DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ roumestand-2011-1 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. DIP original experiment: DIP-220108X Structure (London, England : 1993) 2011 IMEx Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase. Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. Only protein-protein interactions imex curation roumestand-2011-2 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. DIP original experiment: DIP-220104X Structure (London, England : 1993) 2011 IMEx Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase. Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. Only protein-protein interactions imex curation roumestand-2011-3 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. DIP original experiment: DIP-220105X Structure (London, England : 1993) 2011 IMEx Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase. Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. Only protein-protein interactions imex curation roumestand-2011-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. DIP original experiment: DIP-220107X Structure (London, England : 1993) 2011 IMEx Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase. Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. Only protein-protein interactions imex curation roumestand-2011-5 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. DIP original experiment: DIP-220106X Structure (London, England : 1993) 2011 IMEx Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase. Roumestand C., Leiba J., Galophe N., Margeat E., Padilla A., Bessin Y., Barthe P., Molle V., Cohen-Gonsaud M. Only protein-protein interactions imex curation pknb_myctu Serine/threonine-protein kinase PknB pknB MTCY10H4.14c Rv0014c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETPAGPLPYIVMEYVDGVTLRDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIHRDVKPANIMISATNAVKVMDFGIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPSARHEGLSADLDAVVLKALAKNPENRYQTAAEMRADLVRVHNGEPPEAPKVLTDAERTSLLSSAAGNLSGPRTDPLPRQDLDDTDRDRSIGSVGRWVAVVAVLAVLTVVVTIAINTFGGITRDVQVPDVRGQSSADAIATLQNRGFKIRTLQKPDSTIPPDHVIGTDPAANTSVSAGDEITVNVSTGPEQREIPDVSTLTYAEAVKKLTAAGFGRFKQANSPSTPELVGKVIGTNPPANQTSAITNVVIIIVGSGPATKDIPDVAGQTVDVAQKNLNVYGFTKFSQASVDSPRPAGEVTGTNPPAGTTVPVDSVIELQVSKGNQFVMPDLSGMFWVDAEPRLRALGWTGMLDKGADVDAGGSQHNRVVYQNPPAGTGVNRDGIITLRFGQ 6C27EEBE9D5A453B fhaa_myctu FHA domain-containing protein FhaA fhaA TB39.8 Rv0020c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MGSQKRLVQRVERKLEQTVGDAFARIFGGSIVPQEVEALLRREAADGIQSLQGNRLLAPNEYIITLGVHDFEKLGADPELKSTGFARDLADYIQEQGWQTYGDVVVRFEQSSNLHTGQFRARGTVNPDVETHPPVIDCARPQSNHAFGAEPGVAPMSDNSSYRGGQGQGRPDEYYDDRYARPQEDPRGGPDPQGGSDPRGGYPPETGGYPPQPGYPRPRHPDQGDYPEQIGYPDQGGYPEQRGYPEQRGYPDQRGYQDQGRGYPDQGQGGYPPPYEQRPPVSPGPAAGYGAPGYDQGYRQSGGYGPSPGGGQPGYGGYGEYGRGPARHEEGSYVPSGPPGPPEQRPAYPDQGGYDQGYQQGATTYGRQDYGGGADYTRYTESPRVPGYAPQGGGYAEPAGRDYDYGQSGAPDYGQPAPGGYSGYGQGGYGSAGTSVTLQLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLADLNSTNGTTVNNAPVQEWQLADGDVIRLGHSEIIVRMH 2642F9CF23FE7906 pknb-rv0020c-1 3231428 N/A 3231433 enzyme enzyme unspecified role unspecified role Region 1-331 Region 1-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231434 enzyme target enzyme target substrate substrate unspecified role unspecified role pT116 pT116 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt nmr nuclear magnetic resonance NMR nmr NMR certain certain sequence position certain certain certain sequence position certain false N-terminal domain Region 1-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false pknb-rv0020c-2 3231429 N/A 3231433 enzyme enzyme unspecified role unspecified role Region 1-331 Region 1-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231434 enzyme target enzyme target substrate substrate unspecified role unspecified role pT116 pT116 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false P71590:p.Thr116Ala T116A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false pknb-4 3231430 N/A 3231433 putative self putative self unspecified role unspecified role Region 1-331 Region 1-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false pknb-rv0020c-3 3231431 N/A 3231433 unspecified role unspecified role unspecified role unspecified role Region 286-318 Region 286-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3231434 unspecified role unspecified role unspecified role unspecified role Region 430-527 Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pknb-rv0020c-4 3231432 N/A 3231433 unspecified role unspecified role unspecified role unspecified role Region 1-331 Region 1-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P9WI81:p.Thr294Ala T294A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P9WI81:p.Thr309Ala T309A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231434 unspecified role unspecified role unspecified role unspecified role Atto647-label Atto647-label atto label atto label atto label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 430-527 Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false