DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ barberon-2011-1 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barberon M., Zelazny E., Robert S., Conéjéro G., Curie C., Friml J., Vert G. DIP original experiment: DIP-253895X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Monoubiquitin-dependent endocytosis of the iron-regulated transporter 1 (IRT1) transporter controls iron uptake in plants. Barberon M., Zelazny E., Robert S., Conéjéro G., Curie C., Friml J., Vert G. Only protein-protein interactions imex curation barberon-2011-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Barberon M., Zelazny E., Robert S., Conéjéro G., Curie C., Friml J., Vert G. DIP original experiment: DIP-253896X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Monoubiquitin-dependent endocytosis of the iron-regulated transporter 1 (IRT1) transporter controls iron uptake in plants. Barberon M., Zelazny E., Robert S., Conéjéro G., Curie C., Friml J., Vert G. Only protein-protein interactions imex curation barberon-2011-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Barberon M., Zelazny E., Robert S., Conéjéro G., Curie C., Friml J., Vert G. DIP original experiment: DIP-253897X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Monoubiquitin-dependent endocytosis of the iron-regulated transporter 1 (IRT1) transporter controls iron uptake in plants. Barberon M., Zelazny E., Robert S., Conéjéro G., Curie C., Friml J., Vert G. Only protein-protein interactions imex curation ubiq_arath Ubiquitin UBQ1 UBQ2 UBQ3 UBQ4 UBQ5 UBQ6 UBQ7 UBQ10 UBQ11 UBQ14 UBQ15 UBQ16 RPL40B RPL40A RPS27AC RPS27AB RUB2 RUB1 RPS27AA F3C22.8 F22O6_30 F9C22.10 F15A17_270 MOK16.15 T1M15.20 T17J13.210 F14M4.6 T20F21.24 C17L7.240 T32N4.13 T5J8.21 T19E23.13 F28C11.5 F26F24.28 At3g52590 At2g36170 At5g03240 At5g20620 At3g62250 At2g47110 At2g35635 At4g05320 At4g05050 At4g02890 At1g31340 At1g23410 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG irt1_arath Fe(2+) transport protein 1 IRT1 Fe(II) transport protein 1 Iron-regulated transporter 1 T16H5.50 At4g19690 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASNSALLMKTIFLVLIFVSFAISPATSTAPEECGSESANPCVNKAKALPLKVIAIFVILIASMIGVGAPLFSRNVSFLQPDGNIFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFSGFLAMLSGLITLAIDSMATSLYTSKNAVGIMPHGHGHGHGPANDVTLPIKEDDSSNAQLLRYRVIAMVLELGIIVHSVVIGLSLGATSDTCTIKGLIAALCFHQMFEGMGLGGCILQAEYTNMKKFVMAFFFAVTTPFGIALGIALSTVYQDNSPKALITVGLLNACSAGLLIYMALVDLLAAEFMGPKLQGSIKMQFKCLIAALLGCGGMSIIAKWA irt1-ub-1 3226791 N/A 3226794 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3226795 unspecified role unspecified role bait bait arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false irt1-ub-2 3226792 N/A 3226794 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3226795 unspecified role unspecified role bait bait Region 146-194 Region 146-194 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false irt1-ub-3 3226793 N/A 3226794 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3226795 unspecified role unspecified role bait bait Region 146-194 Region 146-194 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false