DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ takeda-2011-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Takeda K., Tonthat NK., Glover T., Xu W., Koonin EV., Yanagida M., Schumacher MA. DIP original experiment: DIP-251311X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8. Takeda K., Tonthat NK., Glover T., Xu W., Koonin EV., Yanagida M., Schumacher MA. Only protein-protein interactions imex curation takeda-2011-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Takeda K., Tonthat NK., Glover T., Xu W., Koonin EV., Yanagida M., Schumacher MA. DIP original experiment: DIP-251312X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8. Takeda K., Tonthat NK., Glover T., Xu W., Koonin EV., Yanagida M., Schumacher MA. Only protein-protein interactions imex curation takeda-2011-3 in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Takeda K., Tonthat NK., Glover T., Xu W., Koonin EV., Yanagida M., Schumacher MA. DIP original experiment: DIP-251313X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8. Takeda K., Tonthat NK., Glover T., Xu W., Koonin EV., Yanagida M., Schumacher MA. Only protein-protein interactions imex curation cut8_schpo Tethering factor for nuclear proteasome cut8 Cell untimely torn protein 8 cut8 SPAC17C9.13c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast METLSYSQIKKRKADFDEDISKRARQLPVGEQLPLSRLLQYSDKQQLFTILLQCVEKHPDLARDIRGILPAPSMDTCVETLRKLLINLNDSFPYGGDKRGDYAFNRIREKYMAVLHALNDMVPCYLPPYSTCFEKNITFLDAATNVVHELPEFHNPNHNVYKSQAYYELTGAWLVVLRQLEDRPVVPLLPLEELEEHNKTSQNRMEEALNYLKQLQKNEPLVHERSHTFQQTNPQNNFHRHTNSMNIGNDNGMGWHSMHQYI 20A7C1C554CD8C0C cut8-1 3230942 N/A 3230945 unspecified role unspecified role unspecified role unspecified role Region 1-217 Region 1-217 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cut8-2 3230943 N/A 3230945 unspecified role unspecified role unspecified role unspecified role Region 1-217 Region 1-217 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P38937:p.[Leu39Glu;Ile65Glu] L39E/I65E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cut8-3 3230944 N/A 3230945 unspecified role unspecified role unspecified role unspecified role Region 1-217 Region 1-217 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false