSILATGANQRFCSPAPSSDGKVSPGTLSIGSALTVPSFPANSTAMVDLTNSLRAFMDVNGEIEINMLDEKLIKFLALQRVHQLFPSRVQASPGNVGTHPLASGGHHPEVQRKEVQARAVFCPLLGLGGAVNMCYRTLYIGTGADMDVCLTNYGHCNYVSGKHACIFYDENTKHYELLNYSEHGTTVDNVLYSCDFSEKTPPTPPSSIVAKVQSVIRRRRHQKQDEEPSEEAAMMSSQAQGPQRRPCNCKASSSSLIGGSGAGWEGTALLHHGSYIKLGCLQFVFSITEFATKQPKGDASLLQDGVLAEKLSLKPHQGPVLRSNSVP q9ubu8-2 Mortality factor 4-like protein 1 MORF4L1 MORF-related gene 15 protein MRG15 Protein MSL3-1 Transcription factor-like protein MRG15 FWP006 HSPC008 HSPC061 PP368 protein protein human Homo sapiens Human MAPKQDPKPKFQEGERVLCFHGPLLYEAKCVKVAIKDKQVKYFIHYSGWNKNWDEWVPESRVLKYVDTNLQKQRELQKANQEQYAEGKMRGAAPGKKTSGLQQKNVEVKTKKNKQKTPGNGDGGSTSETPQPPRKKRARVDPTVENEETFMNRVEVKVKIPEELKPWLVDDWDLITRQKQLFYLPAKKNVDSILEDYANYKKSRGNTDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEILADHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLNYLHDFLKYLAKNSATLFSASDYEVAPPEYHRKAV phf12_human PHD finger protein 12 KIAA1523 PHD factor 1 PHF12 protein protein human Homo sapiens Human MWEKMETKTIVYDLDTSGGLMEQIQALLAPPKTDEAEKRSRKPEKEPRRSGRATNHDSCDSCKEGGDLLCCDHCPAAFHLQCCNPPLSEEMLPPGEWMCHRCTVRRKKREQKKELGHVNGLVDKSGKRTTSPSSDTDLLDRSASKTELKAIAHARILERRASRPGTPTSSASTETPTSEQNDVDEDIIDVDEEPVAAEPDYVQPQLRRPFELLIAAAMERNPTQFQLPNELTCTTALPGSSKRRRKEETTGKNVKKTQHELDHNGLVPLPVKVCFTCNRSCRVAPLIQCDYCPLLFHMDCLEPPLTAMPLGRWMCPNHIEHVVLNQKNMTLSNRCQVFDRFQDTVSQHVVKVDFLNRIHKKHPPNRRVLQSVKRRSLKVPDAIKSQYQFPPPLIAPAAIRDGELICNGIPEESQMHLLNSEHLATQAEQQEWLCSVVALQCSILKHLSAKQMPSHWDSEQTEKADIKPVIVTDSSVTTSLQTADKTPTPSHYPLSCPSGISTQNSLSCSPPHQSPALEDIGCSSCAEKSKKTPCGTANGPVNTEVKANGPHLYSSPTDSTDPRRLPGANTPLPGLSHRQGWPRPLTPPAAGGLQNHTVGIIVKTENATGPSSCPQRSLVPVPSLPPSIPSSCASIENTSTLQRKTVQSQIGPPLTDSRPLGSPPNATRVLTPPQAAGDGILATTANQRFSSPAPSSDGKVSPGTLSIGSALTVPSFPANSTAMVDLTNSLRAFMDVNGEIEINMLDEKLIKFLALQRIHQLFPSRVQPSPGSVGTHQLASGGHHIEVQRKEVQARAVFYPLLGLGGAVNMCYRTLYIGTGADMDVCLTNYGHCNYVSGKHACIFYDENTKHYELLNYSEHGTTVDNVLYSCDFSEKTPPTPPSSIVAKVQSVIRRRRHQKQDEEPSEEAAMMSSQAQGPQRRPCNCKASSSSLIGGSGAGWEGTALLHHGSYIKLGCLQFVFSITEFATKQPKGDASLLQDGVLAEKLSLKPHQGPVLRSNSVP palb2_human Partner and localizer of BRCA2 PALB2 FANCN protein protein human Homo sapiens Human MDEPPGKPLSCEEKEKLKEKLAFLKREYSKTLARLQRAQRAEKIKHSIKKTVEEQDCLSQQDLSPQLKHSEPKNKICVYDKLHIKTHLDEETGEKTSITLDVGPESFNPGDGPGGLPIQRTDDTQEHFPHRVSDPSGEQKQKLPSRRKKQQKRTFISQERDCVFGTDSLRLSGKRLKEQEEISSKNPARSPVTEIRTHLLSLKSELPDSPEPVTEINEDSVLIPPTAQPEKGVDTFLRRPNFTRATTVPLQTLSDSGSSQHLEHIPPKGSSELTTHDLKNIRFTSPVSLEAQGKKMTVSTDNLLVNKAISKSGQLPTSSNLEANISCSLNELTYNNLPANENQNLKEQNQTEKSLKSPSDTLDGRNENLQESEILSQPKSLSLEATSPLSAEKHSCTVPEGLLFPAEYYVRTTRSMSNCQRKVAVEAVIQSHLDVKKKGFKNKNKDASKNLNLSNEETDQSEIRMSGTCTGQPSSRTSQKLLSLTKVSSPAGPTEDNDLSRKAVAQAPGRRYTGKRKSACTPASDHCEPLLPTSSLSIVNRSKEEVTSHKYQHEKLFIQVKGKKSRHQKEDSLSWSNSAYLSLDDDAFTAPFHRDGMLSLKQLLSFLSITDFQLPDEDFGPLKLEKVKSCSEKPVEPFESKMFGERHLKEGSCIFPEELSPKRMDTEMEDLEEDLIVLPGKSHPKRPNSQSQHTKTGLSSSILLYTPLNTVAPDDNDRPTTDMCSPAFPILGTTPAFGPQGSYEKASTEVAGRTCCTPQLAHLKDSVCLASDTKQFDSSGSPAKPHTTLQVSGRQGQPTCDCDSVPPGTPPPIESFTFKENQLCRNTCQELHKHSVEQTETAELPASDSINPGNLQLVSELKNPSGSCSVDVSAMFWERAGCKEPCIITACEDVVSLWKALDAWQWEKLYTWHFAEVPVLQIVPVPDVYNLVCVALGNLEIREIRALFCSSDDESEKQVLLKSGNIKAVLGLTKRRLVSSSGTLSDQQVEVMTFAEDGGGKENQFLMPPEETILTFAEVQGMQEALLGTTIMNNIVIWNLKTGQLLKKMHIDDSYQASVCHKAYSEMGLLFIVLSHPCAKESESLRSPVFQLIVINPKTTLSVGVMLYCLPPGQAGRFLEGDVKDHCAAAILTSGTIAIWDLLLGQCTALLPPVSDQHWSFVKWSGTDSHLLAGQKDGNIFVYHYS phf12-morf4l1-2 3235161 N/A 3235168 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3235169 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UBU8-2:p.Val227Ala V227A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Leu287Ala L287A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Leu168Ala L168A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false morf4l1-phf12-1 3235162 N/A 3235170 unspecified role unspecified role unspecified role unspecified role Region 200-241 Region 200-241 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235169 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-phf12-2 3235163 N/A 3235170 unspecified role unspecified role unspecified role unspecified role Region 219-232 Region 219-232 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3235169 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-palb2-2 3235164 N/A 3235171 unspecified role unspecified role unspecified role unspecified role Region 562-629 Region 562-629 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3235169 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-phf12-3 3235165 N/A 3235170 unspecified role unspecified role unspecified role unspecified role Region 200-241 Region 200-241 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q96QT6:p.Phe210Ala F210A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96QT6:p.Phe225Ala F225A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96QT6:p.Leu213Ala L213A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96QT6:p.Leu227Ala L227A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96QT6:p.Pro228Ala P228A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96QT6:p.Asn221Glu N221E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235169 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-phf12-4 3235166 N/A 3235170 unspecified role unspecified role unspecified role unspecified role Region 200-241 Region 200-241 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235169 unspecified role unspecified role unspecified role unspecified role Q9UBU8-2:p.Val268Ala V268A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Val227Ala V227A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Leu287Ala L287A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Tyr235Ala Y235A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Met228Ala M228A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Met273Ala M273A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Trp172Ala W172A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false phf12-morf4l1-3 3235167 N/A 3235168 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q5SPL2:p.Phe210Ala F210A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q5SPL2:p.Phe225Ala F225A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q5SPL2:p.Leu213Ala L213A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q5SPL2:p.Leu227Ala L227A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q5SPL2:p.Pro228Ala P228A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3235169 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false