DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ newman-2011-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. DIP original experiment: DIP-218864X Structure (London, England : 1993) 2011 IMEx Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1. Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. Only protein-protein interactions imex curation newman-2011-2 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. DIP original experiment: DIP-218868X Structure (London, England : 1993) 2011 IMEx Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1. Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. Only protein-protein interactions imex curation newman-2011-3 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. DIP original experiment: DIP-218865X Structure (London, England : 1993) 2011 IMEx Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1. Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. Only protein-protein interactions imex curation newman-2011-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. DIP original experiment: DIP-218869X Structure (London, England : 1993) 2011 IMEx Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1. Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. Only protein-protein interactions imex curation newman-2011-5 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. DIP original experiment: DIP-218866X Structure (London, England : 1993) 2011 IMEx Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1. Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. Only protein-protein interactions imex curation newman-2011-6 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. DIP original experiment: DIP-218867X Structure (London, England : 1993) 2011 IMEx Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1. Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. Only protein-protein interactions imex curation newman-2011-7 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. DIP original experiment: DIP-218870X Structure (London, England : 1993) 2011 IMEx Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1. Newman JA., Hewitt L., Rodrigues C., Solovyova A., Harwood CR., Lewis RJ. Only protein-protein interactions imex curation rnj1_bacsu Ribonuclease J1 rnjA ykqC BSU14530 protein protein bacsu Bacillus subtilis MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIVHTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQVIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKISAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV 48091B448B8431C9 rnj2_bacsu Ribonuclease J2 rnjB ymfA BSU16780 protein protein bacsu Bacillus subtilis MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERADRVKAIFLTHGHDENIGGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLREIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIVCTGDFKFDQTPALNQTCDIGEIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQQVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAGSQGEPLAALTRMANKAHKQLNIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQKRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHSKIAEETGMKRSDIFLIEKGDVVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHLVSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYEKTKRKPMIIPIIMEV 8EA92EE42D51F282 rnase j1-1 3230203 N/A 3230210 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rnase j1-rnase j2-1 3230204 N/A 3230210 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3230211 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rnase j1-2 3230205 N/A 3230210 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rnase j2-rnase j1-1 3230206 N/A 3230210 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3230211 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false rnase j1-3 3230207 N/A 3230210 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rnase j1-rnase j2-2 3230208 N/A 3230210 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3230211 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false rnase j2-1 3230209 N/A 3230211 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false