DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ iwase-2011-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. DIP original experiment: DIP-218606X Nature structural & molecular biology 2011 IMEx ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. Only protein-protein interactions imex curation iwase-2011-2 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. DIP original experiment: DIP-218605X Nature structural & molecular biology 2011 IMEx ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. Only protein-protein interactions imex curation iwase-2011-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. DIP original experiment: DIP-218608X Nature structural & molecular biology 2011 IMEx ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. Only protein-protein interactions imex curation iwase-2011-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. DIP original experiment: DIP-218607X Nature structural & molecular biology 2011 IMEx ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. Only protein-protein interactions imex curation iwase-2011-5 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. DIP original experiment: DIP-218610X Nature structural & molecular biology 2011 IMEx ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. Only protein-protein interactions imex curation iwase-2011-6 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. DIP original experiment: DIP-218609X Nature structural & molecular biology 2011 IMEx ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. Only protein-protein interactions imex curation iwase-2011-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. DIP original experiment: DIP-218611X Nature structural & molecular biology 2011 IMEx ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Iwase S., Xiang B., Ghosh S., Ren T., Lewis PW., Cochrane JC., Allis CD., Picketts DJ., Patel DJ., Li H., Shi Y. Only protein-protein interactions imex curation h3k9-2 Histone H3 N-terminal Peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLA p46100-1 Transcriptional regulator ATRX ATRX RAD54L XH2 X-linked helicase II ATP-dependent helicase ATRX X-linked nuclear protein Znf-HX protein protein human Homo sapiens Human MTAEPMSESKLNTLVQKLHDFLAHSSEESEETSSPPRLAMNQNTDKISGSGSNSDMMENSKEEGTSSSEKSKSSGSSRSKRKPSIVTKYVESDDEKPLDDETVNEDASNENSENDITMQSLPKGTVIVQPEPVLNEDKDDFKGPEFRSRSKMKTENLKKRGEDGLHGIVSCTACGQQVNHFQKDSIYRHPSLQVLICKNCFKYYMSDDISRDSDGMDEQCRWCAEGGNLICCDFCHNAFCKKCILRNLGRKELSTIMDENNQWYCYICHPEPLLDLVTACNSVFENLEQLLQQNKKKIKVDSEKSNKVYEHTSRFSPKKTSSNCNGEEKKLDDSCSGSVTYSYSALIVPKEMIKKAKKLIETTANMNSSYVKFLKQATDNSEISSATKLRQLKAFKSVLADIKKAHLALEEDLNSEFRAMDAVNKEKNTKEHKVIDAKFETKARKGEKPCALEKKDISKSEAKLSRKQVDSEHMHQNVPTEEQRTNKSTGGEHKKSDRKEEPQYEPANTSEDLDMDIVSVPSSVPEDIFENLETAMEVQSSVDHQGDGSSGTEQEVESSSVKLNISSKDNRGGIKSKTTAKVTKELYVKLTPVSLSNSPIKGADCQEVPQDKDGYKSCGLNPKLEKCGLGQENSDNEHLVENEVSLLLEESDLRRSPRVKTTPLRRPTETNPVTSNSDEECNETVKEKQKLSVPVRKKDKRNSSDSAIDNPKPNKLPKSKQSETVDQNSDSDEMLAILKEVSRMSHSSSSDTDINEIHTNHKTLYDLKTQAGKDDKGKRKRKSSTSGSDFDTKKGKSAKSSIISKKKRQTQSESSNYDSELEKEIKSMSKIGAARTTKKRIPNTKDFDSSEDEKHSKKGMDNQGHKNLKTSQEGSSDDAERKQERETFSSAEGTVDKDTTIMELRDRLPKKQQASASTDGVDKLSGKEQSFTSLEVRKVAETKEKSKHLKTKTCKKVQDGLSDIAEKFLKKDQSDETSEDDKKQSKKGTEEKKKPSDFKKKVIKMEQQYESSSDGTEKLPEREEICHFPKGIKQIKNGTTDGEKKSKKIRDKTSKKKDELSDYAEKSTGKGDSCDSSEDKKSKNGAYGREKKRCKLLGKSSRKRQDCSSSDTEKYSMKEDGCNSSDKRLKRIELRERRNLSSKRNTKEIQSGSSSSDAEESSEDNKKKKQRTSSKKKAVIVKEKKRNSLRTSTKRKQADITSSSSSDIEDDDQNSIGEGSSDEQKIKPVTENLVLSSHTGFCQSSGDEALSKSVPVTVDDDDDDNDPENRIAKKMLLEEIKANLSSDEDGSSDDEPEEGKKRTGKQNEENPGDEEAKNQVNSESDSDSEESKKPRYRHRLLRHKLTVSDGESGEEKKTKPKEHKEVKGRNRRKVSSEDSEDSDFQESGVSEEVSESEDEQRPRTRSAKKAELEENQRSYKQKKKRRRIKVQEDSSSENKSNSEEEEEEKEEEEEEEEEEEEEEEDENDDSKSPGKGRKKIRKILKDDKLRTETQNALKEEEERRKRIAEREREREKLREVIEIEDASPTKCPITTKLVLDEDEETKEPLVQVHRNMVIKLKPHQVDGVQFMWDCCCESVKKTKKSPGSGCILAHCMGLGKTLQVVSFLHTVLLCDKLDFSTALVVCPLNTALNWMNEFEKWQEGLKDDEKLEVSELATVKRPQERSYMLQRWQEDGGVMIIGYEMYRNLAQGRNVKSRKLKEIFNKALVDPGPDFVVCDEGHILKNEASAVSKAMNSIRSRRRIILTGTPLQNNLIEYHCMVNFIKENLLGSIKEFRNRFINPIQNGQCADSTMVDVRVMKKRAHILYEMLAGCVQRKDYTALTKFLPPKHEYVLAVRMTSIQCKLYQYYLDHLTGVGNNSEGGRGKAGAKLFQDFQMLSRIWTHPWCLQLDYISKENKGYFDEDSMDEFIASDSDETSMSLSSDDYTKKKKKGKKGKKDSSSSGSGSDNDVEVIKVWNSRSRGGGEGNVDETGNNPSVSLKLEESKATSSSNPSSPAPDWYKDFVTDADAEVLEHSGKMVLLFEILRMAEEIGDKVLVFSQSLISLDLIEDFLELASREKTEDKDKPLIYKGEGKWLRNIDYYRLDGSTTAQSRKKWAEEFNDETNVRGRLFIISTKAGSLGINLVAANRVIIFDASWNPSYDIQSIFRVYRFGQTKPVYVYRFLAQGTMEDKIYDRQVTKQSLSFRVVDQQQVERHFTMNELTELYTFEPDLLDDPNSEKKKKRDTPMLPKDTILAELLQIHKEHIVGYHEHDSLLDHKEEEELTEEERKAAWAEYEAEKKGLTMRFNIPTGTNLPPVSFNSQTPYIPFNLGALSAMSNQQLEDLINQGREKVVEATNSVTAVRIQPLEDIISAVWKENMNLSEAQVQALALSRQASQELDVKRREAIYNDVLTKQQMLISCVQRILMNRRLQQQYNQQQQQQMTYQQATLGHLMMPKPPNLIMNPSNYQQIDMRGMYQPVAGGMQPPPLQRAPPPMRSKNPGPSQGKSM h3-atrx-1 3227294 N/A 3227301 unspecified role unspecified role bait bait Region 1-14 Region 1-14 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3227302 unspecified role unspecified role prey prey ADD domain Region 162-293 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3-atrx-2 3227295 N/A 3227301 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false in vitro In vitro N/A 3227302 unspecified role unspecified role prey prey ADD domain Region 162-293 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Gln219Pro Q219P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.His189Asn H189N mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Glu252Leu E252L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Arg246Cys R246C mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Pro190Ala P190A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr203Ala Y203A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr204Ala Y204A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Asp207Ala D207A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false atrx-h3-3 3227296 N/A 3227301 unspecified role unspecified role unspecified role unspecified role Region 1-10 Region 1-10 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3227302 unspecified role unspecified role unspecified role unspecified role ADD domain Region 167-289 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false atrx-h3-4 3227297 N/A 3227301 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3227302 unspecified role unspecified role unspecified role unspecified role ADD domain Region 167-289 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3-atrx-3 3227298 N/A 3227301 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false K9me2 K9me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3227302 unspecified role unspecified role prey prey ADD domain Region 162-293 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Gln219Pro Q219P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.His189Asn H189N mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Glu252Leu E252L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Arg246Cys R246C mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Pro190Ala P190A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr203Ala Y203A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr204Ala Y204A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Asp207Ala D207A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3-atrx-4 3227299 N/A 3227301 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false K9me1 K9me1 n6me1lys N6-methyl-L-lysine (2S)-2-amino-6-methylaminohexanoic acid MOD_RES N6-methyllysine N(zeta)-methyllysine N6-methyl-L-lysine N6-methylated L-lysine epsilon-methyllysine Methyl Methylation N-methyl Lysyl N6Me1Lys certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3227302 unspecified role unspecified role prey prey ADD domain Region 162-293 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Gln219Pro Q219P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.His189Asn H189N mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Glu252Leu E252L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Arg246Cys R246C mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Pro190Ala P190A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr203Ala Y203A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr204Ala Y204A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Asp207Ala D207A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3-atrx-5 3227300 N/A 3227301 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false K9me3 K9me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3227302 unspecified role unspecified role prey prey ADD domain Region 162-293 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Gln219Pro Q219P mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.His189Asn H189N mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Glu252Leu E252L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Arg246Cys R246C mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Pro190Ala P190A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr203Ala Y203A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Tyr204Ala Y204A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46100-1:p.Asp207Ala D207A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false