DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ de-2011-4 in vitro In vitro pull down pull down predetermined predetermined participant predetermined de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248891X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation de-2011-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248886X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation de-2011-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248888X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation de-2011-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248890X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation de-2011-8 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248887X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation de-2011-9 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248893X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation de-2011-10 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248889X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation de-2011-11 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. DIP original experiment: DIP-248892X Structure (London, England : 1993) 2011 IMEx Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. de Groot JC., Schlüter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss SM., Reichelt J., Standfuss-Gabisch C., Lesser CF., Leong JM., Heinz DW., Büssow K., Stradal TE. Only protein-protein interactions imex curation tir_eco27 Translocated intimin receptor Tir tir Secreted effector protein Tir espE E2348C_3941 protein protein eco27 Escherichia coli O127:H6 (strain E2348/69 / EPEC) MPIGNLGNNVNGNHLIPPAPPLPSQTDGAARGGTGHLISSTGALGSRSLFSPLRNSMADSVDSRDIPGLPTNPSRLAAATSETCLLGGFEVLHDKGPLDILNTQIGPSAFRVEVQADGTHAAIGEKNGLEVSVTLSPQEWSSLQSIDTEGKNRFVFTGGRGGSGHPMVTVASDIAEARTKILAKLDPDNHGGRQPKDVDTRSVGVGSASGIDDGVVSETHTSTTNSSVRSDPKFWVSVGAIAAGLAGLAATGIAQALALTPEPDDPTTTDPDQAANAAESATKDQLTQEAFKNPENQKVNIDANGNAIPSGELKDDIVEQIAQQAKEAGEVARQQAVESNAQAQQRYEDQHARRQEELQLSSGIGYGLSSALIVAGGIGAGVTTALHRRNQPAEQTTTTTTHTVVQQQTGGNTPAQGGTDATRAEDASLNRRDSQGSVASTHWSDSSSEVVNPYAEVGGARNSLSAHQPEEHIYDEVAADPGYSVIQNFSGSGPVTGRLIGTPGQGIQSTYALLANSGGLRLGMGGLTSGGESAVSSVNAAPTPGPVRFV q9uqb8-4 Brain-specific angiogenesis inhibitor 1-associated protein 2 BAIAP2-alpha BAIAP2 Fas ligand-associated factor 3 Insulin receptor substrate p53/p58 Insulin receptor substrate protein of 53 kDa protein protein human Homo sapiens Human MSLSRSEEMHRLTENVYKTIMEQFNPSLRNFIAMGKNYEKALAGVTYAAKGYFDALVKMGELASESQGSKELGDVLFQMAEVHRQIQNQLEEMLKSFHNELLTQLEQKVELDSRYLSAALKKYQTEQRSKGDALDKCQAELKKLRKKSQGSKNPQKYSDKELQYIDAISNKQGELENYVSDGYKTALTEERRRFCFLVEKQCAVAKNSAAYHSKGKELLAQKLPLWQQACADPSKIPERAVQLMQQVASNGATLPSALSASKSNLVISDPIPGAKPLPVPPELAPFVGRMSAQESTPIMNGVTGPDGEDYSPWADRKAAQPKSLSPPQSQSKLSDSYSNTLPVRKSVTPKNSYATTENKTLPRSSSMAAGLERNGRMRVKAIFSHAAGDNSTLLSFKEGDLITLLVPEARDGWHYGESEKTKMRGWFPFSYTRVLDSDGSDRLHMSLQQGKSSSTGNLLDKDDLAIPPPDYGAASRAFPAQTASGFKQRPYSVAVPAFSQGLDDYGARSMSSGSGTLVSTV tir_eco57 Translocated intimin receptor Tir tir espE Secreted effector protein Tir Z5112 ECs4561 protein protein eco57 Escherichia coli O157:H7 MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGSRALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQIGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGGAGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQTSTSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATETATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAIENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQTTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLLGNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV tir-baiap2-2 3230234 N/A 3230242 unspecified role unspecified role bait bait Region 446-460 Region 446-460 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro N/A 3230243 unspecified role unspecified role prey prey Q9UQB8-4:p.Phe196Ala F196A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Lys108Ala K108A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false baiap2-ehectir-1 3230235 N/A 3230244 unspecified role unspecified role unspecified role unspecified role Region 452-463 Region 452-463 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3230243 unspecified role unspecified role unspecified role unspecified role I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-tir-1 3230236 N/A 3230242 unspecified role unspecified role unspecified role unspecified role Region 448-459 Region 448-459 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3230243 unspecified role unspecified role unspecified role unspecified role I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ehectir-baiap2-1 3230237 N/A 3230244 unspecified role unspecified role bait bait Region 450-464 Region 450-464 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro N/A 3230243 unspecified role unspecified role prey prey Q9UQB8-4:p.Phe196Ala F196A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Lys108Ala K108A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false baiap2-ehectir-2 3230238 N/A 3230244 unspecified role unspecified role unspecified role unspecified role Region 452-463 Region 452-463 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q7DB77:p.Tyr458Phe Y458F mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3230243 unspecified role unspecified role unspecified role unspecified role I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-tir-2 3230239 N/A 3230242 unspecified role unspecified role prey prey Region 337-550 Region 337-550 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3230243 unspecified role unspecified role bait bait Q9UQB8-4:p.Phe196Ala F196A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Lys108Ala K108A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UQB8-4:p.Leu28Glu L28E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Arg193Ser R193S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Lys108Ala;Arg193Ser] K108A/R193S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Lys108Ala;Phe196Ala] K108A/F196A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false baiap2-ehectir-3 3230240 N/A 3230244 unspecified role unspecified role unspecified role unspecified role Region 452-463 Region 452-463 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3230243 unspecified role unspecified role unspecified role unspecified role I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Arg193Ser R193S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Lys108Ala K108A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Leu28Glu L28E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Phe196Ala F196A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-ehectir-4 3230241 N/A 3230244 unspecified role unspecified role prey prey Region 334-558 Region 334-558 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3230243 unspecified role unspecified role bait bait Q9UQB8-4:p.Phe196Ala F196A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false I-BAR domain Region 1-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Lys108Ala K108A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UQB8-4:p.Leu28Glu L28E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Arg193Ser R193S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Lys108Ala;Arg193Ser] K108A/R193S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Lys108Ala;Phe196Ala] K108A/F196A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false