DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ goloudina-2012-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253541X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions imex curation goloudina-2012-2 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253544X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions imex curation goloudina-2012-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253542X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions imex curation goloudina-2012-4 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253545X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions imex curation goloudina-2012-5 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253543X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions imex curation runx2_human Runt-related transcription factor 2 Oncogene AML-3 Polyomavirus enhancer-binding protein 2 alpha A subunit SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core-binding factor alpha A subunit Osteoblast-specific transcription factor 2 Core-binding factor subunit alpha-1 Acute myeloid leukemia 3 protein RUNX2 AML3 CBFA1 OSF2 PEBP2A protein protein human Homo sapiens Human MASNSLFSTVTPCQQNFFWDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSTLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSGSYQFPMVPGGDRSPSRMLPPCTTTSNGSTLLNPNLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY ppm1d_human Protein phosphatase 1D Protein phosphatase 2C isoform delta p53-induced protein phosphatase 1 Protein phosphatase magnesium-dependent 1 delta PPM1D WIP1 protein protein human Homo sapiens Human MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPTAEEKPSPRRSLSQPLPPRPSPAALPGGEVSGKGPAVAAREARDPLPDAGASPAPSRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHKPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLEEDPWPRVNSKDHIPALVRSNAFSENFLEVSAEIARENVQGVVIPSKDPEPLEENCAKALTLRIHDSLNNSLPIGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRKTVCVC atm1981ps ATM peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) AFEEGSQSTTIGY ppm1d-runx2-1 3232509 N/A 3232514 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3232515 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false atm1981ps-ppm1d-1 3232510 N/A 3232516 enzyme target enzyme target substrate substrate unspecified role unspecified role pS6 pS6 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3232515 enzyme enzyme unspecified role unspecified role Catalytic domain Region 1-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false runx2-ppm1d-1 3232511 N/A 3232514 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3232515 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false ppm1d-runx2-2 3232512 N/A 3232514 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 425-437 Region 425-437 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pS432 pS432 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3232515 enzyme enzyme unspecified role unspecified role Catalytic domain Region 1-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false runx2-ppm1d-2 3232513 N/A 3232514 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3232515 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false