DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2011-13 in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255450X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-14 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255437X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-15 human Homo sapiens Human pull down pull down western blot western blot Immuno blot Immuno blot Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255446X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-16 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255455X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-17 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255445X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-18 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255442X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-19 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255438X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-20 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255451X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-21 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255454X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-22 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255439X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-23 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255452X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-24 human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255443X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-25 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255447X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-26 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255440X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-27 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255444X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-28 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255453X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-29 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255449X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-30 in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay western blot western blot Immuno blot Immuno blot Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255448X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-31 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255441X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation lee-2011-32 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. DIP original experiment: DIP-255456X Nature structural & molecular biology 2011 IMEx Bilateral inhibition of HAUSP deubiquitinase by a viral interferon regulatory factor protein. Lee HR., Choi WC., Lee S., Hwang J., Hwang E., Guchhait K., Haas J., Toth Z., Jeon YH., Oh TK., Kim MH., Jung JU. Only protein-protein interactions imex curation ubp8_human Ubiquitin carboxyl-terminal hydrolase 8 Ubiquitin-specific-processing protease 8 Deubiquitinating enzyme 8 USP8 KIAA0055 UBPY Ubiquitin isopeptidase Y Ubiquitin thioesterase 8 protein protein human Homo sapiens Human MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSILGPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEKGAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQIGTTLRSLKDALFKWESKTVLRNEPLVLEGGYENWLLCYPQYTTNAKVTPPPRRQNEEVSISLDFTYPSLEESIPSKPAAQTPPASIEVDENIELISGQNERMGPLNISTPVEPVAASKSDVSPIIQPVPSIKNVPQIDRTKKPAVKLPEEHRIKSESTNHEQQSPQSGKVIPDRSTKPVVFSPTLMLTDEEKARIHAETALLMEKNKQEKELRERQQEEQKEKLRKEEQEQKAKKKQEAEENEITEKQQKAKEEMEKKESEQAKKEDKETSAKRGKEITGVKRQSKSEHETSDAKKSVEDRGKRCPTPEIQKKSTGDVPHTSVTGDSGSGKPFKIKGQPESGILRTGTFREDTDDTERNKAQREPLTRARSEEMGRIVPGLPSGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKAKPQIPAERDREPSKLKRSYSSPDITQAIQEEEKRKPTVTPTVNRENKPTCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLNKADNRKRYKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNNLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVKSSAAYILFYTSLGPRVTDVAT ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG f5h9d8_hhv8 vIRF-4 VIRF-4 HHV8GK18_gp63 protein protein hhv8 Human herpesvirus 8 (HHV-8) Kaposi's sarcoma-associated herpesvirus HHV-8 MPKAGGSEWATLWIIDALENNKFPYFSWFDRNNLLFAAPAPLPAGSDIPPGWYSVYHAFDEECDRVYGPSPVVGQTVYGRFGRLLRGTRRAVVRNDLRYSDTFGGSYVVWQLVRTPFKNCTYCYGAAYGPEKLQRFIQCLLSPPMQTTATRRSDTREQSYEEAGAAAPAPPKAPSGLRGRPRKSNRYYNVGDITTEQKAACSVWIPVNEGASTSGMGSSGTRQVTQASSFTWRVPGDPPAPSTLTGPSDPHSSGAGLPGTAPPKPQHETRLAGTVSGVSGVAQTPGDTGQLAPPMRDGSRLPSTSPWIPACFPWGDLPVTGWWPQGASGLPEKVHPPTTGQFDPLSPRWTYTGIPSSQLNPAAPSWIPPHAQAGTFVGEFSQGAPLAPQGLLPQSGQCASAWLPRRETGAEGACGASTEGRAPQGAASERVYPFEPQPPSAPAPGYAKPSCYNWSPLAEPPATRPIRAPVWHPPVGHAVVPEVRTPLWIPWSSGGAPNQGLSHTQGGASATPSAGAPPTPEVAERQEPSSSGIPYVCQGDNMATGYRRVTTSSGALEVEIIDLTGDSDTPSTTVASTPLPVSGPRVFQPTVLYSAPEPAVNPEVSHLPTELERRECVCPGSGERPRVPLVSTYAGDRYAVGGYGPEQSLVPPPLGLPLTLSNLQGEDICTWEEGLGNILSELQEEPSSSTRQATDRRRPRSRSPHGRRTPVSHSGPEKPPSKMFFDPPDSQRVSFVVEIFVYGNLRGTLRREGDAGEAMLCSWPVGDTLGHLCQSFVPELLRIPRLTVPSPEQMEILNRVFEGLGHGFPIFCSMSGIYSRNATQVEGWWFGNPNSRYERILRSFSPRVPQQLFNTARYLATTAAIPQTPLSVNPVTCGTVFFGASPASTENFQNVPLTVKIFIGSIWDSLH ubp7_human Ubiquitin carboxyl-terminal hydrolase 7 USP7 HAUSP Ubiquitin thioesterase 7 Ubiquitin-specific-processing protease 7 Deubiquitinating enzyme 7 Herpesvirus-associated ubiquitin-specific protease protein protein human Homo sapiens Human MNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP ebna1-1 EBNA1 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EQGPADDPGEGPSTG ub-usp8-2 3232268 N/A 3232288 enzyme enzyme unspecified role unspecified role N/A 3232289 enzyme target enzyme target substrate substrate unspecified role unspecified role deubiquitination deubiquitination reaction deubiquitination false false false usp7-virf_4-1 3232269 N/A 3232290 unspecified role unspecified role unspecified role unspecified role Region 153-256 Region 153-256 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false virf_4-usp7-1 3232270 N/A 3232291 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3232290 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 202-216 Region 202-216 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 220-236 Region 220-236 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false usp7-virf_4-2 3232271 N/A 3232290 unspecified role unspecified role unspecified role unspecified role Region 153-256 Region 153-256 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false Deletion of region 202-216 decreased the interaction rate/strength. Deletion of region 202-216 and 237-256 decreased the interaction rate/strength. tp53-ub-14 3232272 N/A 3232289 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3232292 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false virf_4-usp7-2 3232273 N/A 3232291 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232290 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-305 Region 1-305 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 153-911 Region 153-911 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false usp7-virf_4-3 3232274 N/A 3232290 unspecified role unspecified role unspecified role unspecified role Region 153-256 Region 153-256 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tp53-usp7-12 3232275 N/A 3232292 unspecified role unspecified role unspecified role unspecified role Region 350-364 Region 350-364 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Region 355-369 Region 355-369 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false usp7-virf_4-4 3232276 N/A 3232290 unspecified role unspecified role unspecified role unspecified role Region 202-216 Region 202-216 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false usp7-virf_4-5 3232277 N/A 3232290 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3232291 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false mdm2-usp7-15 3232278 N/A 3232293 unspecified role unspecified role unspecified role unspecified role Region 138-152 Region 138-152 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false virf_4-usp7-3 3232279 N/A 3232291 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232290 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 153-256 Region 153-256 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false usp7-virf_4-6 3232280 N/A 3232291 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232290 unspecified role unspecified role prey prey V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region Δ202-256 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false virf_4-usp7-4 3232281 N/A 3232291 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3232290 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false usp7-virf_4-7 3232282 N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3232290 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ebna1-usp7-6 3232283 N/A 3232294 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false usp7-virf_4-8 3232284 N/A 3232290 unspecified role unspecified role unspecified role unspecified role Region 153-216 Region 153-216 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3232291 unspecified role unspecified role unspecified role unspecified role TRAF domain Region 62-205 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ub-usp7-3 3232285 N/A 3232289 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 3232291 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm deubiquitination deubiquitination reaction deubiquitination false false false virf_4-usp7-5 3232286 N/A 3232291 unspecified role unspecified role prey prey TRAF domain Region 62-205 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232290 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false mdm2-ub-2 3232287 N/A 3232289 unspecified role unspecified role unspecified role unspecified role N/A 3232293 unspecified role unspecified role unspecified role unspecified role direct interaction direct interaction false false false