DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pathare-2012-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pathare GR., Nagy I., Bohn S., Unverdorben P., Hubert A., Körner R., Nickell S., Lasker K., Sali A., Tamura T., Nishioka T., Förster F., Baumeister W., Bracher A. DIP original experiment: DIP-251456X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx The proteasomal subunit Rpn6 is a molecular clamp holding the core and regulatory subcomplexes together. Pathare GR., Nagy I., Bohn S., Unverdorben P., Hubert A., Körner R., Nickell S., Lasker K., Sali A., Tamura T., Nishioka T., Förster F., Baumeister W., Bracher A. Only protein-protein interactions imex curation q7klv9-1 26S proteasome non-ATPase regulatory subunit 11 Rpn6 26S proteasome regulatory complex subunit p42B 26S proteasome regulatory subunit Rpn6 CG10149 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAGATLFERAQALSSVNREEQDSSLLNKLVRDQEGAENDEERIRIKEQGILQQGELYKQEGKAKELADLIKVTRPFLSSISKAKAAKLVRSLVDMFLDMDAGTGIEVQLCKDCIEWAKQEKRTFLRQSLEARLIALYFDTALYTEALALGAQLLRELKKLDDKNLLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKVQGALDLQSGILHAADERDFKTAFSYFYEAFEGFDSVDSVKALTSLKYMLLCKIMLGQSDDVNQLVSGKLAITYSGRDIDAMKSVAEASHKRSLADFQAALKEYKKELAEDVIVQAHLGTLYDTMLEQNLCRIIEPYSRVQVAHVAESIQLPMPQVEKKLSQMILDKKFSGILDQGEGVLIVFEETPVDKTYERVLETIQSMGKVVDTLYQKAKKLS psmd6_drome 26S proteasome non-ATPase regulatory subunit 6 Rpn7 CG5378 26S proteasome regulatory subunit S10 Protein Rpn7 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPAENLEEQGLEKNPNLELAQTKFLLTLAEYKQDAALKAKLLEAIRTENMAPWYEHICSELGWTVDKDLLARMKENNRVEVEQLDAAIEDAEKNLGEMEVREANLKKSEYLCRIGDKAAAETAFRKTYEKTVSLGHRLDIVFHLIRLGLFYLDHDLITRNIDKAKYLIEEGGDWDRRNRLKVYQGVYSVAVRDFKAAATFFLDTVSTFTSYELMDYPTFVRYTVYVAMIALPRNELRDKVIKGSEIQEVLHGLPDVKQFLFSLYNCQYENFYVHLAGVEKQLRLDYLIHPHYRYYVREMRILGYTQLLESYRSLTLQYMAESFGVTVEYIDQELARFIAAGRLHAKVDRVGGIVETNRPDNKNWQYQATIKQGDLLLNRIQKLSRVINI rpn7-rpn6-3 3234535 N/A 3234536 unspecified role unspecified role prey prey Region 30-422 Region 30-422 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 391-422 Region 391-422 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q7KLV9-1:p.[Ser366Lys;Ile369Ala;Leu370Ala] S366K/I369A/L370A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rhoer Rhodococcus erythropolis Arthrobacter picolinophilus N/A 3234537 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false rhoer Rhodococcus erythropolis Arthrobacter picolinophilus direct interaction direct interaction false false false