DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yu-2011-6 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. DIP original experiment: DIP-218709X Nature structural & molecular biology 2011 IMEx Mechanism of actin filament nucleation by the bacterial effector VopL. Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. Only protein-protein interactions imex curation yu-2011-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. DIP original experiment: DIP-218712X Nature structural & molecular biology 2011 IMEx Mechanism of actin filament nucleation by the bacterial effector VopL. Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. Only protein-protein interactions imex curation yu-2011-8 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. DIP original experiment: DIP-218710X Nature structural & molecular biology 2011 IMEx Mechanism of actin filament nucleation by the bacterial effector VopL. Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. Only protein-protein interactions imex curation yu-2011-9 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. DIP original experiment: DIP-218713X Nature structural & molecular biology 2011 IMEx Mechanism of actin filament nucleation by the bacterial effector VopL. Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. Only protein-protein interactions imex curation yu-2011-10 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. DIP original experiment: DIP-218711X Nature structural & molecular biology 2011 IMEx Mechanism of actin filament nucleation by the bacterial effector VopL. Yu B., Cheng HC., Brautigam CA., Tomchick DR., Rosen MK. Only protein-protein interactions imex curation q87ge5_vibpa VPA1370 protein protein vibpa vibpa MLKIKLPQQTSLAPSSETTQRLPVKISIKSICNKSICKTLHSLADKCHRFSKEIKQRSANHTPSSSHENLTTINLKSQNSAATFESTVELSIKHGSSIPPAPPLPGAIPPAPPLNLSKGAPKSSSNSLDSVNEDHSKLMEQIRQGVKLKSATKSLSADKSSADAHSKLMEELLTGGRKLKKVATSDIPAPPPLPSASTSKSPDSRNALLSEIAGFSKDRLRKTGSLETLNSSQSKDKESFEPTTHERLLSEDLFKQSPKLSEQELDELANNLADYLFQAADIDWHQVISEKTRGLTTEEMAKSEHRYVQAFCREILKYPDCYKSADVASPESPKSGGGSVIDVALKRLQTGRERLFTTTDEKGNRELKKGDAILESAINAARMAISTEEKNTILSNNVKSATFEVFCELPCMDGFAEQNGKTAFYALRAGFYSAFKNTDTAKQDITKFMKDNLQAGFSGYSYQGLTNRVAQLEAQLAALSAKLS D1A6437F04567F2F acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF vopl-1 3229825 N/A 3229830 unspecified role unspecified role unspecified role unspecified role VCD domain Region 247-484 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-vopl-3 3229826 N/A 3229831 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3229830 unspecified role unspecified role unspecified role unspecified role Region 129-157 Region 129-157 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Region 158-187 Region 158-187 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Region 199-226 Region 199-226 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false vopl-2 3229827 N/A 3229830 unspecified role unspecified role unspecified role unspecified role VCD domain Region 247-484 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region Δ461-484 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-vopl-4 3229828 N/A 3229831 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3229830 unspecified role unspecified role unspecified role unspecified role Region 115-484 Region 115-484 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q87GE5:p.[Lys323Glu;Arg347Glu;Arg354Glu] K323E/R347E/R354E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q87GE5:p.[Asp326Gly;Val327Gly;Pro333Gly] D326G/V327G/P333G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q87GE5:p.[Lys421Ala;Tyr425Ala;Arg428Asp] K421A/Y425A/R428D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false Deletion of region 461-484 decreased the interaction rate/strength. vopl-3 3229829 N/A 3229830 unspecified role unspecified role unspecified role unspecified role VCD domain Region 247-484 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region Δ461-484 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false