DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ungureanu-2011-1 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. DIP original experiment: DIP-218676X Nature structural & molecular biology 2011 IMEx The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling. Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. Only protein-protein interactions imex curation ungureanu-2011-2 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. DIP original experiment: DIP-218679X Nature structural & molecular biology 2011 IMEx The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling. Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. Only protein-protein interactions imex curation ungureanu-2011-3 in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. DIP original experiment: DIP-218680X Nature structural & molecular biology 2011 IMEx The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling. Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. Only protein-protein interactions imex curation ungureanu-2011-4 in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. DIP original experiment: DIP-218677X Nature structural & molecular biology 2011 IMEx The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling. Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. Only protein-protein interactions imex curation ungureanu-2011-5 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. DIP original experiment: DIP-218678X Nature structural & molecular biology 2011 IMEx The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling. Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. Only protein-protein interactions imex curation ungureanu-2011-6 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. DIP original experiment: DIP-218681X Nature structural & molecular biology 2011 IMEx The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling. Ungureanu D., Wu J., Pekkala T., Niranjan Y., Young C., Jensen ON., Xu CF., Neubert TA., Skoda RC., Hubbard SR., Silvennoinen O. Only protein-protein interactions imex curation jak2_human Tyrosine-protein kinase JAK2 Janus kinase 2 JAK2 protein protein human Homo sapiens Human MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKSEADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNHVFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVMSYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFLPKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPGSGPSGEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGKNLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNCHGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCPPKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG jak2-1 3229352 N/A 3229358 putative self putative self unspecified role unspecified role pseudokinase domain Region 545-809 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O60674:p.Lys581Ala K581A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false jak2-3 3229353 N/A 3229358 putative self putative self unspecified role unspecified role kinase domain Region 849-1124 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false jak2-4 3229354 N/A 3229358 putative self putative self unspecified role unspecified role pseudokinase domain Region 545-809 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false pSer523 pSer523 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false O60674:p.Lys581Ala K581A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false jak2-5 3229355 N/A 3229358 putative self putative self unspecified role unspecified role pseudokinase domain Region 545-809 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false jak2-6 3229356 N/A 3229358 putative self putative self unspecified role unspecified role pseudokinase domain Region 545-809 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O60674:p.Lys581Ala K581A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro phosphorylation phosphorylation reaction phosphorylation false false false jak2-7 3229357 N/A 3229358 putative self putative self unspecified role unspecified role pseudokinase domain Region 545-809 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false pSer523 pSer523 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false pTyr570 pTyr570 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false