DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ schalch-2011-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. DIP original experiment: DIP-255463X Nature structural & molecular biology 2011 IMEx The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. Only protein-protein interactions imex curation schalch-2011-2 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. DIP original experiment: DIP-255459X Nature structural & molecular biology 2011 IMEx The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. Only protein-protein interactions imex curation schalch-2011-3 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. DIP original experiment: DIP-255457X Nature structural & molecular biology 2011 IMEx The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. Only protein-protein interactions imex curation schalch-2011-4 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. DIP original experiment: DIP-255461X Nature structural & molecular biology 2011 IMEx The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. Only protein-protein interactions imex curation schalch-2011-5 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. DIP original experiment: DIP-255462X Nature structural & molecular biology 2011 IMEx The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. Only protein-protein interactions imex curation schalch-2011-6 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. DIP original experiment: DIP-255460X Nature structural & molecular biology 2011 IMEx The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. Only protein-protein interactions imex curation schalch-2011-7 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm light scattering light scattering predetermined predetermined participant predetermined Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. DIP original experiment: DIP-255458X Nature structural & molecular biology 2011 IMEx The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Schalch T., Job G., Shanker S., Partridge JF., Joshua-Tor L. Only protein-protein interactions imex curation tas3_schpo RNA-induced transcriptional silencing complex protein tas3 SPBC83.03c tas3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEKGIKKYLPSLPFLACISDFPENHGTSRRSATVSLERVHELFTEHWLSNLKNRREKRQELAEEAVYCRSEMLSQRKLLAAVDFPQQLKNSPAKAKATHTSSGVTKEVRASKKYTSSNVEFPLVTDGKEKPVKSKQLRKNSVTEFEKPIETKKSKHRKSRNKFLDKSSGSMEIESWDNSTSDSIIESSSRLHESISLRENDIRSSDSKSVGWDDNSTGFRESSKSLDHTDTSMFMELDSNSDPQFRPKYQAKSSWFAPDDPEASWGNLDDGWGETNGSWSSTDDTKHYKNEWAESINLDNFNRPSQQEDYDKPKNTQVSRSSNHHRRYDSYHPDSRSDSYRSKREHYDNRDTGPRSKHLEKSSYVYNQNFEDRTHLSDHGAHFHLGNANDFNMQGSSRKRKASDRQRESRENELPTKKLNASDSHNPLASLTTDKNDLYINWLKSLSFFQTNSSCAEALVKVIPHYHNKLIDFSQVLQLVFSASEKFPIQENQPLPEQLMFLSNLEKQTPFAKAVGSSIYKLVTGKNLSLDFASQILKEASILEHKNEK 4569CF59851A22A0 chp1_schpo Chromo domain-containing protein 1 chp1 SPAC18G6.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVSVKPLPDIDSNEGETDADVYEVEDILADRVNKNGINEYYIKWAGYDWYDNTWEPEQNLFGAEKVLKKWKKRKKLIAKGLLEPFDAEDNEAKKMKREKEILRQQRQKRKSELTQLSQKVKEKFKKMRKKPARRIVTIANDEEEEDDQTMDEDAFERKSMQGELKERNLTDKTSTLSTSFGETSPDVNPFYLSEWPTVTDSILLSKSLSSDAIPLKNGEIKSTMLMPSDSDNSVPGIQNSNNLENTGAFVENANSPQSNTPLSTFRHSSPLSLSPVITSDNDVANSLFFSNSTPLPSSLKIKKEAPKLETHTILVSDNSGSLTKQDILSYFAFIKGNIEVFFLKSPKKDKVCNMAYIQFDSIEQAKLAYDKGHPSWHVTLVKGKISTDMEECKVSKSILKTTPSKKANARSVSFTQTTTDTLSESEKFASNVDLDENFDFNVNVTNEDAKQLKKSVIGSSWTTVNNDWNSVSKSDQTFENDGASKVVPAGNITLNSDNSLHHSISESEDLSSASTLSDYFRFVLRVGKSLYYAGELSFDISKLKAETEHQQLLRSLVSCKQVDVLRFVTSQYLEVFGTCLTKVLSGSLCIRSDVDMTHFKNILNRGNGAGIVLGSNYTLLLFTEDNNALMNLYDCQGQSNSPFWMVIFEPLESILVEWSAKNLRPKKPYHKSQSYLSYLLQLGHIDLHKIGAFQATQILIVSKQPSPEAEELEDTFREAAIPTFRGLEIPESLFLSQNVFVFLNVSLEDDFDQLQFLTLAKRKSCKFFLFGLSLPLKSPNDSHVGTDFKKNNEPLDKLTYSQYLRPMFPKGGVVSVTLSALIKTPRLLELISPFLEIKKDSWILILPPSIVDMVKSYFVTNNPDKSLLEIQNLLNTLQRYLTNPALKNVTLYQDWDIVIDDSADVSLASTLQLYQKKNYDKYRRFVLIHELKNELTPVNGLDIVDYDEFKETFMRAIGLK E92C16BBD0D5957C chp1-tas3-1 3232599 N/A 3232606 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3232607 unspecified role unspecified role bait bait SPOCdomain Region 504-661 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 534-960 Region 534-960 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false chp1-tas3-2 3232600 N/A 3232606 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 9-83 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3232607 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 504-960 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false chp1-tas3-3 3232601 N/A 3232606 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 9-83 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3232607 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 504-960 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false chp1-tas3-4 3232602 N/A 3232606 unspecified role unspecified role prey prey N-terminal domain Region 9-83 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3232607 unspecified role unspecified role bait bait C-terminal domain Region 504-960 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false one-strep-tag one-strep-tag one-strep-tag one-strep-tag twin-strep-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false tas3-chp1-1 3232603 N/A 3232606 unspecified role unspecified role bait bait N-terminal domain Region 9-83 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false one-strep-tag one-strep-tag one-strep-tag one-strep-tag twin-strep-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3232607 unspecified role unspecified role prey prey C-terminal domain Region 504-960 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false chp1-tas3-5 3232604 N/A 3232606 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 9-83 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3232607 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 504-960 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false chp1-tas3-6 3232605 N/A 3232606 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 9-83 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3232607 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 504-960 binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false