DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ calabrese-2011-1 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. DIP original experiment: DIP-218650X Nature structural & molecular biology 2011 IMEx A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. Only protein-protein interactions imex curation calabrese-2011-2 in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. DIP original experiment: DIP-218654X Nature structural & molecular biology 2011 IMEx A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. Only protein-protein interactions imex curation calabrese-2011-3 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. DIP original experiment: DIP-218656X Nature structural & molecular biology 2011 IMEx A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. Only protein-protein interactions imex curation calabrese-2011-4 in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. DIP original experiment: DIP-218653X Nature structural & molecular biology 2011 IMEx A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. Only protein-protein interactions imex curation calabrese-2011-5 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. DIP original experiment: DIP-218651X Nature structural & molecular biology 2011 IMEx A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. Only protein-protein interactions imex curation calabrese-2011-6 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. DIP original experiment: DIP-218655X Nature structural & molecular biology 2011 IMEx A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. Only protein-protein interactions imex curation calabrese-2011-7 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. DIP original experiment: DIP-218652X Nature structural & molecular biology 2011 IMEx A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Calabrese MF., Scott DC., Duda DM., Grace CR., Kurinov I., Kriwacki RW., Schulman BA. Only protein-protein interactions imex curation rbx1_human E3 ubiquitin-protein ligase RBX1 RBX1 RNF75 ROC1 Regulator of cullins 1 RING finger protein 75 Protein ZYP RING-box protein 1 E3 ubiquitin-protein transferase RBX1 protein protein human Homo sapiens Human MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH cul1_human Cullin-1 CUL1 protein protein human Homo sapiens Human MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA ubc12_yeast NEDD8-conjugating enzyme UBC12 UBC12 YLR306W L2142.3 RUB1-conjugating enzyme Ubiquitin carrier protein 12 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLKLRQLQKKKQKENENSSSIQPNLSAARIRLKRDLDSLDLPPTVTLNVITSPDSADRSQSPKLEVIVRPDEGYYNYGSINFNLDFNEVYPIEPPKVVCLKKIFHPNIDLKGNVCLNILREDWSPALDLQSIITGLLFLFLEPNPNDPLNKDAAKLLCEGEKEFAEAVRLTMSGGSIEHVKYDNIVSP BEEECF40B82C4BAB ubc12_human NEDD8-conjugating enzyme Ubc12 UBE2M UBC12 Ubiquitin-conjugating enzyme E2 M NEDD8 carrier protein protein protein human Homo sapiens Human MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK nedd8_human NEDD8 Ubiquitin-like protein Nedd8 Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 NEDD8 protein protein human Homo sapiens Human MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ cul1-rbx1-17 3228520 N/A 3228527 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3228528 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 411-776 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cul1-ubc12-1 3228521 N/A 3228529 unspecified role unspecified role unspecified role unspecified role P52491:p.Asn146Cys N146C mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228528 unspecified role unspecified role unspecified role unspecified role Q13616:p.Arg717Cys R717C mutation mutation certain certain sequence position certain certain certain sequence position certain false C-terminal domain Region 411-776 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nedd8-ube2m-1 3228522 N/A 3228530 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3228531 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cul1-ubc12-2 3228523 N/A 3228529 unspecified role unspecified role unspecified role unspecified role P52491:p.Glu121Cys E121C mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228528 unspecified role unspecified role unspecified role unspecified role Q13616:p.Arg717Cys R717C mutation mutation certain certain sequence position certain certain certain sequence position certain false C-terminal domain Region 411-776 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cul1-rbx1-18 3228524 N/A 3228527 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3228528 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 411-776 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cul1-nedd8-5 3228525 N/A 3228531 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3228528 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 411-776 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rbx1-ube2m-3 3228526 N/A 3228530 unspecified role unspecified role unspecified role unspecified role Core domain Region 27-183 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228527 unspecified role unspecified role unspecified role unspecified role RING domain Region 36-108 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false