IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ luo-2011-8 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Luo X., Li Z., Li X., Wang G., Liu W., Dong S., Cai S., Tao D., Yan Q., Wang J., Leng Y., Gong J., Hu J. jbhu@tjh.tjmu.edu.cn,jpgong@tjh.tjmu.edu.cn human fetal liver International journal of molecular medicine 2011 imex curation Only protein-protein interactions imex curation luo-2011-9 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Luo X., Li Z., Li X., Wang G., Liu W., Dong S., Cai S., Tao D., Yan Q., Wang J., Leng Y., Gong J., Hu J. jbhu@tjh.tjmu.edu.cn,jpgong@tjh.tjmu.edu.cn human fetal liver International journal of molecular medicine 2011 imex curation Only protein-protein interactions imex curation luo-2011-10 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Luo X., Li Z., Li X., Wang G., Liu W., Dong S., Cai S., Tao D., Yan Q., Wang J., Leng Y., Gong J., Hu J. jbhu@tjh.tjmu.edu.cn,jpgong@tjh.tjmu.edu.cn International journal of molecular medicine 2011 imex curation Only protein-protein interactions imex curation luo-2011-11 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Luo X., Li Z., Li X., Wang G., Liu W., Dong S., Cai S., Tao D., Yan Q., Wang J., Leng Y., Gong J., Hu J. jbhu@tjh.tjmu.edu.cn,jpgong@tjh.tjmu.edu.cn International journal of molecular medicine 2011 imex curation Only protein-protein interactions imex curation luo-2011-12 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Luo X., Li Z., Li X., Wang G., Liu W., Dong S., Cai S., Tao D., Yan Q., Wang J., Leng Y., Gong J., Hu J. jbhu@tjh.tjmu.edu.cn,jpgong@tjh.tjmu.edu.cn International journal of molecular medicine 2011 imex curation Only protein-protein interactions imex curation sav1_human Protein salvador homolog 1 SAV1 WW45 45 kDa WW domain protein protein protein human Homo sapiens Human MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDICLPDSSPNAFSTSGDVVSRNQSFLRTPIQRTPHEIMRRESNRLSAPSYLARSLADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFFDGQRKRPLGDRAHEDYRYYEYNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF hax1_human HCLS1-associated protein X-1 HAX1 HS1BP1 HS1-associating protein X-1 HS1-binding protein 1 protein protein human Homo sapiens Human MSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEEFGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPRTREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTVTRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR sav1-hax1-2 3316629 N/A 3316634 unspecified role unspecified role bait bait N/A 3316635 unspecified role unspecified role prey prey physical association physical association false false false no figure sav1-hax1-3 3316630 N/A 3316634 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3316635 unspecified role unspecified role prey prey naturally occurring naturally occurring endogenous endogenous physical association physical association false false false Figure 1 sav1-hax1-1 3316631 N/A 3316634 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3316635 unspecified role unspecified role bait bait naturally occurring naturally occurring endogenous endogenous physical association physical association false false false Figure 1 sav1-4 3316632 N/A 3316635 unspecified role unspecified role neutral component neutral component naturally occurring naturally occurring endogenous endogenous N/A 3316634 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 1D hax1-sav1-2 3316633 N/A 3316634 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 3316635 unspecified role unspecified role bait bait purified purified c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 2