DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sitar-2011-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253817X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions imex curation sitar-2011-2 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253819X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions imex curation sitar-2011-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253818X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions imex curation sitar-2011-4 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253820X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions imex curation sitar-2011-5 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253822X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions imex curation sitar-2011-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253821X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions imex curation act1_drome Actin-5C Act5C CG4027 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCDEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF q9u1k1-1 Protein spire p150-Spir CG10076 spir protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTMEPAKQVPPPEEPSFTKDEYHKFYDTALESYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF act5c-spir-4 3233012 N/A 3233018 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3233019 unspecified role unspecified role unspecified role unspecified role Region 428-485 Region 428-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-spir-5 3233013 N/A 3233020 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3233019 unspecified role unspecified role unspecified role unspecified role Region 428-485 Region 428-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 396-450 Region 396-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-spir-6 3233014 N/A 3233020 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3233019 unspecified role unspecified role unspecified role unspecified role Region 428-485 Region 428-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 396-450 Region 396-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false act5c-spir-5 3233015 N/A 3233018 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3233019 unspecified role unspecified role unspecified role unspecified role Region 396-485 Region 396-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-spir-7 3233016 N/A 3233020 unspecified role unspecified role unspecified role unspecified role Pyrene-label Pyrene-label pyrene label pyrene label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3233019 unspecified role unspecified role unspecified role unspecified role Region 448-485 Region 448-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false act5c-spir-6 3233017 N/A 3233018 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3233019 unspecified role unspecified role unspecified role unspecified role Region 371-388 Region 371-388 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 396-419 Region 396-419 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 428-450 Region 428-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 461-485 Region 461-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false